Spider silk nerve graft promotes axonal regeneration on long distance nerve defect in a sheep model

Kornfeld, Tim; Neßler, Jasmin; Helmer, Carina; Hannemann, R.; Waldmann, Karl-Heinz; Peck, Claas‐Tido; Hoffmann, Peter; Brandes, Gudrun; Vogt, Peter M.; Radtke, Christine

doi:10.1016/j.biomaterials.2021.120692

Cited by 36 publications

(43 citation statements)

References 60 publications

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“…In this part of the study, we also included native dragline silk from L. sclopetarius, since dragline silk facilitates cell adhesion and neurite growth both in vitro and in vivo. 23,24,38,61,62 For cells cultured on the three fiber types (NT2RepCT, VN-NT2RepCT, and native dragline silk), we found no significant differences in viability or mean length of neurites that grew along the fibers. However, another important parameter to consider is the potential of the fibers to guide neurites and to keep them from deviating from the direction of their intended target.…”

Section: Discussionmentioning

confidence: 68%

“…Spider silk is an interesting biomaterial for nerve regeneration that in several studies using large animal models of critically sized nerve defects have shown excellent ability to mediate regeneration of peripheral nerves. 23,24,38 However, for clinical applications, a chemically defined material is needed. Previously, we developed NT2RepCT that can be produced at high yields and can be spun into continuous fibers using extrusion of a 300 mg/ml NT2RepCT solution into a pH 5 aqueous buffer.…”

Section: Discussionmentioning

confidence: 99%

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Artificial spider silk supports and guides neurite extension in vitro

et al. 2021

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Artificial spider silk supports and guides neurite extension in vitro

et al. 2021

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“…Especially Saheb-al-Zamani et al, 2013 [49] and Brenner et al, 2005 [44] were able to demonstrate a positive effect on nerve regeneration by adding autologous or MHC-matched Schwann cells to cold stored nerve allografts. Recently, Kornfeld et al [71] were able to demonstrate a delay in nerve regeneration by a time consuming repopulation of acellular nerve grafts by autologous Schwann cells compared to isograft control.…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of Critical Nerve Defects Using Allogenic Nerve Tissue: A Review of Current Approaches

Kornfeld

Borger

Radtke

2021

IJMS

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Regardless of the nerve defect length, nerve injury is a debilitating condition for the affected patient that results in loss of sensory and motor function. These functional impairments can have a profound impact on the patient’s quality of life. Surgical approaches for the treatment of short segment nerve defects are well-established. Autologous nerve transplantation, considered the gold standard, and the use of artificial nerve grafts are safe and successful procedures for short segment nerve defect reconstruction. Long segment nerve defects which extend 3.0 cm or more are more problematic for repair. Methods for reconstruction of long defects are limited. Artificial nerve grafts often fail to regenerate and autologous nerve grafts are limited in length and number. Cadaveric processed/unprocessed nerve allografts are a promising alternative in nerve surgery. This review gives a systematic overview on pre-clinical and clinical approaches in nerve allograft transplantation.

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“…Surprisingly, the axonal regeneration rate occurred on a spidroin-based implant with a directional velocity of 1.309 mm/d, close to that of autologous nerve transplant (1.57 mm/d, the ideal axonal regeneration rate). The implanted spider silks could be fully degraded through the photoimmune response mainly mediated by Langerhans giant cells within 3 months, so that the constructs could be replaced by an autologous matrix [ 55 ].…”

Section: Spidroin-based Biomaterials In Different Dimensionsmentioning

confidence: 99%

Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies

Zhang

Wang

et al. 2021

Stem Cells International

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Spider silks are increasingly gaining interest for potential use as biomaterials in tissue engineering and biomedical applications. Owing to their facile and versatile processability in native and regenerated forms, they can be easily tuned via chemical synthesis or recombinant technologies to address specific issues required for applications. In the past few decades, native spider silk and recombinant silk materials have been explored for a wide range of applications due to their superior strength, toughness, and elasticity as well as biocompatibility, biodegradation, and nonimmunogenicity. Herein, we present an overview of the recent advances in spider silk protein that fabricate biomaterials for tissue engineering and regenerative medicine. Beginning with a brief description of biological and mechanical properties of spidroin-based materials and the cellular regulatory mechanism, this review summarizes various types of spidroin-based biomaterials from genetically engineered spider silks and their prospects for specific biomedical applications (e.g., lung tissue engineering, vascularization, bone and cartilage regeneration, and peripheral nerve repair), and finally, we prospected the development direction and manufacturing technology of building more refined and customized spidroin-based protein scaffolds.

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Spider silk nerve graft promotes axonal regeneration on long distance nerve defect in a sheep model

Cited by 36 publications

References 60 publications

Artificial spider silk supports and guides neurite extension in vitro

Artificial spider silk supports and guides neurite extension in vitro

Reconstruction of Critical Nerve Defects Using Allogenic Nerve Tissue: A Review of Current Approaches

Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies

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