2014
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Abstract: This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro. Ovarian fragments were cultured for 1 or 7 days in Minimum Essential Medium with different S1P concentrations (0, 1, 10, 50, 100 or 200ng/mL). All ovarian fragments were processed for histological analysis in optical microscopy, transmission electron microscopy and fluorescence analysis. The treatment using 1ng/mL of S1… Show more

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“…The ability to support oocyte development entirely in vitro would offer an alternative strategy avoiding both the risk of cancer cells reintroduction and the need for the second surgery for transplantation. Moreover, primordial follicles culture would nullify the need for hormonal stimulation after ACOTT [78,79]. The ovarian tissue that is recovered for cryopreservation mainly contains follicles in the early stage of development [80].…”
Section: Future Possibilitiesmentioning
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“…The ability to support oocyte development entirely in vitro would offer an alternative strategy avoiding both the risk of cancer cells reintroduction and the need for the second surgery for transplantation. Moreover, primordial follicles culture would nullify the need for hormonal stimulation after ACOTT [78,79]. The ovarian tissue that is recovered for cryopreservation mainly contains follicles in the early stage of development [80].…”
Section: Future Possibilitiesmentioning