2011
DOI: 10.1073/pnas.1115484108
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Abstract: The mechanism of action of 2-hydroxyoleic acid (2OHOA), a potent antitumor compound, has not yet been fully elucidated. Here, we show that human cancer cells have markedly lower levels of sphingomyelin (SM) than nontumor (MRC-5) cells. In this context, 2OHOA treatment strongly augments SM mass (4.6-fold), restoring the levels found in MRC-5 cells, while a loss of phosphatidylethanolamine and phosphatidylcholine is observed (57 and 30%, respectively). The increased SM mass was due to a rapid and highly specific… Show more

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Cited by 141 publications
(166 citation statements)
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“…In this context, very low levels of SM were found in human glioma (SF767) cells when compared with normal (MRC-5) cells, a characteristic common to all the cancer cell lines that we have studied to date (leukemia, lung cancer, and other glioma cells; ref. 26). In SF767 cells, 2OHOA treatments induced restoration of SM to levels similar to those observed in nontumor cells (Fig.…”
Section: Discussionmentioning
confidence: 85%
See 1 more Smart Citation
“…In this context, very low levels of SM were found in human glioma (SF767) cells when compared with normal (MRC-5) cells, a characteristic common to all the cancer cell lines that we have studied to date (leukemia, lung cancer, and other glioma cells; ref. 26). In SF767 cells, 2OHOA treatments induced restoration of SM to levels similar to those observed in nontumor cells (Fig.…”
Section: Discussionmentioning
confidence: 85%
“…In any case, additional molecular events/mechanisms should not be discarded. Indeed, we have recently seen that 2OHOA also induces marked increases in the levels of nuclear SM (26). In this context, nuclear phospholipids have been shown to participate in nuclear signaling and could account for some of the cellular effects induced by 2OHOA including glioma cell proliferation, differentiation and death (38).…”
Section: Discussionmentioning
confidence: 99%
“…8). Lipid intake has been shown to modify the composition of cell membranes, regulating their biophysical properties and influencing protein-membrane interactions, thereby affecting cell signaling, metabolism, and viability (Spector and Burns, 1987;Barceló-Coblijn et al, 2011;Terés et al, 2012). HTO and triolein induce changes in membrane lipid composition and structure that might be associated with their antitumor activity, as shown previously for other anticancer drugs (Martínez et al, 2005).…”
Section: Discussionmentioning
confidence: 96%
“…The detection of PM level of SM was performed by lysenin (PeptaNova) staining as described by Yamaji et al ( 33 ) and modifi ed by Barceló-Coblijn et al ( 34 ). Lysenin was detected by using a 1:200 rabbit anti-lysenin antibody (PeptaNova) and a 1:200 F(ab´) 2 fragment of goat anti-rabbit IgG, Alexa Fluor 647 conjugated (Molecular Probes).…”
Section: Fluorescence Microscopymentioning
confidence: 99%