Sphingobacterium chungjuense sp. nov., isolated from a freshwater lake

Abstract

A Gram-stain-negative, rod-shaped, aerobic, non-flagellated, chemoheterotrophic bacterium, designated strain IMCC25678T, was isolated from an artificial freshwater reservoir, Chungju Lake, in the Republic of Korea. The 16S rRNA gene sequence analysis indicated that strain IMCC25678T belongs to the genus Sphingobacterium with ≤98.7 % sequence similarities to Sphingobacterium species. Whole genome sequencing of strain IMCC25678T revealed a 3.9 Mbp genome size with a DNA G+C content of 42.2 mol%. The IMCC25678T genome shared ≤89.7 % average nucleotide identity and ≤21.4 % digital DNA–DNA hybridization values with closely related species of the genus Sphingobacterium , indicating that the strain represents a novel species. Summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), iso-C15 : 0 and iso-C17 : 0 3-OH were found to be the predominant cellular fatty acid constituents in the strain. The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, one unidentified phosphoglycolipid, one unidentified sphingolipid and three unidentified polar lipids. Based on the phylogenetic and phenotypic characteristics, strain IMCC25678T was considered to represent a novel species within the genus Sphingobacterium , for which the name Sphingobacterium chungjuense sp. nov. is proposed. The type strain is IMCC25678T (=KACC 19485T=NBRC 113130T).

Sphingobacterium faecale sp. nov., a 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from camel faeces

“…The predominant menaquinone of strain C459-1 T was MK-7, consistent with all known members of the genus Sphingobacterium [3,4,11,13,15,17,18]. The major fatty acids (>10.0 %) of strain C459-1 T comprised iso-C 15 : 0 (37.30 %), summed feature 3 (C 16 : 1 ω6c and/or C 16 : 1 ω7c; 32.9%) and iso-C 17 : 0 3-OH (10.4 %).…”

“…The fatty acid profiles of strain C459-1 T and the related reference species are shown in . Thus, the fatty acid profile, polar lipids and major isoprenoid quinone of strain C459-1 T were all typical of those of species within the genus Sphingobacterium [1, 3–6, 11, 13, 15, 17, 18].…”

“…At the time of writing, the genus Sphingobacterium comprises 63 validly described species (https://lpsn.dsmz.de/). Sphingobacterium species are widely distributed in soils [3][4][5][6][7], plant root rhizosphere [8][9][10][11], milk [12,13], faeces [14], lakes [15], compost [16], and other sources [17][18][19]. Members of this genus are characterized by Gram-stain-negative, rod-shaped, yellow-pigmented bacteria, strictly aerobic with DNA G+C contents in the range of 36.6-44.5 mol%, menaquinone-7 (MK-7) as the predominant respiratory quinone, iso-C 15 : 0 , C 16 : 1 ω7c and iso-C 17:0 3-OH as the major fatty acids, and phosphatidylethanolamine as the major component of the polar lipids [4].…”

Assessment of Chlorella sorokiniana Growth in Anaerobic Digester Effluent

“…Sphingomonads are used for a wide range of biotechnological applications, from the bioremediation of environmental pollutants to the production of extracellular polymers for food and other industries [ 41 ]. Species of the aerobic genus Sphingobacterium have mainly been isolated from soil, compost, sludge, plants, raw milk, and water [ 42 ]. They are also involved in biodegradation processes [ 43 ].…”

Sphingobacterium lumbrici sp. nov., a novel bacterium isolated from wormcast of Eisenia foetida

“…These species have been isolated from various environments including raw milk, plants, diverse soil habitats, faeces, activated sludge, clinical specimens, freshwater habitats and nodule surface of soybean and include Sphingobacterium terrae [4], Sphingobacterium bovisgrunnientis [5], Sphingobacterium tabacisoli [6], Sphingobacterium solani [7], Sphingobacterium composti [8], Sphingobacterium cellulitidis [9], Sphingobacterium yanglingense [10], Sphingobacterium suaedae [11], Sphingobacterium caeni [12] and Sphingobacterium corticibacter [13]. Recently, Sphingobacterium olei [14], Sphingobacterium chungjuense [15] and Sphingobacterium cavernae [16] were reported. During an investigation into diversity of plant growth-promoting bacteria in PR China, a bacterium, designated strain 1.3611 T , was isolated from wormcast of Eisenia foetida collected at an earthworm farm in Kunming, Yunnan Province, PR China.…”