Spermatozoa from normozoospermic fertile and infertile individuals convey a distinct mi<scp>RNA</scp> cargo

Salas‐Huetos, Albert; Blanco, Joan; Vidal, Francesca; Grossmann, Mark; Pons, Maria Carme; Garrido, Nicolás; Antón, Ester

doi:10.1111/andr.12276

Cited by 52 publications

(35 citation statements)

References 40 publications

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“…However, it is now understood that sperm sRNAs actively contribute to sperm function, early embryo development and transgenerational epigenetic inheritance. Therefore, sRNAs may potentially hold diagnostic value for assessment of the fertility status of a male (Salas-Huetos et al 2016). This potential has served as a catalyst for several recent studies that utilised either a microarray or high-throughput sequencing approach to catalogue the RNA profile of spermatozoa.…”

Section: Spermatozoa Harbour a Diverse Small Rna Profile That Is Potementioning

confidence: 99%

The contribution of epididymosomes to the sperm small RNA profile

2019

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It is now well established that mature spermatozoa harbour a rich and diverse profile of small non-protein-coding regulatory RNAs (sRNAs). There is also growing appreciation that this sRNA profile displays considerable plasticity, being altered in response to paternal exposure to a variety of environmental stressors. Coupled with evidence that upon delivery to the oocyte at the moment of fertilisation, sperm-borne sRNAs are able to influence both early embryonic development and the subsequent health of the offspring, there is now interest in both the timing and degree of change in the composition of the sRNA cargo of sperm. Models in which such epigenetic changes are linked to the spermatogenic cycle are seemingly incompatible with the lack of overt phenotypic changes in the spermatozoa of affected males. Rather, there is mounting consensus that such changes are imposed on sperm during their transit and storage within the epididymis, a protracted developmental window that takes place over several weeks. Notably, since spermatozoa are rendered transcriptionally and translationally silent during their development in the testes, it is most likely that the epididymis-documented alterations to the sperm sRNA profile are driven extrinsically, with a leading candidate being epididymosomes: small membrane enclosed extracellular vesicles that encapsulate a complex macromolecular cargo of proteins and RNAs, including the sRNAs. Here, we review the role of epididymosome–sperm communication in contributing to the establishment of the sperm sRNA profile during their epididymal transit.

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Section: Spermatozoa Harbour a Diverse Small Rna Profile That Is Potementioning

confidence: 99%

The contribution of epididymosomes to the sperm small RNA profile

2019

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“…Furthermore, the role of the particular miRNAs targeted genes has been found in the apoptosis, the integrity of the mitochondrial membrane and alteration in spermatogenesis, representing its role in fertility. Performing TaqMan Array Human MicroRNA of spermatozoa from the patients affirms that infertile and fertile persons convey different miRNA cargos pointing its role in male reproductive performance (Salas‐Huetos et al, ). Collectively, miRNAs play important role in regulating every step of male germ cell development, which comprises mitosis, meiosis and spermiogenesis (Mciver, Roman, Nixon, & Mclaughlin, ).…”

Section: Mirna and Spermatogenesismentioning

confidence: 94%

Epigenetics and oxidative stress: A twin‐edged sword in spermatogenesis

et al. 2019

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Spermatogenesis is a series of complex events involving a delicate balance between cell proliferation and cell differentiation. Aggregation of chromatins and epigenetic modifications play a vital role in spermatogenesis via regulation of molecular pathways to maintain testicular homeostasis. These epigenetic mechanisms consist of histone modification, chromatin remodelling, DNA methylation and miRNA, etc., which reportedly are critical players in spermatogenesis. One such mechanism involves regulation of oxidative stress in the male reproductive system. The fact that testicular cells contain plenty of unsaturated fatty acids and undergo division at a high rate makes spermatogenic cells highly susceptible to oxidative insult leading to deleterious effect on spermatozoa, which may culminate in infertility in men. Although the correlation between ROS‐mediated oxidative stress and epigenetic alterations has been indicated, research in this regard is still in infancy. Further, the fact that environmental and life style factors are critical determinants of spermatogenic potential indicates the importance of epigenetic regulation of key molecular events in spermatogenesis. Therefore, the current review aims to discuss the ROS‐induced epigenetic deregulation of the molecular mechanism(s) involved in spermatogenesis.

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“…Alteration in the sperm transcript level of miRNAs was reported in patients with different spermatogenic impairments 9‐13 . In addition, normozoospermic infertile patients exhibit a different miRNAs expression profile from normozoospermic fertile individuals 14 …”

Section: Introductionmentioning

confidence: 99%

Sperm miR‐26a‐5p and its target PTEN transcripts content in men with unexplained infertility

et al. 2020

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Background Recent evidence suggests that routine semen analysis has limited power to predict male fertility. Several efforts have been made to best discriminate between fertile and subfertile men using several sperm features. So, there is a need to more reliable assays for providing molecular data to evaluate sperm quality and predict male fertility. Objectives Present study aimed to investigate relationship between sperm miR‐26a‐5p and its target PTEN transcript content and sperm parameters in unexplained infertile and healthy fertile men. Materials and methods Sperm parameters and transcript content of miR‐26a‐5p and PTEN in ejaculated spermatozoa were assessed in fertile (n = 15) and normozoospermic infertile (n = 15) men. Results Sperm transcript levels of miR‐26a‐5p were lower in unexplained infertile men than in fertile controls (P = .021). High sperm PTEN expression is correlated with low miR‐26a‐5p transcript levels in ejaculated spermatozoa. High levels of sperm miR‐26a‐5p transcript were associated with high sperm motility and normal morphology. Receiver operating characteristic curve (ROC) analysis indicated miR‐26a‐5p has a fairly good diagnostic value to distinguish fertile and unexplained infertile men with an area under curve (AUC) of 0.747 (95% confidence interval: 0.560‐0.933; P = .021). Discussion This study suggests that sperm miR‐26a‐5p and its target PTEN transcript content may contribute to infertility etiology in unexplained infertile patients. Conclusion miR‐26a‐5p has a potential to use as a diagnostic biomarker and provide new therapeutic approaches for male infertility.

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Spermatozoa from normozoospermic fertile and infertile individuals convey a distinct miRNA cargo

Cited by 52 publications

References 40 publications

The contribution of epididymosomes to the sperm small RNA profile

The contribution of epididymosomes to the sperm small RNA profile

Epigenetics and oxidative stress: A twin‐edged sword in spermatogenesis

Sperm miR‐26a‐5p and its target PTEN transcripts content in men with unexplained infertility

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