Sperm velocity, strict morphology and the hypo-osmotic swelling test as predictors of the sperm fertilization potential: experience from the IVF model.

Sallam, Hassan; Agameya, Abdel-Fattah; Sallam, A. A.; Rahman, Ashraf Farrag; Ezzeldin, F.

doi:10.1016/s0015-0282(01)02776-5

Cited by 13 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…; this range of indentation rate is comparable with the typical speed of spermatozoa observed during 'in vitro' fertilization [21]. Following contact, the AFM tip exerts a force against the ZP proportionate to cantilever deflection.…”

supporting

confidence: 68%

“…Remarkably, all material properties determined with the identification procedure changed by less than 10% (table 1) in spite of the fact that the indentation rate varied by 20 times (i.e. from 0.5 to 10 mm s 21 ). This sounds logical because the visco-hyperelastic parameters describing the time-independent and time-dependent parts of the ZP's mechanical response are inherent properties of the investigated material.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

A hybrid characterization framework to determine the visco-hyperelastic properties of a porcine zona pellucida

et al. 2014

View full text Add to dashboard Cite

The zona pellucida (ZP) is a specialized extracellular matrix surrounding the developing oocyte. This thick matrix consists of various types of glycoprotein that play different roles in the fertilization process. Nowadays, several techniques are available for assessing ZP's mechanical response. The basic assumption behind these methods is that the ZP behaves like an elastic body: hence, dissipative forces are neglected and Young's modulus remains unaffected by probe dynamics. However, dissipative forces are strongly regulated by the slippage of ZP chains past one another while reaction forces related to elastic deformations (driven by the ability of each chain to stretch) depend on the ZP structure (i.e. number of cross-links and distances between knots). Although viscous reaction forces generated by the ZP are one of the main factors regulating sperm transit, their peculiar behaviour along the ZP structure remains poorly understood and rarely investigated. In order to overcome this limitation, a novel visco-hyperelastic model describing the porcine ZP reaction forces generated by nanoindentations at different probe rates is developed and verified in this study. Visco-hyperelastic parameters of porcine ZP membranes are determined by means of a hybrid characterization framework combining atomic force microscopy nanoindentation measurements, nonlinear finite-element analysis and nonlinear optimization. Remarkably, it is possible to separate the contributions of hyperelastic and viscous terms to ZP mechanical response and evaluate the error made in the determination of ZP mechanical properties if viscous effects were not considered.

show abstract

supporting

confidence: 68%

Section: Discussionmentioning

confidence: 98%

A hybrid characterization framework to determine the visco-hyperelastic properties of a porcine zona pellucida

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The ability to fertilise seems to be strictly connected with progressive motility, which is dependent on velocity parameters (VAP, VSL, and VCL) and also on ALH and BCF. These values define movement of spermatozoa and its ability to pass through cervical mucus and penetrate the zona pelucida (7,31). This was reported in humans where VCL and BCF were higher for fertile men (9).…”

Section: Discussionmentioning

confidence: 85%

Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

Domosławska

Zduńczyk

Niżański

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

Thirty clinically healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group (15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

show abstract

“…These parameters are probably important for the progression of spermatozoa into cervical mucus and the penetration of zona pellucida of oocytes (26). Fertilisation rates of human oocytes in vitro have been shown to correlate positively with sperm velocity (1,24). In bovine, sperm velocity is highly correlated with the 59 d non-return rate (2).…”

Section: Discussionmentioning

confidence: 99%

Assessment of semen quality in infertile dogs using computer-assisted sperm analysis by the Hamilton-Thorne Semen Analyser

Domosławska

Zduńczyk

Niżański

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

Semen quality parameters of infertile and fertile dogs were compared. Sperm concentration and semen motility parameters were measured by the Hamilton-Thorne Semen Analyser IVOS 12.3. The spermatozoal morphology and the percentage of live spermatozoa were examined microscopically. Forty-six dogs of various breeds were examined. Twenty dogs had a conception failure within last year. These dogs had a history of prior normal fertility. Twenty six fertile dogs served as control. All animals underwent a clinical examination as well as ultrasonography. Sperm concentration was significantly lower in infertile dogs than in fertile dogs. For most determined motility parameters (MOT, PMOT, VAP, VSL, VCL, BCF, RAPID, STATIC) significant differences between infertile and fertile dogs were found. The percentage of spermatozoa with normal morphology also significantly differed between both groups. Ultrasonography of the prostate gland and testes revealed no pathological conditions. The testicular degeneration was assumed to be a possible cause of infertility in these dogs. The present study showed that the most sperm CASA motility parameters were significantly lower in infertile dogs in comparison to the fertile ones, and confirmed the usefulness of the Hamilton-Thorne Semen Analyser for a quick and objective analysis of sperm concentration and motility in dogs.

show abstract

Sperm velocity, strict morphology and the hypo-osmotic swelling test as predictors of the sperm fertilization potential: experience from the IVF model.

Cited by 13 publications

References 0 publications

A hybrid characterization framework to determine the visco-hyperelastic properties of a porcine zona pellucida

A hybrid characterization framework to determine the visco-hyperelastic properties of a porcine zona pellucida

Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

Assessment of semen quality in infertile dogs using computer-assisted sperm analysis by the Hamilton-Thorne Semen Analyser

Contact Info

Product

Resources

About