Sperm transport in the cow : peri-ovulatory redistribution of viable cells within the oviduct

Hunter, R. H. F.; Wilmut, Ian

doi:10.1051/rnd:19840508

Cited by 202 publications

(114 citation statements)

References 15 publications

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“…Moreover, a 70% incidence of fertilization followed intraperitoneal insemination of small volumes of boar semen shortly before ovulation (Hunter, 1978), so a continuous pre-ovulatory progression of spermatozoa up and out of the control oviduct in the present work might have been expected to lead to some fertilizations in the transected oviduct. Taking these observations together, and also the results of comparable surgical studies in sheep (Hunter & Nichol, 1983) and cows (Hunter & Wilmut, 1984), the evidence strongly favours the concept of pre-ovulatory sequestering of viable spermatozoa in the caudal region of the oviduct isthmus. A similar situation occurs in the oviducts of rabbits (Harper, 1973a, b ;Overstreet, Cooper & Katz, 1978 ; and the golden hamster (Yanagimachi & Chang, 1963;Battalia & Yanagimachi, 1979), although Harper (1973a) suggests that it is the "products of ovulation", the eggs, their investments and/or the follicular fluid, entering the oviducts that leads to movement of spermatozoa towards the site of fertilization.…”

Section: Discussionmentioning

confidence: 86%

Pre-ovulatory arrest and peri-ovulatory redistribution of competent spermatozoa in the isthmus of the pig oviduct

Hunter¹

1984

Reproduction

154

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Summary. Using the surgical approach of post-coital ligation and transection of the distal oviduct at different times relative to ovulation, together with subsequent recovery of the eggs, gilts mated at the onset of oestrus were studied for progression of viable spermatozoa within the isthmus. Results are derived from 76 animals and examination of 1047 eggs.Transection of the isthmus 1\ m=. \ 5\ p=n-\ 2\ m=. \ 0cm proximal to the utero-tubal junction at intervals from 3 to 36 h after mating prevented fertilization in 269 of 270 eggs, whereas 98% of 223 eggs were fertilized in the control oviducts. Transection at 38 h ( pr e\ x=r eq-\ ovulatory), 40 h (peri-ovulatory) and 42\p=n-\44 h (post-ovulatory) after mating yielded, respectively, 5%, 40% and 100% fertilization. The mean number of spermatozoa associated with the zona pellucida increased in a parallel manner. These results, and those obtained with ligatures placed closer to the site of fertilization just after ovulation, indicate a pre-ovulatory arrest of viable spermatozoa in the caudal region of the isthmus for 36 h or more followed by an active ad-ovarian redistribution.

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Section: Discussionmentioning

confidence: 86%

Pre-ovulatory arrest and peri-ovulatory redistribution of competent spermatozoa in the isthmus of the pig oviduct

Hunter¹

1984

Reproduction

154

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“…Du Mesnil du Buisson and Dauzier [5] had suspected that either the utero-tubal junction or the isthmus might act as a sperm reservoir, and by surgical and histological studies the latter was shown to be true in pigs [6], sheep [7] and cows [8,9]. We termed the caudal portion of the isthmus the functional sperm reservoir [10], for it was from this region of the tract that spermatozoa involved in the events of fertilisation were activated and released close to the time of ovulation.…”

Section: Functional Sperm Reservoirmentioning

confidence: 99%

The Fallopian tubes in domestic mammals: how vital is their physiological activity?

Hunter¹

2005

Reprod. Nutr. Dev.

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-Set in an historical perspective, this essay examines diverse physiological aspects of Fallopian tube function in domestic animals and man. Microsurgical experiments are described that established the role of the isthmus in imposing a sperm gradient up to the site of fertilisation. Resection of the isthmus followed by reanastomosis of the remaining portions of the tube generated a high incidence of polyspermy in mated animals. Scanning electron microscopy and surgical studies revealed that spermatozoa were arrested and stored in the caudal portion of the isthmus before ovulation, the so-called functional sperm reservoir. There were specific adhesion contacts between the sperm head and endosalpingeal microvilli or cilia. Further experiments indicated that very large numbers of competent spermatozoa could be released from pre-ovulatory binding by microinjections of a solution of progesterone in oil under the serosal layer of the tube: when suitably timed, such treatment led to a high incidence of polyspermic fertilisation. Avid sperm binding in the caudal isthmus before ovulation prevents myosalpingeal activity leading to abnormal fertilisation, as might occur with multiple mating. Temperatures in the reproductive system were assessed and the caudal isthmus was found to be cooler than the ampulla during the pre-ovulatory phase of sperm storage. Finally, the existence of fluid microenvironments within the Fallopian tubes was reported, and the role of suspended cumulus-corona cells in amplifying signals from the zygote examined. An impact of Fallopian tube fluids on embryonic gene expression was also considered -an influence that may be further imposed if such fluids have access to the uterine lumen. tubal anastomosis / isthmus / sperm gradient / fertilisation / polyspermy / microenvironments / temperature

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“…The isthmus part is described as functioning as a sperm reservoirsperm adhere to the epithelium and at the time of ovulation they are released (Hunter & Wilmut 1984) to ensure the perfect timing of fertilisation. In this way, the oviduct provides the microenvironment for sperm capacitation, fertilisation, and early embryonic development (Buhi 2002).…”

Section: Introductionmentioning

confidence: 99%

Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle

Bauersachs¹,

Rehfeld²,

Ulbrich³

et al. 2004

Journal of Molecular Endocrinology

116

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The oviduct epithelium undergoes marked morphological and functional changes during the oestrous cycle. To study these changes at the level of the transcriptome we did a systematic gene expression analysis of bovine oviduct epithelial cells at oestrus and dioestrus using a combination of subtracted cDNA libraries and cDNA array hybridisation. A total of 3072 cDNA clones of two subtracted libraries were analysed by array hybridisation with cDNA probes derived from six cyclic heifers, three of them slaughtered at oestrus and three at dioestrus. Sequencing of cDNAs showing significant differences in their expression levels revealed 77 different cDNAs. Thirty-seven were expressed at a higher level at oestrus, for the other 40 genes expression levels were higher at dioestrus. The identified genes represented a variety of functional classes. During oestrus especially genes involved in the regulation of protein secretion and protein modification, and mRNAs of secreted proteins, were up-regulated, whereas during dioestrus particularly transcripts of genes involved in transcription regulation showed a slight up-regulation. The concentrations of seven selected transcripts were quantified by real-time RT-PCR to validate the cDNA array hybridisation data. For all seven transcripts, RT-PCR results were in excellent correlation (r>0·92) with the results obtained by array hybridisation. Our study is the first to analyse changes in gene expression profiles of bovine oviduct epithelial cells during different stages of the oestrous cycle, providing a starting point for the clarification of the key transcriptome changes in these cells.

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Sperm transport in the cow : peri-ovulatory redistribution of viable cells within the oviduct

Abstract: Introduction.

Cited by 202 publications

References 15 publications

Pre-ovulatory arrest and peri-ovulatory redistribution of competent spermatozoa in the isthmus of the pig oviduct

Pre-ovulatory arrest and peri-ovulatory redistribution of competent spermatozoa in the isthmus of the pig oviduct

The Fallopian tubes in domestic mammals: how vital is their physiological activity?

Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle

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