Sperm swim-up techniques and DNA fragmentation

Younglai, Edward V.; Holt, David W.; Brown, Paula R.; Jurisicova, Andrea; Casper, Robert F.

doi:10.1093/humrep/16.9.1950

Cited by 130 publications

(88 citation statements)

References 21 publications

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“…Several studies have investigated the efficacy of these methods with regards to the recovery of reduced percentage of sperm cells with fragmented DNA, however the results are still controversial [30,31,36,37]. While one study showed that the DGC is more effective than SUP in reducing the percentage of sperm with DNA damage [25], another one, in contrast, demonstrated that SUP recovers lower percentage of sperm with fragmented DNA as compared to DGC [37].…”

Section: Discussionmentioning

confidence: 99%

Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques

Borges

Setti

Vingris

et al. 2013

J Assist Reprod Genet

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Purpose To compare the results of intracytoplasmic morphologically selected sperm injection (IMSI) between cycles in which the swim-up (SUP) or the density gradient centrifugation (DGC) techniques were used for sperm preparation. Methods We evaluated 70 IMSI cycles performed in women with age ≤ 37 years, undergoing IMSI as result of male factor. The couples were divided into two groups: DGC group (n=26) and SUP group (n=44). The groups were compared with regard to IMSI outcomes. Results There were no significant differences between SUP and DGC groups regarding the number of follicles, oocytes, mature oocytes, oocyte yield and mature oocyte rate. Fertilization rate and high-quality embryos rate on day 5 of development were similar between SUP and DGC groups. Implantation, pregnancy and miscarriage rates were not statistically different between SUP and DGC groups (28.8 vs 33.3 %, 46.2 vs 57.1 % and 8.3 vs 4.2 %, respectively). Conclusions Both the SUP and the DGC techniques recover improved sperm fractions and result in similar IMSI outcomes. Further randomized trials analyzing both the quality of sperm through MSOME and the IMSI outcomes are needed to elucidate the role of sperm preparation techniques and morphology on IMSI outcomes.

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Section: Discussionmentioning

confidence: 99%

Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques

Borges

Setti

Vingris

et al. 2013

J Assist Reprod Genet

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show abstract

“…Over the past years, the comparative studies on the sperm preparation methods have essentially investigated the outcomes such as the recovery rates and the conventional semen parameters [3][4][5]. And more recently, researchers have focused on the evaluation of the molecular parameters such as sperm DNA damage or apoptosis for the comparison of these different separation methods [6][7][8][9][10][11][12]. Spano et al, [7] in 1999, have been shown that the swim-up sperm separation may improve some of the sperm chromatin structure assay-related parameters.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the DNA Fragmentation and the Sperm Parameters after Processing by the Density Gradient and the Swim up Methods

Amiri¹

2012

JCDR

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Introduction:The swim up and the density-gradient centrifugation are the two main techniques which are used to separate the viable motile sperm fraction in the assisted reproductive technology. However, there are several published studies about these methods, but there is no sufficient evidence for recommending the superiority of one of them. This study was designed to study the efficiency of the swim-up and the density gradient techniques to recover the spermatozoa with a high degree of motility, a normal morphology and a low level of DNA fragmentation. Material and Methods:A total of 35 semen samples were included in the study. The semen samples were collected, one part of the semen was spread on a slide and the remainder was prepared by using the swim-up or the density gradient techniques. The recovered spermatozoa were evaluated for concentration, motility, and normal morphology. A comet assay was carried out to assay the DNA fragmentation in all the samples.Results: There were significant differences in the sperm parameters between the density gradient and the swim up techniques. Also, the swim-up technique showed a significantly higher level of DNA fragmentation as compare to the density gradient technique. Conclusion:The results of this study demonstrated several benefits of the gradients method in the separation of normal and motile spermatozoa with healthy DNA, in comparison to the swim up method.

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“…[4][5][6][7][8][9] To date, no consensus has been achieved regarding the effect of these techniques on the DNA integrity of sperm. Several investigations have shown that sperm preparation techniques considerably reduce the level of DNA damage present in a semen sample, [4][5][6][7] while other studies have reported that these methods do not recover spermatozoa with lower incidence of DNA damage. 8 Some groups have reported that the DGC technique appreciably reduces the percentage of sperm with DNA damage, whereas the SUP method shows no significant improvement.…”

Section: Introductionmentioning

confidence: 99%

“…5,26 In the case of the SUP approach, most studies agree that this technique allows the efficient elimination of spermatozoa with high levels of DNA damage. [4][5][6] Nevertheless, none of these studies makes any distinction between the types of DNA damage present in the spermatozoa, either before or after sample processing. Therefore, the results presented in this study are novel.…”

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confidence: 99%

The ability of sperm selection techniques to remove single- or double-strand DNA damage

Enciso¹,

Iglesias²,

Galán³

et al. 2011

Asian J Androl

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A wide variety of techniques for the preparation of sperm are currently available, of which the most commonly employed are densitygradient centrifugation (DGC) and swim-up (SUP). To date, these methods appear to be effective in selecting functional sperm for assisted reproduction techniques (ART), but they may have negative effects on sperm DNA. In this study, the ability of these semen processing techniques to eliminate spermatozoa containing single-and double-strand DNA damage was assessed by the two-tailed comet assay and the sperm chromatin dispersion test in 157 semen samples from patients seeking assisted reproduction treatment. Our results indicated that SUP and DGC are equally efficient in eliminating spermatozoa containing double-strand DNA damage and sperm with highly damaged (degraded) DNA, as characterized by the presence of both single-and double-strand DNA breaks. However, DGC is more efficient than SUP in selecting spermatozoa that are free from single-strand DNA damage. Future studies should characterise the importance of the various types of DNA damage and examine the sperm processing protocols used in each laboratory to determine their ability to eliminate DNA damage and hence, prevent the potential transmission of genetic mutations via ART.

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Sperm swim-up techniques and DNA fragmentation

Abstract: We conclude that our normal swim-up technique caused no more DNA damage to spermatozoa from normal semen samples than a direct swim-up technique that involved no centrifugation step.

Cited by 130 publications

References 21 publications

Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques

Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques

Comparison of the DNA Fragmentation and the Sperm Parameters after Processing by the Density Gradient and the Swim up Methods

The ability of sperm selection techniques to remove single- or double-strand DNA damage

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