Sperm solute carrier family 9 regulator 1 is correlated with boar fertility

Kim, Ki-Uk; Pang, Won-Ki; Kang, Saehan; Ryu, Do-Yeal; Song, Won-Hee; Rahman, Saidur; Kwon, Woo-Sung; Pang, Myung-Geol

doi:10.1016/j.theriogenology.2018.12.023

Cited by 19 publications

(19 citation statements)

References 41 publications

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“…Transcriptional profile of specific candidate genes was linked with conventional semen physical and biochemical characteristics predicted male fertility in different farm animal species (Kadivar et al, 2016;Kim et al, 2019). The implication of sperm related genes in fertility was suggested by the involvement of sperm transcripts in different crucial reproductive processes like spermatogenesis, sperm motility (Bissonnette et al, 2009), fertilizing ability and subsequent embryonic development (Boerke et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Profile and Enzymatic Activities of Frozen Buck Sperm Supplemented with Melatonin in Cold and Hot Temperatures.

Dessouki¹,

Ashour²,

El-Gayar³

et al. 2020

jwpr

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This study was carried out to improve the freezability of buck semen using two different types of cryoprotectants supplemented with melatonin as antioxidant in cold and hot temperature of breeding season. Ejaculates from four mature Egyptian baladi bucks were pooled after collection. Semen was extended with Tris-fructose-citric containing egg yolk using glycerol and dimethyl sulfoxide supplemented with two doses of melatonin (10-6 M and 10-3 M) in addition to control group. Types of motility as well as velocity, enzymatic activity and expression profile of selected genes were measured. The results revealed that the progressive motility percentage was significantly higher in samples supplemented with low dose of melatonin (10-6 M) compared to high dose (10-3 M) in glycerol (74.4 versus 64.4) and Dimethyl Sulfoxide (DMSO) based extender (35.5 versus 32.9) in cold temperature. The same trend was found in samples cryopreserved with glycerol (75.1 versus 53.5) and DMSO (32.1 versus 22) in hot temperature. The results also demonstrated that CASA parameters (VAP and VCL) were significantly increased in low compared to high melatonin dose in glycerol based extender during cold and hot temperature. The activity of total antioxidant capacity (TAC) was significantly higher in samples supplemented with low (0.49 mM/L) than high melatonin dose (0.16 mM/L) in DMSO extender. CPT2, ATP5F1A and SOD2 genes were up regulated in glycerol based extender groups in cold temperature compared to other groups of this study. On the other hand, NFE2L2 gene was upregulated in groups cryopreserved with DMSO in hot temperature compared with all other experimental groups. Therefore, it could be concluded that the glycerol based extender in cold season supplemented with low dose of melatonin improved semen quality, antioxidant defense capacity and transcriptional profile, which may maintain the post-thaw fertilizing ability of buck semen.

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Section: Introductionmentioning

confidence: 99%

Gene Expression Profile and Enzymatic Activities of Frozen Buck Sperm Supplemented with Melatonin in Cold and Hot Temperatures.

Dessouki¹,

Ashour²,

El-Gayar³

et al. 2020

jwpr

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“…mTCM 199 medium) (Sigma-Aldrich, St. Louis, MO, USA) to discard seminal plasma and dead spermatozoa. After the Percoll gradient wash, live spermatozoa were cultured in modified tissue culture medium 199 (mTCM 199; containing 0.91 mM sodium pyruvate, 3.05 mM d-glucose, 2.92 mM calcium lactate, and 2.2 g/L sodium bicarbonate; Sigma-Aldrich) and incubated for 30 min at 37 °C under 5% atmospheric CO 2 (14,23). To evaluate sperm functional parameters between the two groups, sperm motility, motion kinematics, sperm capacitation status, and mRNA and protein levels of Prdx4 were evaluated.…”

Section: Animal and Semen Collectionmentioning

confidence: 99%

“…H33258/CTC assessment was conducted to evaluate the capacitation/acrosome status of spermatozoa (2,23). Briefly, 135 μL of each sample was incubated with 15 μL of H33258 solution for 10 min at room temperature.…”

Section: Combined H33258/chlortetracycline Fluorescence (H33258/ctc) mentioning

confidence: 99%

“…We randomly selected 20 Yorkshire boars with unknown fertility from the Grand-Grandparents Farm to evaluate the correlation between litter size and sperm parameters or PRDX expression levels in individual samples. Selected semen was diluted to 30 × 10 6 sperm cells/100 mL with Beltsville Thawing Solution for AI (22,23). Diluted boar semen samples were inseminated by an experienced artificial inseminator into sows (n = 568).…”

Section: Artificial Insemination (Ai)mentioning

confidence: 99%

“…To determine the accuracy of predicting boar fertility based on the litter size, we evaluated the following five major parameters during the screening test: sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall accuracy, which were determined according to the cut-off value associated with receiver operating curve (ROC) analysis as described previously (23,27,29,30). Briefly, cut-off value was decided by the predicted probability that the individual semen appertained to [litter size ≥ 12] according to the marker(s) in logistic regression.…”

Section: Quality Assessment Of Markersmentioning

confidence: 99%

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Peroxiredoxin 4 as potential fertility marker in boars

Ryu

Pang

Rahman

et al. 2020

Preprint

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Background: High levels of reactive oxygen species are toxic to spermatozoa as they induce DNA damage, which leads to male infertility. Therefore, reduction of oxidative stress using reducing agents, such as antioxidant enzymes, is required. Peroxiredoxins (Prdxs) are known to play a critical role in the regulation of male fertility as antioxidant enzymes. Although several studies have suggested a close association between Prdxs and male fertility, few studies have explored the efficacy of Prdxs to predict fertility. Therefore, the current study was designed to discover the most sensitive biomarkers among the Prdxs with six isoforms, and furthermore to determine whether Prdxs are more suitable fertility markers for boar spermatozoa compared to conventional semen analysis.Results: The mRNA levels of PRDXs and several sperm parameters were examined in spermatozoa collected from 20 boars with different litter sizes. Our study showed that there was a significant positive correlation between the litter size and the levels of PRDX 4 among all isoforms in spermatozoa. Subsequently, a regression analysis using a combination of markers was conducted to increase efficacy for fertility prediction. According to the data, PRDX4 had the highest efficacy compared to other combination models. The prediction accuracy of male fertility was further evaluated through receiver operating characteristic curve analysis, which showed that PRDX 4 could predict the litter size with a high overall accuracy of 95%.Conclusions: As fertility biomarkers, genomic markers might be more accurate for predicting male fertility compared to conventional semen analysis. Our findings indicate that PRDX 4 might be a potential biomarker for diagnosing male fertility and subsequently improving reproductive efficacy in boars.

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Sperm capacitation as a predictor of boar fertility

Keller

Kerns

2023

Molecular Reproduction Devel

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Prediction of a boar's fertility level has great economic importance for sow herds. After standard sperm morphology and motility metrics are met, approximately 25% of boars have less than 80% conception rates. Due in part to the many factors involved in the fertilization process, a multifactorial model incorporating multiple relevant sperm physiology factors will likely lead to increased understanding of boar fertility. Here we review the current literature on boar sperm capacitation as a predictor of boar fertility. While limited, several studies have provided correlations between the percentage of sperm in an ejaculate that are capable of undergoing sperm capacitation in a chemically defined media and artificial insemination field fertility as well as proteome and other methods. Work summarized here underscores the need for further understanding of boar fertility.

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Sperm solute carrier family 9 regulator 1 is correlated with boar fertility

Cited by 19 publications

References 41 publications

Gene Expression Profile and Enzymatic Activities of Frozen Buck Sperm Supplemented with Melatonin in Cold and Hot Temperatures.

Gene Expression Profile and Enzymatic Activities of Frozen Buck Sperm Supplemented with Melatonin in Cold and Hot Temperatures.

Peroxiredoxin 4 as potential fertility marker in boars

Sperm capacitation as a predictor of boar fertility

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