Sperm DNA fragmentation on the day of fertilisation is not associated with assisted reproductive technique outcome independently of gamete quality

Ten, Jorge; Guerrero, Jaime; Linares, Ángel; Rodríguez-Arnedo, Adoración; Morales, Ruth; Lledó, Belén; Llácer, J.; Bernabéu, Rafael

doi:10.1080/14647273.2021.1877364

Cited by 7 publications

(5 citation statements)

References 47 publications

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“…Sperm DNA fragmentation refers to any break in one or both of the strands of DNA in the head of a sperm [ 20 ]. Breaks in the DNA may play a crucial role in gamete function and, as a consequence may affect fertilization, embryo development, and the reproductive outcome [ 9 , 10 , 11 , 12 , 13 ]. Aspects of sperm function that can be disrupted include motility and sperm–zona recognition.…”

Section: Discussionmentioning

confidence: 99%

“…The impact DNA fragmentation may have on the success of assisted reproduction cycles highly depends on the balance between the extent of the DNA breaks, and the ability of the oocyte to repair this damage. This diversity in sperm DNA damage and the repair capacity of the oocyte may explain why some fragmented sperm retain their fertilizing ability [ 9 , 10 , 11 , 12 , 13 , 20 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Sperm DNA fragmentation has not yet been shown to be a good predictor of positive HCG, clinical pregnancy, miscarriage, and live births during ICSI or IVF cycles [ 12 ]. A retrospective analysis of clinical outcomes in vitrified–warmed single-blastocyst transfer cycles, in relation to sperm DNA fragmentation, revealed no straight correlation between a positive hCG rate, a clinical pregnancy rate, and first trimester miscarriage, and DFI levels, suggesting that ART outcomes are not affected by sperm DNA fragmentation independently of the gamete quality [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

Chatzimeletiou,

Fleva,

Nikolopoulos

et al. 2023

Medicina

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Background and Objectives: Sperm DNA fragmentation refers to any break in one or both of the strands of DNA in the head of a sperm. The most widely used methodologies for assessing sperm DNA fragmentation are the sperm chromatin structure assay (SCSA), the sperm chromatin dispersion assay (SCD), the single-cell gel electrophoresis assay (SCGE–comet), and the terminal-deoxynucleotidyl-transferase (TdT)-mediated dUTP nick end labelling (TUNEL) assay. The aim of this study was to compare the efficiency and sensitivity of the analysis of sperm DNA fragmentation using TUNEL via fluorescence microscopy, and flow cytometry. Materials and Methods: Semen samples were collected and analyzed for standard characteristics using light microscopy, and for sperm DNA fragmentation using both TUNEL via fluorescence microscopy, and flow cytometry. Results: There were no significant differences in the values of the sperm DNA fragmentation index (DFI) obtained when the analysis was performed using TUNEL or flow cytometry (p = 0.543). Spearman’s correlation analysis revealed a significant negative correlation between sperm motility (%) and sperm DNA fragmentation (p < 0.01), as well as between sperm concentration and sperm DNA fragmentation (p < 0.05). The Mann–Whitney U test showed no significant difference in the DFI among couples with repeated implantation failure (RIF) and miscarriages (p = 0.352). Conclusions: Both methods (TUNEL via fluorescence microscopy, and flow cytometry) have a high efficiency and sensitivity in accurately detecting sperm DNA fragmentation, and can be effectively used to assess male fertility.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

Chatzimeletiou,

Fleva,

Nikolopoulos

et al. 2023

Medicina

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“…biochemical and molecular functions of sperm, including measurement of seminal ROS [24] and SDF [25], which may be more predictive of fertility potential compared to standard semen parameters [26]. Furthermore, these biomarkers have been used to stratify patients who may experience success with assisted reproductive techniques (ART) [26][27][28][29]. Sperm DNA is highly organised and the degree of chromatin organisation can affect epididymal and embryo development [30].…”

Section: The Role Of Molecular Testingmentioning

confidence: 99%

European Association of Urology Guidelines on Male Sexual and Reproductive Health: 2021 Update on Male Infertility

et al. 2021

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“…Detailed evaluation of embryo development can be one of the appropriate strategies to determine whether sperm DNA fragmentation can be considered as one of the biomarkers of sperm quality ( 21 ). Although studies indicated that there can be possible negative impact of sperm DNA fragmentation on embryological parameters and implantation potential ( 22 , 23 ), others did not report such an impact of sperm DNA damage on early embryonic development and clinical pregnancy rate ( 24 , 25 ). Recently, Ribas-Maynou et al showed a negative but insignificant trend of implantation and live birth rates in samples with high DFI ( 26 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Efficiency of Magnetic-Activated Cell Sorting (MACS) and Physiological Intracytoplasmic Sperm Injection (PICSI) for Sperm Selection in Cases with Unexplained Infertility

Ahmadi¹,

Sobhani²,

Khalili³

et al. 2022

JRI

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Background: The cases with unexplained infertility may have an abnormality in their sperm chromatin structure. Sperm selection methods can be used to separate sperm with low DNA fragmentation. The purpose of this study was to compare the efficacy of physiological intracytoplasmic sperm injection (PICSI) with magnetic-activated cell sorting (MACS) in assisted reproductive techniques in cases with unexplained infertility. Methods: The semen samples were collected from couples with unexplained infertility. After semen analysis and sperm DNA fragmentation (SDF) evaluations, samples were prepared with swim-up method. The rates of SDF in different fractions including raw semen (n=20), swim-up (n=20), only motile sperm after swim-up (swim-up selection) (n=20), MACS sperm selection (n=20), only motile sperm after MACS (MACS selection) (n=20), and PICSI sperm selection (n=16) were evaluated. Also, the main sperm characteristics and fine morphology of sperm suspension after MACS were assessed. Statistical analysis was performed using GraphPad Prism. The p<0.05 was considered statistically significant. Results: DNA fragmentation index (DFI) values in PICSI and MACS groups were significantly reduced as compared to the swim-up group. The rate of this reduction was more pronounced in MACS (58.20±13.02) than PICSI (36.57±15.52) group. Also, our results showed that MACS resulted in decreased sperm motility, with no alteration in their fine morphology. Conclusion: MACS was found to be more efficient in reduction of SDF rates than PICSI. However, none of the sperm selection techniques can not totally eliminated the spermatozoa with DNA fragmentation in the final sperm sample.

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Sperm DNA fragmentation on the day of fertilisation is not associated with assisted reproductive technique outcome independently of gamete quality

Cited by 7 publications

References 47 publications

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

European Association of Urology Guidelines on Male Sexual and Reproductive Health: 2021 Update on Male Infertility

Comparison of the Efficiency of Magnetic-Activated Cell Sorting (MACS) and Physiological Intracytoplasmic Sperm Injection (PICSI) for Sperm Selection in Cases with Unexplained Infertility

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