2004
DOI: 10.1016/j.cryobiol.2004.02.004
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Abstract: Sperm cryopreservation for fishes with internal fertilization is essentially unexplored although many species of these fishes are valuable biomedical research models. To explore methods for sperm cryopreservation within the live-bearing genus Xiphophorus, this study used X. helleri to evaluate the effects of cryoprotectant, osmotic pressure, cooling rate, equilibration time, and sperm-to-extender ratio. Sperm motility and survival duration after thawing showed significant differences among different cryoprotec… Show more

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Cited by 45 publications
(62 citation statements)
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“…31 In addition, sperm concentration was estimated and no attempts were made to adjust the concentration to an established value. 30 In the present study, we tested the effect of nine cryoprotectants at six concentrations (5%-30%) and at two exposure temperatures on sperm at one standardized concentration (1 · 10 8 cells/mL). We evaluated motility immediately (within 10 s) after the addition of the cryoprotectant, and at 7 time intervals (as long as 60 min).…”
Section: Identifying Suitable Cryoprotectantsmentioning
confidence: 99%
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“…31 In addition, sperm concentration was estimated and no attempts were made to adjust the concentration to an established value. 30 In the present study, we tested the effect of nine cryoprotectants at six concentrations (5%-30%) and at two exposure temperatures on sperm at one standardized concentration (1 · 10 8 cells/mL). We evaluated motility immediately (within 10 s) after the addition of the cryoprotectant, and at 7 time intervals (as long as 60 min).…”
Section: Identifying Suitable Cryoprotectantsmentioning
confidence: 99%
“…It has been suggested that as the concentration of Gly increases, the post-thaw motility of green swordtail sperm increases. 30 In addition to Gly, we decided to use DMSO because in previous studies it was used for conventional cryopreservation in green swordtail. 13,30 Post-thaw motilities in a previous study of conventional cryopreservation using 20% DMSO were around 15%, whereas they were 50% for 20% Gly.…”
Section: Evaluating Vitrification Solutionsmentioning
confidence: 99%
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