Sperm characteristics, peroxidation lipid and antioxidant enzyme activity changes in milt of Brycon orbignyanus cryopreserved with melatonin in different freezing curves

Palhares, Priscila Cotta; Assis, Isadora de Lima; Machado, Gilmara Junqueira; Freitas, Renata Maria Pereira de; Freitas, Mariella Bontempo; Paula, Daniella Aparecida de Jesús; Carneiro, William Franco; Motta, Naiara Cristina; Murgas, Luís David Solis

doi:10.1016/j.theriogenology.2021.09.013

Cited by 15 publications

(9 citation statements)

References 37 publications

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“…This is consistent with the results of a previous study by Palhares et al . [ 21 ] that showed that melatonin protects against oxidative stress and consequently maintains sperm motility. Melatonin has a potential cryoprotective effect on mitochondrial functioning, which is essential for generating energy from intracellular adenosine triphosphate reserves, thereby enhancing sperm motility [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Widyastuti,

Prastowo,

Jaswandi

et al. 2024

Vet World

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Background and Aim: Semen storage is an important reproductive method used in artificial livestock breeding. However, oxidative stress during storage reduces the quality of sperm. Melatonin supplementation in semen storage medium has not been well studied, but it has been shown to protect cells from oxidative stress. Therefore, this study aimed to determine the effect of melatonin supplementation on sperm quality parameters and antioxidant gene expression levels in semen extenders during cold storage. Materials and Methods: Semen extenders with melatonin concentrations of 0 (control), 0.1, 0.2, and 0.3 mM were added as treatment. The treated semen was then stored at 5°C for 72 h using a cold storage method, and quality parameters, including percentage of progressive motility, membrane integrity, intact acrosome, and DNA integrity, were measured every 24 h. In addition, messenger ribonucleic acid abundance levels of glutathione peroxidase (GPx) and superoxide dismutase (SOD) genes were sampled after 0 and 72 h of cold storage. Results: All observed sperm quality parameters decreased with increasing cold storage time; however, 0.2 mM melatonin demonstrated superior protection of sperm quality during cold storage. Gene expression analysis showed that GPx levels decreased significantly (p < 0.05) after 72 h in semen without melatonin but not in the melatonin-treated groups. A similar trend was also observed in SOD, indicating that exogenous antioxidants effectively protected the sperms. Conclusion: Melatonin supplementation at 0.2 mM in semen extenders during cold storage maintains sperm quality parameters for up to 72 h because melatonin protects sperm from oxidative stress. These findings can be used to improve the semen storage protocol by combining semen extender and antioxidant. Keywords: antioxidant gene expression, melatonin, semen cold storage, sperm quality.

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Section: Discussionmentioning

confidence: 99%

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Widyastuti,

Prastowo,

Jaswandi

et al. 2024

Vet World

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“…Furthermore, the reduced of temperature during chilling processing which preserved at 5 °C or less inflicts sub-lethal damage to spermatozoa, compromising sperm quality and the success of artificial breeding as described by previous studies (Mohamed et al, 2020 andBustani andBaiee 2021). The earlier studies have concluded effect of chilling temperature on the sperm motility in both general and progressive motile of rat (Varisli et al, 2009), bull (Januskauskas et al, 1999), rabbit (Rosato et al, 2011), human (Verheyen et al, 1993) and, ram (Kulaksiz et al, 2012) which illustrated the temperature related in the organization of structural components of the plasma membrane have profound effects upon its properties, associated with changes in permeability, changes in membrane fluidity (Ortega et al, 2019), dis-regulation of intracellular Ca+' influx (Yeste et al, 2018) and changes in enzyme activity (Palhares et al, 2021) finally on the ability of sperm capacitation. From the Figure (1 and 2) the result showed the improved effect of Prunus cerasus (G1) on the sperm motility (general and progressive).…”

Section: Discussionmentioning

confidence: 99%

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

AbdUlAmeer¹,

Zaid²

2022

ijhs

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This study was designed in order to investigate and determine the effect of black current (Prunus cerasus) extract on semen general and progressive motility of the rams. Twelve mature healthy rams was used in this study aged between 2-3 years, kept in the animal farm of the College of Veterinary Medicine, Al-Kufa University. Semen ejaculates was collected using electroejaculator methods once weekly a total of 36 ejaculates were collected among the period of the study. The black current (Prunus cerasus) extract was prepared by using maceration and added to the semen in five doses groups (0.25 gm/ml as G1, 0.5 gm/ml as G2, 1 gm/ml as G3, 2 gm/ml as G4, 4 gm/ml as G5) and the positive control group left without any addition. The treatment samples were evaluated under cooling for five consecutive days, the general and progressive motility were analysis using Computer Assisted Sperm Analysis (CASA). The results of the study clearly indicated that there were a significant differences (P<0.05) in G1 and G2 than other groups in five days of examination, with the preference to the G1 group.

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“…An example of this damage is the formation of intracellular ice crystals, which results in rupture of the plasma membrane due to thermal, mechanical, chemical, and osmotic stress exerted on the cell during freezing (Salmito-Vanderley et al, 2012). In addition, there may be toxicity of the cryoprotectant solution used or sperm sensitivity to reactive oxygen species (ROS) at high concentrations, generating oxidative stress (Palhares et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Cryopreservation of fish semen carries benefits such as the elimination of problems caused by asynchronous gonadal maturity between males and females; simplification of broodstock management at different times; ease in the transport of genetic material between fish farms; conservation of gametes from selected animals in breeding programs; and establishment of hybridization programs during cryopreservation, which reduces the concentration of these antioxidants and favors imbalance, compromising the structural integrity and function of sperm (Silva & Guerra, 2012;Martínez-Páramo et al, 2012). Therefore, to mitigate the damage caused by ROS, substances with antioxidant potential have been added to the semen freezing media of several fish species, including Rhamdia quelen (Xavier et al, 2021), Brycon orbignyanus (Palhares et al, 2021), Prochilodus brevis (Almeida-Monteiro et al, 2017), Cyprinus carpio (Linhares et al, 2017), and C. macropomum (Lopes et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Suplementação do meio criodiluidor com polissacarídeos sulfatados de macroalgas verdes na congelação do sêmen de Colossoma macropomum

et al. 2023

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O estudo teve como objetivo avaliar a adição de polissacarídeos sulfatados (PS) extraídos de duas espécies de macroalgas verdes, Ulva lactuca e Caulerpa racemosa, no meio criodiluidor do sêmen de Colossoma macropomum. Para isso, foram avaliadas quatro concentrações de PS (1,0; 2,0; 3,0 ou 4,0 mg mL-1), de cada macroalga. A coleta de sêmen foi realizada durante o mês de setembro, em Fortaleza, Ceará, Brasil. As amostras de sêmen fresco foram analisadas quanto aos parâmetros de motilidade total dos espermatozoides, velocidade curvilinear (VCL), velocidade em linha reta (VSL), velocidade média do trajeto (VAP), morfologia espermática, integridade de membrana e integridade de DNA. Em seguida, foram criopreservadas em meio de congelação contendo dimetilsulfóxido (DMSO) 10% + glicose 5%, e suplementadas com as diferentes concentrações de PS, tendo ainda um tratamento não suplementado como controle. Após 15 dias, foram descongeladas em banho-maria a 45 ºC por oito segundos, e as mesmas análises do sêmen fresco foram realizadas. Através da análise estatística, os resultados mostraram que não houveram diferenças significativas (p > 0,05) entre as diferentes concentrações de PS testadas para nenhum dos parâmetros avaliados. Já em relação ao controle, não houve diferença nas concentrações (p > 0,05) para a motilidade total, no entanto, para VCL, VSL e VAP, as concentrações de 3,0 e 4,0 mg mL-1 de U. lactuca foram prejudiciais (p < 0,05). O mesmo foi observado em 4,0 mg mL-1 de C. racemosa para VSL e VAP. Para a morfologia, 1,0 e 4,0 mg mL-1 de C. racemosa reduziram os espermatozoides normais (p < 0,05), enquanto para as demais concentrações não houve diferença (p > 0,05). Para todas as concentrações de ambas as macroalgas, a integridade de membrana plasmática foi mantida (p > 0,05). Quanto à integridade do DNA, apenas 4,0 mg mL-1 de U. lactuca foi inferior ao controle (p < 0,05), enquanto as demais concentrações mantiveram o número de espermatozoides com DNA íntegro (p > 0,05). De acordo com os resultados obtidos, concentrações mais elevadas de PS são prejudiciais aos espermatozoides de tambaqui no meio de congelação, enquanto concentrações mais baixas mantiveram parâmetros espermáticos. Estudos posteriores são indicados para melhor avaliar o potencial antioxidante destes polímeros no meio criodiluidor do sêmen de C. macropomum, bem como de outras espécies de peixes.

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Sperm characteristics, peroxidation lipid and antioxidant enzyme activity changes in milt of Brycon orbignyanus cryopreserved with melatonin in different freezing curves

Cited by 15 publications

References 37 publications

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

Suplementação do meio criodiluidor com polissacarídeos sulfatados de macroalgas verdes na congelação do sêmen de Colossoma macropomum

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