Sperm characteristics of Africanized honey bee (
            <i>Apis mellifera</i>
            L.) drones during dry and wet seasons in the Caatinga biome

Morais, Lucas da Silva; Neto, Edgar Rodrigues de Araújo; Silva, Andréia Maria; Marinho, Debora E. L.; Bezerra, Luana Grasiele Pereira; Velarde, Jonatan Mikhail Del Solar; Gramacho, Kátia Peres; Message, Dejair

doi:10.1080/00218839.2022.2113328

Cited by 5 publications

(4 citation statements)

References 51 publications

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“…Drone sperm produced in the spring were somewhat longer and also had longer individual structural elements (19). However, according to Morais et al (36), drone sperm morphometry vary according to rainy and dry seasons. Tarliyah et al (45) and Power et al (42) showed high morphological variation in drone sperm.…”

Section: Discussionmentioning

confidence: 96%

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Identification of honey bee sperm structures following the use of various staining techniques

Banaszewska

Andraszek

2023

Journal of Veterinary Research

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Introduction Bees are currently artificially inseminated on a large scale for breeding and research purposes. The sperm of bees has a complex and varied structure, and determination of specific morphological defects in it is very difficult. Its comprehensive analysis by inspecting morphology and morphometry is an important tool for improving honey bee lines. The staining technique should interfere with the cells as little as possible while clearly showing the boundaries of the head and other elements. In this study, a comparative analysis of the morphometry of sperm was performed with various techniques for staining drone semen. Material and Methods Semen was collected from 150 sexually mature Buckfast bee drones by artificially everting the copulatory organ. The morphology and morphometry of the sperm were assessed on slides prepared by three staining methods according to the protocols described online, using the Sperm Class Analyzer system. The lengths of the acrosome, nucleus, head in total, midpiece, tail without midpiece, tail with midpiece, and entire sperm were measured. Results The most details of the drone sperm structure could be seen when stained with the eosin-nigrosin complex. This method made it possible to identify all structures and revealed the uneven distribution of sperm proteins in different parts of the tail. With the Sperm Stain method fewer details of the sperm structure were recognisable, and the fewest were with SpermBlue. Conclusion The staining method, and thus the chemical reagents used, affect the dimensions of drone sperm. Given the great research potential of modified spermatozoa of insects, a standard for slide preparation for the evaluation of morphological and morphometric semen parameters should be established, as this would facilitate result comparison between laboratories and increase the value of morphological analysis of sperm for predicting and assessing fertility.

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Section: Discussionmentioning

confidence: 96%

“…However, according to Morais et al . ( 36 ), drone sperm morphometry vary according to rainy and dry seasons. Tarliyah et al .…”

Section: Discussionmentioning

confidence: 99%

Identification of honey bee sperm structures following the use of various staining techniques

Banaszewska

Andraszek

2023

Journal of Veterinary Research

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“…Regarding light intensity, solar radiation, CO 2 , atmospheric pressure, and photoperiod those were scarcely represented. In general, these variables were independently analyzed under both experimental (e.g., Butolo et al., 2021; Medina et al., 2018) and empirical approaches (e.g., Gordo & Sanz, 2006; Jaboor et al., 2022), including different combinations of environmental factors (e.g., Morais et al., 2022; Norrström et al., 2021; Rowland et al., 2021). Likewise was the case for their effects, where studies usually analyze the individual effect on selected response variables (e.g., gene expression, visitation rate, appearance date, and survival) and in some cases are considered the interactive effect only between environmental variables (e.g., temperature + precipitation, temperature + photoperiod; e.g., Becsi et al., 2021; Villagomez et al., 2021; Zhang et al., 2022; Norrström et al., 2021; Maluf et al., 2022; Anderson & Maes, 2022) or with other stressors such as pesticides and diseases (e.g., Aldea‐Sánchez et al., 2021; Gonzalez et al., 2022); those studies provide an important insight as the climate impacts can be also exacerbated in different ways by their interaction with other stressors.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, precipitation increase could positively affect reproduction (e.g., increasing sperm number, morphometric, motility, and drone viability; Morais et al., 2022), increase the abundance of beneficial microorganisms (e.g., B. asteroides , L. kunkeei , Lactobacilus sp., and Bartonella sp. ; Castelli et al., 2022), positively affect plant–pollinator interactions (Cruz et al., 2022), increase mitochondrial diversity (Cánovas et al., 2014), reduce Varroa spp.…”

Section: Discussionmentioning

confidence: 99%

Advances and knowledge gaps on climate change impacts on honey bees and beekeeping: A systematic review

Zapata‐Hernández,

Gajardo‐Rojas,

Calderón‐Seguel

et al. 2024

Global Change Biology

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The Western honey bee Apis mellifera is a managed species that provides diverse hive products and contributing to wild plant pollination, as well as being a critical component of crop pollination systems worldwide. High mortality rates have been reported in different continents attributed to different factors, including pesticides, pests, diseases, and lack of floral resources. Furthermore, climate change has been identified as a potential driver negatively impacting pollinators, but it is still unclear how it could affect honey bee populations. In this context, we carried out a systematic review to synthesize the effects of climate change on honey bees and beekeeping activities. A total of 90 articles were identified, providing insight into potential impacts (negative, neutral, and positive) on honey bees and beekeeping. Interest in climate change's impact on honey bees has increased in the last decade, with studies mainly focusing on honey bee individuals, using empirical and experimental approaches, and performed at short‐spatial (<10 km) and temporal (<5 years) scales. Moreover, environmental analyses were mainly based on short‐term data (weather) and concentrated on only a few countries. Environmental variables such as temperature, precipitation, and wind were widely studied and had generalized negative effects on different biological and ecological aspects of honey bees. Food reserves, plant‐pollinator networks, mortality, gene expression, and metabolism were negatively impacted. Knowledge gaps included a lack of studies at the apiary and beekeeper level, a limited number of predictive and perception studies, poor representation of large‐spatial and mid‐term scales, a lack of climate analysis, and a poor understanding of the potential impacts of pests and diseases. Finally, climate change's impacts on global beekeeping are still an emergent issue. This is mainly due to their diverse effects on honey bees and the potential necessity of implementing adaptation measures to sustain this activity under complex environmental scenarios.

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Africanized honeybee (Apis mellifera) semen freezing using Tris-based and Collins extenders

da Silva Morais,

de Araujo Neto,

da Silva

et al. 2023

Trop Anim Health Prod

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Sperm characteristics of Africanized honey bee ( Apis mellifera L.) drones during dry and wet seasons in the Caatinga biome

Cited by 5 publications

References 51 publications

Identification of honey bee sperm structures following the use of various staining techniques

Identification of honey bee sperm structures following the use of various staining techniques

Advances and knowledge gaps on climate change impacts on honey bees and beekeeping: A systematic review

Africanized honeybee (Apis mellifera) semen freezing using Tris-based and Collins extenders

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