Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Grujić, Marica; Dojnov, Biljana; Potočnik, Ivana; Duduk, Bojan; Vujčić, Zoran

doi:10.1016/j.ibiod.2015.04.029

Cited by 69 publications

(35 citation statements)

References 37 publications

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“…The results of SSF showed that the isolate, T. longibrachiatum A-01 produced endoglucanase, exoglucanase, and xylanase at a higher titer. Similar results were reported earlier by Grujić et al [15], when Trichoderma and Aspergillus were used for production of cellulolytic enzymes. Coproduction of cellulase and xylanase by the strain T. longibrachiatum A-01 is highly significant as it helps in achieving complete hydrolysis of lignocellulosic biomass [43,44].…”

Section: Discussionsupporting

confidence: 91%

“…It is reported that cellulase and xylanase are coproduced in Trichoderma spp. due to the fact that induction of both is closely related and that the product of one of the induced enzyme acts as an inducer of another enzyme in the complex [15,45]. Additionally, cellobiase from A. aculeatus C-08 could be used as a supplement during enzymatic saccharification of the lignocellulosic biomass to obtain maximum sugars.…”

Section: Discussionmentioning

confidence: 99%

“…Determination of the total protein produced by fungi can be used to calculate the specific activity of an enzyme for comparing the production capacity of diverse fungal cultures [15]. The high levels of specific activity in the isolate, T. longibrachiatum A-01 and A. aculeatus C-08, indicated that cellulolytic enzyme complex was the most abundant protein in their protein pool.…”

Section: Discussionmentioning

confidence: 99%

“…The cellulosic fraction together with hemicellullose (approximately 57%) in SMS projects it as a potential feedstock for cultivation of cellulolytic fungi. Trichoderma and Aspergillus are the most studied organisms for production of cellulase, xylanase, and amylase at industrial level [15]. These enzymes have crucial importance owing to their application in biorefineries; amylase in the first generation followed by cellulase and hemicellulase in the second generation [16].…”

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confidence: 99%

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Sustainable use of the spent mushroom substrate of Pleurotus florida for production of lignocellulolytic enzymes

et al. 2019

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Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57-74.3% of holocellulose fraction. This study was aimed at utilizing SMS of Pleurotus florida for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of Trichoderma and Aspergillus under solid-state fermentation (SSF). SMS of P.florida extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, Trichoderma longibrachiatum A-01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xylanase (1,550.4 ± 11.6 U/mg) while Aspergillus aculeatus C-08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44%(4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2-, 4.5-, 4.1-, and 5.0-fold as compared with crude extract. The results reveal that SMS from P. florida could be a potential and cost-effective substrate for production of cellulolytic enzymes from T. longibrachiatum A-01 and A. aculeatus C-08. K E Y W O R D Slignocellulolytic enzymes, Pleurotus florida, solid-state fermentation, spent mushroom substrate

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Sustainable use of the spent mushroom substrate of Pleurotus florida for production of lignocellulolytic enzymes

et al. 2019

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“…Mushrooms production continuously increases; China is the biggest producer around the world (Patel, Naraian, & Singh, ). The commercial production in 2012 hit 7,959,979 tons of mushrooms, with China accounting for most of the production (5,150,000 tons), while 1,869,091 tons were harvested in Europe (Grujic, Potocnik, Duduk, & Vujcic, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of pretreatments and drying methods on physico-chemical, sensory characteristics and nutritional value of oyster mushroom

Maray

Mostafa

El-Fakhrany

2017

J Food Process Preserv

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This study was aimed to evaluate the effect of pretreatments and drying methods on the chemical, physico-chemical, sensory characteristics and nutritional value of oyster mushroom. Chemical analysis of oyster mushroom (OM) showed that the values content were increased by many times when calculated on dry weight basis. Nine treatments of dried mushroom powders (DMP) were prepared using three drying methods (hot air drying, vacuum drying, and sun drying). Also, three pretreatments of each method of drying were used (untreated, blanching by steaming without or with steeped in 0.5% citric acid solution for 30 min).The results and statistical analysis revealed that, sun dried oyster mushroom samples (without pretreatments) were found to be the best. The DMP samples resulting of the sun drying had a highest physico-chemical properties, sensory evaluation scores, and contents of the mineral elements and amino acids as compared to other pretreatments and drying methods. Practical applicationsFresh mushrooms have high water content, high enzymatic activity and hence are highly perishable. Thus, in this study nine treatments of dried mushroom powders (DMP) were prepared using three drying methods (hot air drying, vacuum drying and sun drying).Also, three pretreatments of each method of drying were used (untreated, blanching by steaming without or with steeped in citric acid).The DMP samples (untreated) resulting of the sun drying were better than other pretreatments and drying methods regarding quality attributes. Fresh mushrooms are highly perishable because they contain about 87 to 95% water. Due to their high moisture content they cannot be stored for more than 24 hr at ambient conditions. Hence, they need to be preserved by some method. Efficient preservation methods may extend shelf life and diversify the product for consumers. Preservation may also be useful if mushrooms are to be used as an ingredient for the production of other foods like dehy- The purpose of this study is to compare between the different pretreatments and drying methods on quality properties of oyster mushroom powders to identify the best drying method for DMP product. | M A TE RI A L S A ND M E TH ODS | MaterialsThe fresh oyster mushroom (Pleurotus ostreatus) used in the present study was obtained from one of companies for mushroom production, Giza Governorate, Egypt. | MethodsFresh fruiting bodies of oyster mushrooms, cleaned with water, cut into slices (about 3 mm thickness) by knife, then followed with pretreatments and drying methods. Table 1 show different methods and pretreatments of drying for oyster mushroom. | Drying of oyster mushroomMushroom samples (untreated and treated) were dried using three methods of drying according to the following: Hot air dryingMushroom samples were placed in stainless steel trays, and then dried in an oven drying at 55 8C 6 2 until reaching a constant weight according to Chang and Quimio (1982). Vacuum dryingMushroom samples were placed in stainless steel trays, and then dried in an oven drying und...

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Microbial Cellulolytic‐Based Biofuel Production

Bhatt¹

2021

Bioenergy Research

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Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Cited by 69 publications

References 37 publications

Sustainable use of the spent mushroom substrate of Pleurotus florida for production of lignocellulolytic enzymes

Sustainable use of the spent mushroom substrate of Pleurotus florida for production of lignocellulolytic enzymes

Effect of pretreatments and drying methods on physico-chemical, sensory characteristics and nutritional value of oyster mushroom

Microbial Cellulolytic‐Based Biofuel Production

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