Spectrum of the Ma genes for resistance to Meloidogyne spp. in Myrobalan plum

Lecouls, A.C.; Salesses, G.; Minot, J. C.; Voisin, R; Bonnet, A.; Esmenjaud, Daniel

doi:10.1007/s001220050700

Cited by 45 publications

(50 citation statements)

References 19 publications

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“…Di Vito et al (2002) indicated that resistance of Prunus to root-knot nematodes (Meloidogyne spp.) is controlled by several different genes confirming previous findings (Lecouls et al, 1997;Pinochet, 1997). On the other hand, these positive and negative molecular markers using RAPD-PCR analysis must be confirmed when F 2 and F 3 segregants obtained through bulked segregant analysis technique which is beyond, the scope of this study.…”

Section: Rapd Markerssupporting

confidence: 85%

Molecular Genetic Markers for Root-Knot Nematode Resistance in Some Peach Rootstocks

Ahmed¹,

El-Seoudy²,

Fahmy³

et al. 2012

Egyptian Journal of Genetics and Cytology

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Section: Rapd Markerssupporting

confidence: 85%

Molecular Genetic Markers for Root-Knot Nematode Resistance in Some Peach Rootstocks

Ahmed¹,

El-Seoudy²,

Fahmy³

et al. 2012

Egyptian Journal of Genetics and Cytology

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“…Clones P.2175, P.1079 and P.2980 of the Myrobalan plum proved to be resistant to the population Meloidogyne sp. Florida Lecouls et al 1997;Rubio-Cabetas et al 1999). All three clones carry one dominant allele of a single resistance gene, designated Ma1, Ma2 and Ma3, respectively (Esmenjaud et al 1996b;Rubio-Cabetas et al 1998).…”

Section: Introductionmentioning

confidence: 96%

Location of independent root-knot nematode resistance genes in plum and peach

Claverie¹,

Bosselut²,

Lecouls³

et al. 2003

Theor Appl Genet

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Prunus species express different ranges and levels of resistance to the root-knot nematodes (RKN) Meloidogyne spp. In Myrobalan plum ( Prunus cerasifera), the dominant Ma gene confers a high-level and wide-spectrum resistance to the predominant RKN, Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica and the isolate Meloidogyne sp. Florida which overcomes the resistance of the Amygdalus sources. In Japanese plum ( Prunus salicina), a similar wide-spectrum dominant resistance gene, termed R(jap), has been hypothesized from an intraspecific segregating cross. In peach, two crosses segregating for resistance to both M. incognita and M. arenaria were used to identify single genes that each control both RKN species in the Shalil ( R(Mia557)) and Nemared ( R(MiaNem)) sources. Localisation of these genes was made possible using the RFLP and SSR- saturated reference Prunus map TxE, combined with a BSA approach applied to some of the genes. The Ma1 allele carried by the Myrobalan plum accession P.2175 was localised on the linkage group 7 at an approximate distance of 2 cM from the SSR marker pchgms6. In the Japanese plum accession J.222, the gene R(jap) was mapped at the same position in co-segregation with the SSR markers pchgms6 and CPPCT022. The peach genes R(Mia557) and R(MiaNem), carried by two a priori unrelated resistance sources, were co-localized in a subtelomeric position on linkage group 2. This location was different from the more centromeric position previously proposed by Lu et al. (1999) for the resistance gene Mij to M. incognita and M. javanica in Nemared, near the SSR pchgms1 and the STS EAA/MCAT10. By contrast, R(Mia557) and R(MiaNem) were flanked by STS markers obtained by Yamamoto and Hayashi (2002) for the resistance gene Mia to M. incognita in the Japanese peach source Juseitou. Concordant results for the three independent sources, Shalil, Nemared and Juseitou, suggest that these peach RKN sources share at least one major gene resistance to M. incognita located in this subtelomeric position. We showed that plum and peach genes are independent and, thus, can be pyramided into interspecific hybrid rootstocks based on the plum and peach species.

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“…The Ma resistance has a wide spectrum (Esmenjaud et al, 1994;Lecouls et al, 1997) and is not overcome under high inoculum pressure (Esmenjaud et al, 1996a), which suggests that this resistance response cannot be easily counteracted by the nematodes. The similarity of the PL domains to the WRKY transcription factors suggests that the key targets of Meloidogyne species could be WRKY transcription factors that are known to be essential for the plant defense response and development (Rushton et al, 2010) and, more specifically, for basal response to RKN in tomato and Arabidopsis (Bhattarai et al, 2010).…”

Section: Ma and Resistance To Rknmentioning

confidence: 99%

“…In Prunus species, among the RKN R genes mapped , the Ma gene from the Myrobalan plum (Prunus cerasifera) confers a complete-spectrum dominant resistance with several R alleles (Esmenjaud et al, 1996b;Lecouls et al, 1997) and is used directly or through interspecific hybrids for rootstock breeding in stone fruits using marker-assisted selection ). This gene is not overcome by any of the over 30 RKN species and isolates that have been tested (Esmenjaud et al, 1994).…”

mentioning

confidence: 99%

“…This gene is not overcome by any of the over 30 RKN species and isolates that have been tested (Esmenjaud et al, 1994). In particular, it also controls "the peach RKN," Meloidogyne floridensis (Handoo et al, 2004), which overcomes the resistance of all commonly used peach (Prunus persica) and almond (Prunus dulcis) R sources (Lecouls et al, 1997), and the highly aggressive species Meloidogyne mayaguensis (= Meloidogyne enterolobii; Yang and Eisenback, 1983), a species uncontrolled by the Mi-1 gene that is progressively invading new territories Cetintas et al, 2007;Nyczepir et al, 2008).…”

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confidence: 99%

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TheMaGene for Complete-Spectrum Resistance toMeloidogyneSpecies inPrunusIs a TNL with a Huge Repeated C-Terminal Post-LRR Region

et al. 2011

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Root-knot nematode (RKN) Meloidogyne species are major polyphagous pests of most crops worldwide, and cultivars with durable resistance are urgently needed because of nematicide bans. The Ma gene from the Myrobalan plum (Prunus cerasifera) confers complete-spectrum, heat-stable, and high-level resistance to RKN, which is remarkable in comparison with the Mi-1 gene from tomato (Solanum lycopersicum), the sole RKN resistance gene cloned. We report here the positional cloning and the functional validation of the Ma locus present at the heterozygous state in the P.2175 accession. High-resolution mapping totaling over 3,000 segregants reduced the Ma locus interval to a 32-kb cluster of three Toll/Interleukin1 Receptor-Nucleotide Binding Site-Leucine-Rich Repeat (LRR) genes (TNL1-TNL3), including a pseudogene (TNL2) and a truncated gene (TNL3). The sole complete gene in this interval (TNL1) was validated as Ma, as it conferred the same complete-spectrum and high-level resistance (as in P.2175) using its genomic sequence and native promoter region in Agrobacterium rhizogenes-transformed hairy roots and composite plants. The full-length cDNA (2,048 amino acids) of Ma is the longest of all Resistance genes cloned to date. Its TNL structure is completed by a huge post-LRR (PL) sequence (1,088 amino acids) comprising five repeated carboxylterminal PL exons with two conserved motifs. The amino-terminal region (213 amino acids) of the LRR exon is conserved between alleles and contrasts with the high interallelic polymorphisms of its distal region (111 amino acids) and of PL domains. The Ma gene highlights the importance of these uncharacterized PL domains, which may be involved in pathogen recognition through the decoy hypothesis or in nuclear signaling.

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Spectrum of the Ma genes for resistance to Meloidogyne spp. in Myrobalan plum

Cited by 45 publications

References 19 publications

Molecular Genetic Markers for Root-Knot Nematode Resistance in Some Peach Rootstocks

Molecular Genetic Markers for Root-Knot Nematode Resistance in Some Peach Rootstocks

Location of independent root-knot nematode resistance genes in plum and peach

TheMaGene for Complete-Spectrum Resistance toMeloidogyneSpecies inPrunusIs a TNL with a Huge Repeated C-Terminal Post-LRR Region

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