volume 32, issue 44, P11838-11846 1993
DOI: 10.1021/bi00095a012
View full text
Mahendra Kumar Jain, Badri P. Maliwal

Abstract: The near-UV absorption and fluorescence spectroscopic properties of Trp-3 of pig pancreatic phospholipase A2 (PLA2) in aqueous solution (E form) or at the interface without (E* form) or with a ligand at the active site (E*L form) are characterized. In the E form, the single tryptophan residue is exposed on the protein surface to the aqueous environment, as it is freely accessible to aqueous quenchers such as succinimide and acrylamide. The fluorescence quantum yield of E is about one-third that of N-acetyl-try…

expand abstract