Spectrofluorimetric Determination of Terbinafine Hydrochloride and Linezolid in their Dosage Forms and Human Plasma

Abstract

A highly sensitive, simple and rapid spectrofluorimetric method was developed for the determination of Terbinafine HCl (TRH) and linezolid (LNZ) in their pharmaceutical formulations. The proposed method is based on measuring the native fluorescence of the studied drugs in water at 336 nm after excitation at 275 nm for TRH and 375 nm after excitation at 254 nm for LNZ. The fluorescence-concentration plots were rectilinear over the range of 0.02-0.15 μg/mL for TRH and 0.5-5.0 μg/mL for LNZ. With lower detection limits of 3.0 and 110.0 ng/mL and a lower quantification limit of 9.0 and 320.0 ng/mL for TRH and LNZ, respectively. The method was successfully applied to the analysis of TRH in its commercial tablets, cream, gel and spray formulations and the results were in good agreement with those obtained with the official method. In addition the method was also applied to the analysis of LNZ in its capsule and I.V solution and the results were in good agreement with those obtained with the comparison method. The effect of sensitizers was studied. The method was extended to the determination of the studied drugs in spiked human plasma and the results were satisfactory.

Analytical methods for determination of terbinafine hydrochloride in pharmaceuticals and biological materials

“…Based on the measurement of native fluorescence of TFH in water at 376 nm after excitation at 275 nm, a rapid and highly sensitive spectrofluorimetric method has been reported for TFH in pharmaceuticals [22] . The fluorescence-concentration plot was linear over the concentration range of 0.02–0.05 µg/mL ( r =0.9998) with LOD and LOQ values of 0.0031 and 0.0094 µg/mL, respectively.…”

An effective and sensitive stability-indicating chromatographic approach based on HPLC for silodosin assay

“…Chromatographic and spectrofluorimetric approaches have been useful techniques for the determination of drug or biologically important molecules in real samples for many years, and these techniques offer a simple way to quantify the drug molecules especially in pharmaceutical formulations (Belal et al 2013;Antunes et al 2013). Therefore, the presented study aims to develop validated analytical methods for the determination of SIL in pharmaceutical samples.…”

Spectrofluorimetric determination of acotiamide hydrochloride trihydrate in the presence of its oxidative degradation product

“…Spectrofluorimetry, owing to its highly sensitive, selective, and inexpensive technique, has been widely applied in the last decade for the analysis of several pharmaceutical compounds …”