Spectral characteristics of fluorophores formed via interaction between all-trans-retinal with rhodopsin and lipids in photoreceptor membrane of retina rod outer segments

Loguinova, M. Yu.; Zagidullin, V. E.; Feldman, T. B.; Rostovtseva, Y. V.; Paschenko, V.Z.; Rubin, A. B.; Оstrovsky, М. А.

doi:10.1134/s1990747809020056

Cited by 7 publications

(8 citation statements)

References 23 publications

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“…Two findings associated with short fluorescence lifetimes merit further discussion. In the first instance, data from other studies 19 and from our previous studies 7 suggest that components of the visual cycle such as all-trans retinal (atRAL) display very short lifetimes. Under conditions of visual cycle dysfunction, retinoid cycle by-products have been shown to accumulate in outer segments of photoreceptor cells and the RPE.…”

Section: Discussionmentioning

confidence: 83%

Autofluorescence Lifetimes in Patients With Choroideremia Identify Photoreceptors in Areas With Retinal Pigment Epithelium Atrophy

Dysli

Wolf

Tran

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Section: Discussionmentioning

confidence: 83%

Autofluorescence Lifetimes in Patients With Choroideremia Identify Photoreceptors in Areas With Retinal Pigment Epithelium Atrophy

Dysli

Wolf

Tran

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…Using picosecond fluorometry, the lifetime (T1) of atRAL was shown to be 31 ps. 32 Our own measurements of pure retinal, using an excitation wavelength of 473 nm, revealed mean fluorescence decay times Tm of 38 ps in the emission channel between 560 and 720 nm. However, retinal is only an intermediate product and is rapidly reduced to atRAL by the retinol dehydrogenase and further transformed to secondary products such as A2E.…”

Section: Discussionmentioning

confidence: 85%

“…It has been shown that atRAL accumulates in photoreceptor outer segments in patients with STGD. 32 STGD is caused by dysfunction of ABCR, which transports atRAL from the inside to the outside of disc membranes. This leads to an accumulation of atRAL condensation products and subsequently to degeneration of RPE and photoreceptors.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence Lifetime Imaging in Stargardt Disease: Potential Marker for Disease Progression

Dysli

Wolf

Hatz

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Patients with recent disease onset showed flecks with very short autofluorescence lifetimes, which is possible evidence of accumulation of retinoids deriving from the visual cycle. During the study period, many of these flecks changed to longer lifetimes, possibly due to accumulation of lipofuscin. Therefore, FLIO might serve as a useful tool for monitoring of disease progression. (ClinicalTrials.gov number, NCT01981148.).

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“…While human FAF is attributed to all-trans-retinal (ATR)-derived compounds such as A2E and its precursor and oxidation products, ATR itself, which is highly concentrated in the retina, is basically non-fluorescent [39]. In this study the authors describe the accumulation of ATR derivatives formed in the process of interaction with amino groups of rhodopsin and with lipids by the incubation of rod outer segments membranes with a 10-fold excess of ATR over three days.…”

Section: Fluorescence and Possible Fluorophores From Within The Neuramentioning

confidence: 99%

Fundus autofluorescence beyond lipofuscin: lesson learned from ex vivo fluorescence lifetime imaging in porcine eyes

Hammer¹,

Sauer²,

Klemm³

et al. 2018

Biomed. Opt. Express

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Fundus autofluorescence (FAF) imaging is a well-established method in ophthalmology; however, the fluorophores involved need more clarification. The FAF lifetimes of 20 post mortem porcine eyes were measured in two spectral channels using fluorescence lifetime imaging ophthalmoscopy (FLIO) and compared with clinical data from 44 healthy young subjects. The FAF intensity ratio of the short and the long wavelength emission (spectral ratio) was determined. porcine fundus fluorescence emission is generally less intense than that seen in human eyes. The porcine retina showed significantly (p<0.05) longer lifetimes than the retinal pigment epithelium (RPE): 584 ± 128 ps vs. 121 ± 55 ps 498-560 nm, 240 ± 42 ps vs. 125 ± 20 ps at 560-720 nm. Furthermore, the lifetimes of the porcine RPE were significantly shorter (121 ± 55 ps and 125 ± 20 ps) than those measured from human fundus (162 ± 14 ps and 179 ± 13 ps, respectively). The fluorescence emission of porcine retina was shifted towards a shorter wavelength compared to that of RPE and human FAF. This data shows the considerable contribution of fluorophores in the neural retina to total FAF intensity in porcine eyes.

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Spectral characteristics of fluorophores formed via interaction between all-trans-retinal with rhodopsin and lipids in photoreceptor membrane of retina rod outer segments

Cited by 7 publications

References 23 publications

Autofluorescence Lifetimes in Patients With Choroideremia Identify Photoreceptors in Areas With Retinal Pigment Epithelium Atrophy

Autofluorescence Lifetimes in Patients With Choroideremia Identify Photoreceptors in Areas With Retinal Pigment Epithelium Atrophy

Fluorescence Lifetime Imaging in Stargardt Disease: Potential Marker for Disease Progression

Fundus autofluorescence beyond lipofuscin: lesson learned from ex vivo fluorescence lifetime imaging in porcine eyes

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