Specificity of synapse formation between <i>Lymnaea</i> heart motor neuron and muscle fiber is maintained in vitro in a soma–muscle configuration

Lee, Thomas K. M.; Leung, Alexander A. C.; Brezden, Boris L.; Lukowiak, Ken; Syed, Naweed I.

doi:10.1002/syn.10125

Cited by 3 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By extending these data, some specific synaptic-like proteins of vesicles, synapsin, and synaptophysin that had been detected in neuromuscular junctions of mammalian cultured cells (DeCamilli et al 1988) have been found in the neuronal cells of snails Aplysia punctata and Helix pornatia (Chin et al 1990;Bongiovi et al 1992;Cibelli et al 1996). Moreover, synapse formation between the heart motor neuron and muscle fiber of the snail Lymnaea stagnalis has been reconstructed in vitro (Lee et al 2002).…”

Section: Introductionmentioning

confidence: 95%

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

2010

View full text Add to dashboard Cite

Molluscan in vitro technology allows the study of the differentiation of isolated cells undergoing experimental manipulations. We have used the immunofluorescence technique and laser scanning microscopy to investigate the organization of muscle proteins (actin, myosin, paramyosin, and twitchin) and the localization of neurotransmitters (serotonin and FMRFamide) in cultured mussel larval cells. Differentiation into muscle and neuron-like cells occurs during the cultivation of mussel cells from premyogenic and prenervous larval stages. Muscle proteins are colocalized in contractile cells through all stages of cultivation. The cultivation of mussel cells on various substrates and the application of integrin receptor blockers suggest that an integrin-dependent mechanism is involved in cell adhesion and differentiation. Dissociated mussel cells aggregate and become self-organized in culture. After 20 days of cultivation, they form colonies in which serotonin- and FMRFamide-immunoreactive cells are located centrally, whereas muscle cells form a contractile network at the periphery. The pattern of thick and thin filaments in cultivated mussel cells changes according to the scenario of muscle arrangement in vivo: initially, a striated pattern of muscle filaments forms but is then replaced by a smooth muscle pattern with a diffuse distribution of muscle proteins, typical of muscles of adult molluscs. Myogenesis in molluscs thus seems to be a highly dynamic and potentially variable process. Such a "flexible" developmental program can be regarded as a prerequisite for the evolution of the wide variety of striated and smooth muscles in larval and adult molluscs.

show abstract

Section: Introductionmentioning

confidence: 95%

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

2010

View full text Add to dashboard Cite

show abstract

“…The dissociated cells were subsequently washed three times with defined medium and sedimented at low speed, before plating. Dissociated muscle fibers were kept in defined medium in Costar (Cambridge, MA) dishes at 20°C for 1-4 d (Lee et al, 2002). All neuronal culture procedures were performed as described previously (Syed et al, 1990).…”

Section: Cardiac Muscle Dissociation and Neuron Isolationmentioning

confidence: 99%

Peptidomics of a Single Identified Neuron Reveals Diversity of Multiple Neuropeptides with Convergent Actions on Cellular Excitability

Jiménez

Spijker

Schipper³

et al. 2006

J. Neurosci.

View full text Add to dashboard Cite

In contrast to classical transmitters, the detailed structures and cellular and synaptic actions of neuropeptides are less well described. Peptide mass profiling of single identified neurons of the mollusc Lymnaea stagnalis indicated the presence of 17 abundant neuropeptides in the cardiorespiratory neuron, visceral dorsal 1 (VD1), and a subset of 14 peptides in its electrically coupled counterpart, right parietal dorsal 2. Altogether, based on this and previous work, we showed that the high number of peptides arises from the expression and processing of four distinct peptide precursor proteins, including a novel one. Second, we established a variety of posttranslational modifications of the generated peptides, including phosphorylation, disulphide linkage, glycosylation, hydroxylation, N-terminal pyroglutamylation, and C-terminal amidation. Specific synapses between VD1 and its muscle targets were formed, and their synaptic physiology was investigated. Whole-cell voltage-clamp analysis of dissociated heart muscle cells revealed, as tested for a selection of representative family members and their modifications, that the peptides of VD1 exhibit convergent activation of a high-voltage-activated Ca current. Moreover, the differentially glycosylated and hydroxylated ␣2 peptides were more potent than the unmodified ␣2 peptide in enhancing these currents. Together, this study is the first to demonstrate that single neurons exhibit such a complex pattern of peptide gene expression, precursor processing, and differential peptide modifications along with a remarkable degree of convergence of neuromodulatory actions. This study thus underscores the importance of a detailed mass spectrometric analysis of neuronal peptide content and peptide modifications related to neuromodulatory function.

show abstract

“…The synaptic inputs of motoneurons into the heart are conserved even in vitro , i.e. if the muscle fibers and these neurons are kept in culture in close proximity, they will develop functional connections (Lee et al, 2002). Interestingly, even neurites can survive and function without soma in vitro .…”

Section: Excitation Of Heartmentioning

confidence: 99%

The neuronal control of cardiac functions in Molluscs

Kodirov

2011

Comparative Biochemistry and Physiology Part A: Molecular &

View full text Add to dashboard Cite

In this manuscript, I review the current and relevant classical studies on properties of the Mollusca heart and their central nervous system including ganglia, neurons, and nerves involved in cardiomodulation. Similar to mammalian brain hemispheres, these invertebrates possess symmetrical pairs of ganglia albeit visceral (only one) ganglion and the parietal ganglia (the right ganglion is bigger than the left one). Furthermore, there are two major regulatory drives into the compartments (pericard, auricle, and ventricle) and cardiomyocytes of the heart. These are the excitatory and inhibitory signals that originate from a few designated neurons and their putative neurotransmitters. Many of these neurons are well-identified, their specific locations within the corresponding ganglion are mapped, and some are termed as either heart excitatory (HE) or inhibitory (HI) cells. The remaining neurons are classified as cardio-regulatory, and their direct and indirect actions on the heart’s function have been documented. The cardiovascular anatomy of frequently used experimental animals, Achatina, Aplysia, Helix, and Lymnaea is relatively simple. However, as in humans, it possesses all major components including even trabeculae and atrio-ventricular valves. Since the myocardial cells are enzymatically dispersible, multiple voltage dependent cationic currents in isolated cardiomyocytes are described. The latter include at least the A-type K+, delayed rectifier K+, TTX-sensitive Na+, and L-type Ca2+ channels.

show abstract

Specificity of synapse formation between Lymnaea heart motor neuron and muscle fiber is maintained in vitro in a soma–muscle configuration

Cited by 3 publications

References 25 publications

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

Peptidomics of a Single Identified Neuron Reveals Diversity of Multiple Neuropeptides with Convergent Actions on Cellular Excitability

The neuronal control of cardiac functions in Molluscs

Contact Info

Product

Resources

About