Specificity and preference of mycorrhizal associations in two species of the genus Dendrobium (Orchidaceae)

Xing, Xiaoke; Ma, Xiu-Wen; Deng, Zhenhai; Chen, Juan; Wu, Fengzhi; Guo, Shun‐Xing

doi:10.1007/s00572-012-0473-8

Cited by 37 publications

(25 citation statements)

References 41 publications

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“…EBLFs), may have affected the diversification of Orchidaceae (including Dendrobium ) (Darwin, ; van der Cingel, ; Cozzolino & Widmer, ). Although few studies have focused on the roles of mycorrhizal fungi and the pollinators of this genus, recent results have indicated a low specificity of mycorrhizal fungi in some species of Dendrobium and in epiphytic orchids more generally (Xing et al ., ). Moreover, while several pollination mechanisms have been illustrated in Dendrobium , including autogamy, deceptive pollination and possible sleeping site rewards (Brodmann et al ., ; Kowalkowska & Margonska, ; Pang et al ., ), it seems that most Dendrobium species rely on deceptive pollination (van der Cingel, ; Jersáková et al ., ), which indicates that EBLFs provide rewards for deceived pollinators.…”

Section: Discussionmentioning

confidence: 99%

Biogeographical diversification of mainland Asian Dendrobium (Orchidaceae) and its implications for the historical dynamics of evergreen broad‐leaved forests

Xiao

Zhou

et al. 2016

Journal of Biogeography

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Aim Evergreen broad-leaved forests (EBLFs) occupy most tropical and subtropical regions of mainland Asia and exhibit high species diversity, productivity and richness. Understanding the historical dynamics of EBLFs is important for biodiversity conservation and regional carbon storage and cycling under global climate change. However, little is known about the historical dynamics of EBLFs in mainland Asia. Dendrobium, an epiphytic orchid genus found in EBLFs, was used to gain new insights of the historical establishment and extension of EBLFs in mainland Asia. Location Mainland Asia.Methods We sampled c. 80% of the currently recognized species of Dendrobium found in mainland Asia. A phylogeny was generated using maximum parsimony, maximum likelihood and Bayesian inference methods. A calibrated chronogram was obtained using a Bayesian relaxed-clock model approach. Biogeographical scenarios were investigated using the statistical dispersal-vicariance analysis and dispersal-extinction-cladogenesis methods. Ancestral states were constructed by mesquite and bayestraits, and diversification of Dendrobium was investigated by APE and LASER packages.Results Our results indicate that Asian Dendrobium was present on mainland Asia since the Oligocene, after which this genus diversified in this region and dispersed into higher elevations. The ancestral habit of Dendrobium was epiphytic and terrestrial and lithophytic habits each have multiple, recently independent origins.Main conclusions Our results support the hypothesis that EBLFs have been established in mainland Asia at least since the Oligocene and further suggest that the EBLFs of mainland Asia expanded into higher elevations prior to the late Cenozoic. The diversification of Asian Dendrobium coincided with a warmer climate during the late Oligocene and middle Miocene and the expansion of key groups (including Fagaceae, Lauraceae, Magnoliaceae and Theaceae) of EBLFs during the early Cenozoic.

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Section: Discussionmentioning

confidence: 99%

Biogeographical diversification of mainland Asian Dendrobium (Orchidaceae) and its implications for the historical dynamics of evergreen broad‐leaved forests

Xiao

Zhou

et al. 2016

Journal of Biogeography

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“…; Xing et al . ), implying that to accurately describe mycorrhizal associations in orchids fungal communities should be assessed, rather than the presence of individual fungal species or strains.…”

Section: Introductionmentioning

confidence: 99%

Impact of primer choice on characterization of orchid mycorrhizal communities using 454 pyrosequencing

Waud

Busschaert

Ruyters

et al. 2014

Molecular Ecology Resources

108

109

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Although the number of studies investigating mycorrhizal associations in orchids has increased in recent years, the fungal communities associating with orchids and how they differ between species and sites remain unclear. Recent research has indicated that individual orchid plants may associate with several fungi concurrently, implying that to study mycorrhizal associations in orchids the fungal community should be assessed, rather than the presence of individual dominant fungal species or strains. High-throughput sequencing methods, such as 454 pyrosequencing, are increasingly used as the primary tool for such analyses. However, many studies combine universal primers from previous phylogenetic or ecological studies to generate amplicons suitable for 454 pyrosequencing without first critically evaluating their performance, potentially resulting in biased fungal community descriptions. Here, following in silico primer analysis we evaluated the performance of different combinations of existing PCR primers to characterize orchid mycorrhizal communities using 454 pyrosequencing by analysis of both an artificially assembled community of mycorrhizal fungi isolated from diverse orchid species and root samples from three different orchid species (Anacamptis morio, Ophrys tenthredinifera and Serapias lingua). Our results indicate that primer pairs ITS3/ITS4OF and ITS86F/ITS4, targeting the internal transcribed spacer-2 (ITS-2) region, outperformed other tested primer pairs in terms of number of reads, number of operational taxonomic units recovered from the artificial community and number of different orchid mycorrhizal associating families detected in the orchid samples. Additionally, we show the complementary specificity of both primer pairs, making them highly suitable for tandem use when studying the diversity of orchid mycorrhizal communities.

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“…Concurrent association of several fungi with an individual orchid is common, especially in photosynthetic orchids [ 17 - 20 ], suggesting the importance of studying the whole community of mycorrhizal fungi rather than the presence of individual fungal species. However, current mycorrhizal fungi identification requires microscopic identification and culturing or DNA segment cloning/sequencing, which may not provide sufficient resolution for the whole fungal community in the plant [ 14 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Highly diversified fungi are associated with the achlorophyllous orchid Gastrodia flavilabella

Liu

Han

et al. 2015

BMC Genomics

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BackgroundMycoheterotrophic orchids are achlorophyllous plants that obtain carbon and nutrients from their mycorrhizal fungi. They often show strong preferential association with certain fungi and may obtain nutrients from surrounding photosynthetic plants through ectomycorrhizal fungi. Gastrodia is a large genus of mycoheterotrophic orchids in Asia, but Gastrodia species’ association with fungi has not been well studied. We asked two questions: (1) whether certain fungi were preferentially associated with G. flavilabella, which is an orchid in Taiwan and (2) whether fungal associations of G. flavilabella were affected by the composition of fungi in the environment.ResultsUsing next-generation sequencing, we studied the fungal communities in the tubers of Gastrodia flavilabella and the surrounding soil. We found (1) highly diversified fungi in the G. flavilabella tubers, (2) that Mycena species were the predominant fungi in the tubers but minor in the surrounding soil, and (3) the fungal communities in the G. flavilabella tubers were clearly distinct from those in the surrounding soil. We also found that the fungal composition in soil can change quickly with distance.ConclusionsG. flavilabella was associated with many more fungi than previously thought. Among the fungi in the tuber of G. flavilabella, Mycena species were predominant, different from the previous finding that adult G. elata depends on Armillaria species for nutritional supply. Moreover, the preferential fungus association of G. flavilabella was not significantly influenced by the composition of fungi in the environment.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1422-7) contains supplementary material, which is available to authorized users.

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Specificity and preference of mycorrhizal associations in two species of the genus Dendrobium (Orchidaceae)

Cited by 37 publications

References 41 publications

Biogeographical diversification of mainland Asian Dendrobium (Orchidaceae) and its implications for the historical dynamics of evergreen broad‐leaved forests

Biogeographical diversification of mainland Asian Dendrobium (Orchidaceae) and its implications for the historical dynamics of evergreen broad‐leaved forests

Impact of primer choice on characterization of orchid mycorrhizal communities using 454 pyrosequencing

Highly diversified fungi are associated with the achlorophyllous orchid Gastrodia flavilabella

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