Specification ofDrosophilaaCC motoneuron identity by a genetic cascade involvingeven-skipped, grainandzfh1

Garcès, Alain; Thor, Stefan

doi:10.1242/dev.02321

Cited by 44 publications

(41 citation statements)

References 58 publications

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“…Our data support the previous findings that in the absence of GATA3, VCEs are reduced in their projection across the midline and fail to target the ear, thus adding to the growing body of evidence that GATA3 facilitates neuronal guidance across phyla. For example, another Drosophila GATA3 ortholog Grn appears to be necessary for the specification of a subset of neurons in the fly as well as necessary for their correct guidance (Garces and Thor, 2006). However, we show here that these neurons are not absent or fail to migrate their soma to the correct location in the hindbrain, indicating that GATA3 is not essential for these aspects of neuronal development of VCEs.…”

Section: Discussioncontrasting

confidence: 61%

Limited inner ear morphogenesis and neurosensory development are possible in the absence of GATA3

Duncan

Lim

Engel³

et al. 2011

Int. J. Dev. Biol.

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Haploinsufficiency of Gata3 causes hypoparathyroidism, deafness and renal dysplasia (HDR) syndrome in mice and humans. Gata3 null mutation leads to early lethality around embryonic day (E)11.5, but catecholamine precursor administration can rescue Gata3 null mutants to E16.5. At E11.5, GATA3 deficiency results in the development of an empty otocyst with an endolymphatic duct. However, using rescued mice we found that some morphogenesis and neurosensory development is possible in the ear without Gata3. Extending previous studies, we find that at E16.5, Gata3 mutant inner ears can undergo partial morphogenesis and develop an endolymphatic duct, a utricular and saccular recess, and a shortened cochlear duct. In addition to the obvious morphogenic aberrations, these studies demonstrate that a subset of neurons develop and connect a fragmented sensory patch of MYO7A-positive hair cells to the vestibular nuclei of the brainstem. In situ hybridization studies reveal altered expression of several transcription factors relevant to ear development and we hypothesize that this may relate to the observed dysmorphia and restricted neurosensory development. While a cochlear duct can form, there is no concurrent cochlear neurosensory development, observations consistent with specific hearing defects encountered by HDR patients and mice with Gata3-associated expression alterations. Gata3 null mutant phenocopies the otic maldevelopment (cochlear duct formation in the absence of neurosensory development) seen in Foxg1cre mediated conditional deletion of microRNA processing enzyme, Dicer1. Finally, while GATA3 is expressed in the developing vestibulocochlear efferent (VCE) neurons, and its absence in the null mutants disrupts VCE projections to the ear, loss of GATA3 does not affect VCE progenitor cell migration.

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Section: Discussioncontrasting

confidence: 61%

Limited inner ear morphogenesis and neurosensory development are possible in the absence of GATA3

Duncan

Lim

Engel³

et al. 2011

Int. J. Dev. Biol.

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“…The following Drosophila lines were used: btl-Gal4 (Shiga et al, 2014), nullo-Gal4 (Kunwar et al, 2003), 69B-Gal4 (Brand and Perrimon, 1993), sal-TSE-lacZ (Kühnlein and Schuh, 1996), grn-Gal4 (Garces and Thor, 2006), UAS-ct (Ludlow et al, 1996) and UAS-sal (Kühnlein and Schuh, 1996). Crosses with GAL4 lines were reared at either 25°C or 29°C.…”

Section: Insect Stocksmentioning

confidence: 99%

Genetic basis for the evolution of organ morphogenesis: the case of spalt and cut in the development of insect trachea

et al. 2016

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It is not clear how simple genetic changes can account for the coordinated variations that give rise to modified functional organs. Here, we addressed this issue by analysing the expression and function of regulatory genes in the developing tracheal systems of two insect species. The larval tracheal system of Drosophila can be distinguished from the less derived tracheal system of the beetle Tribolium by two main features. First, Tribolium has lateral spiracles connecting the trachea to the exterior in each segment, while Drosophila has only one pair of posterior spiracles. Second, Drosophila, but not Tribolium, has two prominent longitudinal branches that distribute air from the posterior spiracles. Both innovations, while considered different structures, are functionally dependent on each other and linked to habitat occupancy. We show that changes in the domains of spalt and cut expression in the embryo are associated with the acquisition of each structure. Moreover, we show that these two genetic modifications are connected both functionally and genetically, thus providing an evolutionary scenario by which a genetic event contributes to the joint evolution of functionally inter-related structures.

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“…The genes nab and klumpfuss are required to specify ABLK neuronal fate There is increasing evidence that neuronal fate specification is a multistep process involving combinatorial gene expression codes that specify neuronal properties (Allan et al, 2005;Baumgardt et al, 2007;Certel and Thor, 2004;Garces and Thor, 2006). In order to identify genes involved in specification of the ABLK fate, we analyzed the expression of Lk in embryos mutant for genes known to be required for CNS development.…”

Section: Fig 5 Requirement For Notch Signaling In Ablk Specificatiomentioning

confidence: 99%

A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS

et al. 2010

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SUMMARYIdentification of the genetic mechanisms underlying the specification of large numbers of different neuronal cell fates from limited numbers of progenitor cells is at the forefront of developmental neurobiology. In Drosophila, the identities of the different neuronal progenitor cells, the neuroblasts, are specified by a combination of spatial cues. These cues are integrated with temporal competence transitions within each neuroblast to give rise to a specific repertoire of cell types within each lineage. However, the nature of this integration is poorly understood. To begin addressing this issue, we analyze the specification of a small set of peptidergic cells: the abdominal leucokinergic neurons. We identify the progenitors of these neurons, the temporal window in which they are specified and the influence of the Notch signaling pathway on their specification. We also show that the products of the genes klumpfuss, nab and castor play important roles in their specification via a genetic cascade.

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Specification ofDrosophilaaCC motoneuron identity by a genetic cascade involvingeven-skipped, grainandzfh1

Cited by 44 publications

References 58 publications

Limited inner ear morphogenesis and neurosensory development are possible in the absence of GATA3

Limited inner ear morphogenesis and neurosensory development are possible in the absence of GATA3

Genetic basis for the evolution of organ morphogenesis: the case of spalt and cut in the development of insect trachea

A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS

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