DOI: 10.17265/2159-5828/2012.09.037
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Xihong Zhao, Jun Wang, Joong-Hyun Park, Fereidoun Forghani, Myoungsu Parkand Deog-Hwan Oh

Abstract: Traditional molecular detection methods cannot distinguish Escherichia coli O157:H7 in viable or dead state. In this study, the loop-mediated is other mal amplification (LAMP) method combined with propidium monoazide (PMA) treatment was developed to selectively detect the viable Escherichia coli O157:H7, but not dead cells. Four primers, including outer primers and inner primers, were specially designed for recognizing six distinct sequences on the species-specific rfbE gene of Escherichia coli O157:H7 genome.…

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