Specific detection and effective inhibition of a single bacterial species in situ using peptide mineralized Au cluster probes

Zhang, Xiangchun; Liu, Li; Liu, Ru; Wang, Jing; Hu, Xuhu; Yuan, Qing; Guo, Juanjuan; Xing, Gengmei; Zhao, Yuliang; Gao, Xueyun

doi:10.1007/s11426-017-9206-y

Cited by 13 publications

(8 citation statements)

References 49 publications

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“…Hence, there is an urgent need to invent new antimicrobial agents. Recent, research efforts are mainly concerned with the modification of current antibiotics, searching for new antibiotics, antimicrobial agents − and developing antimicrobial polypeptide (AMP). − In contrast to antibiotics, host-defended AMPs (a vital component of the innate immune system) interact with bacteria by a nonspecific (electrostatic) mechanism which enables them to combat the MDR bacteria efficiently . The cationic moiety of an AMP interacts with the negatively charged phosphate head of lipopolysaccharides (LPSs) which persists on the bacterial cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Coordination-Assisted Self-Assembled Polypeptide Nanogels to Selectively Combat Bacterial Infection

Panja

Bharti

Dey

et al. 2019

ACS Appl. Mater. Interfaces

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In the present scenario, the invention of bacteria-selective antimicrobial agent comprising negligible toxicity and hemolytic effect is a great challenge. To surmount this challenge, here, a series of polypeptide nanogels (PNGs) have been fabricated by a coordination-assisted self-assembly of a mannose-conjugated antimicrobial polypeptide, poly(arginine-r-valine)-mannose (poly(Arg-r-Val)-M2), with Zn2+ ions. The fabricated PNGs are spherical in shape with a unique structural appearance similar to that of Taxus baccata fruits. PNGs, with a unique structural arrangement and threshold surface charge density, selectively interact with the bacterial membrane and exhibit potent antimicrobial activity, as reflected in their lower minimum inhibitory concentration values (varies from 2 to 16 μg/mL). PNGs show a remarkably high binding constant, 6.02 × 105 M–1 (from isothermal titration calorimetry, ITC), with the bacterial membrane which manifests its potent bactericidal effect. PNGs are nontoxic against mammalian and red blood cells as reflected from their higher cell viability and insignificant hemolytic effect. PNGs are taken up by the bacterial membrane and selectively undergo structural deformation (scrutinized by ITC) followed by an exposure of free poly(Arg-r-Val)-M2 molecules. The free poly(Arg-r-Val)-M2 molecules are enforced to lyse the bacterial membrane (visualized by cryo-transmission electron microscopy) followed by the diffusion of the cytoplasmic component out of the membrane which culminates in the final death of the bacterium.

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Section: Introductionmentioning

confidence: 99%

Coordination-Assisted Self-Assembled Polypeptide Nanogels to Selectively Combat Bacterial Infection

Panja

Bharti

Dey

et al. 2019

ACS Appl. Mater. Interfaces

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“…When the production of excessive ROS exceeds the scavenging ability of MRSA itself, it will cause fatal damage to the bacteria. Excessive ROS will attack important macromolecules in bacterial cell (e.g., nucleic acid, proteins and lipid), and eventually cause cell death 43,44 . As depicted in Figure 5E,F, the level of ROS in MRSA was significantly increased after PCLA/KH‐HA‐EGCG treatment, while no obvious ROS signal was observed in MRSA treatment with PCLA/KH‐HA without EGCG modified.…”

Section: Resultsmentioning

confidence: 94%

“…42 As depicted in Excessive ROS will attack important macromolecules in bacterial cell (e.g., nucleic acid, proteins and lipid), and eventually cause cell death. 43,44 As depicted in Figure 5E,F, the level of ROS in MRSA was significantly increased after PCLA/KH-HA-EGCG treatment, while no obvious ROS signal was observed in MRSA treatment with PCLA/KH-HA without EGCG modified. These results collectively demonstrated that the EGCG-modified scaffolds had good stability and biosafety, and could effectively induce endogenous ROS burst, destroy MRSA wall structure, and ultimately lead to bacterial death (Figures 4 and 5).…”

Section: Pcla/kh-ha-egcg Scaffold Inhibits Bacteria In Vitromentioning

confidence: 89%

3D printed PCLA scaffold with nano‐hydroxyapatite coating doped green tea EGCG promotes bone growth and inhibits multidrug‐resistant bacteria colonization

Zhang

Qiao

et al. 2022

Cell Proliferation

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Objectives: 3D-printing scaffold with specifically customized and biomimetic structures gained significant recent attention in tissue engineering for the regeneration of damaged bone tissues. However, constructed scaffolds that simultaneously promote bone regeneration and in situ inhibit bacterial proliferation remains a great challenge.This study aimed to design a bone repair scaffold with in situ antibacterial functions.Materials and Methods: Herein, a general strategy is developed by using epigallocatechin-3-gallate (EGCG), a major green tea polyphenol, firmly anchored in the nano-hydroxyapatite (HA) and coating the 3D printed polymerization of caprolactone and lactide (PCLA) scaffold. Then, we evaluated the stability, mechanical properties, water absorption, biocompatibility, and in vitro antibacterial and osteocyte inductive ability of the scaffolds. Results:The coated scaffold exhibit excellent activity in simultaneously stimulating osteogenic differentiation and in situ resisting methicillin-resistant Staphylococcus aureus colonization in a bone repair environment without antibiotics. Meanwhile, the prepared 3D scaffold has certain mechanical properties (39.3 ± 3.2 MPa), and the applied coating provides the scaffold with remarkable cell adhesion and osteogenic conductivity.Xiangchun Zhang and Jian He contributed equally to this study.

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“…Noble metal nanoclusters (NCs) consisting of several to hundreds of noble metal atoms and several ligands are usually employed for biomedical applications because of their special optical characteristic. , Because of these fluorescent properties as well as other desirable physical and chemical attributes, noble metal NCs are increasingly being applied in bioanalysis, biosensing, and as enzyme-mimicking catalysts. − Among the noble metal NCs (a group that includes Ag, Au, or Pt), Au NCs have received the most attention because of their high stability, ease in preparation, and good biocompatibility characteristics, which make them good candidates for biomedical and biosensor application. − Generally, Au NCs are prepared using proteins, , polypeptides, − or small organic molecules as templates. Such templates bind to the surface of the NCs to prevent aggregation and, in the case of proteins and peptides, can be easily modified for particular applications .…”

Section: Introductionmentioning

confidence: 99%

Polypeptide-Templated Au Nanoclusters with Red and Blue Fluorescence Emissions for Multimodal Imaging of Cell Nuclei

Liu

Zhang

Han

et al. 2020

ACS Appl. Bio Mater.

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Noble metal nanoclusters (<2 nm) prepared using special molecules such as proteins and peptides as templates have been attracting increasing attention in biomedical applications and cell imaging because of their excellent biocompatibility, size-dependent fluorescence properties, and relatively simple synthesis. Although cell imaging using metal clusters has developed rapidly in recent years, multimodal imaging of the cell nucleus remains challenging. Herein, we report the successful synthesis of two different types of gold nanoclusters, one with blue fluorescence emission (λex 320 nm, λem 405 nm) and the other with red fluorescence emission (λex 560 nm, λem 657 nm), using the same polypeptide SV (sequence: NH2–CCYGGPKKKRKVG-COOH) as a template. The synthesis of these two optically distinct Au nanoclusters (Au NCs) was achieved through the optimization of synthesis conditions, including light, pH, the Au: polypeptide ratio, reaction time, and temperature. The multimodal imaging ability of the Au NCs were subsequently explored, with particular emphasis on the enzyme-like catalysis of the NCs and their use in the optical imaging of cell nuclei. Results show that the Au NCs possessed similar biological activities as the parent peptide used in their synthesis as well as demonstrating peroxidase-like activity due to the Au nanocluster core. Further, the Au NCs targeted the cell nucleus, with their strong fluorescence allowing cell imaging (cells containing Au clusters could be distinguished by eye, eliminating the need for additional labels). Finally, the Au NCs exhibited excellent multimodal cell imaging abilities. The results suggest that the Au NCs introduced here are promising candidates for the labeling and analysis of pathological samples.

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Specific detection and effective inhibition of a single bacterial species in situ using peptide mineralized Au cluster probes

Cited by 13 publications

References 49 publications

Coordination-Assisted Self-Assembled Polypeptide Nanogels to Selectively Combat Bacterial Infection

Coordination-Assisted Self-Assembled Polypeptide Nanogels to Selectively Combat Bacterial Infection

3D printed PCLA scaffold with nano‐hydroxyapatite coating doped green tea EGCG promotes bone growth and inhibits multidrug‐resistant bacteria colonization

Polypeptide-Templated Au Nanoclusters with Red and Blue Fluorescence Emissions for Multimodal Imaging of Cell Nuclei

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