Specific and sensitive detection of nucleic acids and RNases using gold nanoparticle–RNA–fluorescent dye conjugates

Kim, Joong H.; Estabrook, R. August; Braun, Gary B.; Lee, Briana R.; Reich, Norbert O.

doi:10.1039/b710306a

Cited by 54 publications

(39 citation statements)

References 21 publications

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“…Gold nanoparticles have potential as nano-quenchers for DNA detection [26,[35][36][37][38][39]. The detection method is based on the distance dependence of the fluorescence of a fluorophore that is bound to gold nanoparticles through a DNA linker which is also used for DNA sensing.…”

Section: In Vitro Dna Detectionmentioning

confidence: 99%

Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications

Huang

El‐Sayed

2010

Metal‐Enhanced Fluorescence

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Gold nanoparticles have generated widespread interests for centuries. They are optically active in multiple modalities useful for biomédical imaging including fluorescence, absorption, scattering and Raman spectroscopy. In fluorescence, gold can serve as a fluorescent probe under certain conditions, or modify the signal of an adjacent fluorophore. The effect of gold nanoparticles on fluorescence spectroscopy depends on the stimulating light source, the relation of gold nanoparticle to an adjacent fluorohpore and the surrounding medium. For instance, gold particles can enhance or quench fluorescence of adjacent fluorophores or can quench fluorophores to which they are covalently bound.To better comprehend how gold nanoparticles can demonstrate multiple effects with a range of potential biomédical applications, an understanding of the particle-light interaction is necessary. When stimulated by the electromagnetic field of the light, the free electrons of the metal nanoparticle undergo a collective coherent oscillation on the surface of the ionic metallic lattice (Figure 20.1). Figure 20.1: Surface plasmon resonance of gold nanoparticles.This collective oscillations of the free electrons confined on the particle surface is called surface plasmon. The surface plasmon is resonant at a specific frequency of the incident light called surface plasmon resonance (SPR). This optical behavior of plasmonic nanoparticles can be explained by Mie theory [1] which involves the salvation of the maxwell's equation for an electromagnetic light wave interacting with a small sphere. For nanoparticles much smaller than the wavelength of light (< 20nm), only the dipole oscillation contributes significantly to the extinction cross section and thus Mie's theory is reduced to the following equation:

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Section: In Vitro Dna Detectionmentioning

confidence: 99%

Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications

Huang

El‐Sayed

2010

Metal‐Enhanced Fluorescence

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“…Very recently, a gold nanoparticle-RNA-fluorescent dye conjugate has been used for the detection of RNase H activity. [18] However, the need for functionalized thiol and dyemodified oligonucleotide probes added to the complexity, Abstract: We demonstrate a unique quadruplex-based fluorescence assay for sensitive, facile, real-time, and label-free detection of RNase H activity and inhibition by using a G-quadruplex formation strategy. In our approach, a RNA-DNA substrate was prepared, with the DNA strand designed as a quadruplex-forming oligomer.…”

Section: Introductionmentioning

confidence: 99%

A Quadruplex‐Based, Label‐Free, and Real‐Time Fluorescence Assay for RNase H Activity and Inhibition

Huang

et al. 2010

Chemistry A European J

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We demonstrate a unique quadruplex-based fluorescence assay for sensitive, facile, real-time, and label-free detection of RNase H activity and inhibition by using a G-quadruplex formation strategy. In our approach, a RNA-DNA substrate was prepared, with the DNA strand designed as a quadruplex-forming oligomer. Upon cleavage of the RNA strand by RNase H, the released G-rich DNA strand folds into a quadruplex in the presence of monovalent ions and interacts with a specific G-quadruplex binder, N-methyl mesoporphyrin IX (NMM); this gives a dramatic increase in fluorescence and serves as a reporter of the reaction. This novel assay is simple in design, fast in operation, and is more convenient and promising than other methods. It takes less than 30 min to finish and the detection limit is much better or at least comparable to previous reports. No sophisticated experimental techniques or chemical modification for either RNA or DNA are required. The assay can be accomplished by using a common spectrophotometer and obviates possible interference with the kinetic behavior of the catalysts. Our approach offers an ideal system for high-throughput screening of enzyme inhibitors and demonstrates that the structure of the G-quadruplex can be used as a functional tool in specific fields in the future.

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“…In the case of our catalytic reaction on the AuNPs, the catalytic fluorescence recovery was initiated by the co-incubation of the unpaired substrate on the AuNPs and lead DNAzymes with Pb 2+ and thus, the separated DNAzymes could form new pairs with the intact substrates that immobilized the AuNPs. The repeatable pairing of DNAzymes with the intact substrates on the AuNPs might accelerate the fluorescence recovery at a given incubation time (Kim et al, 2007). For an accurate judgment of the presence of Pb 2+ , a false positive signal with other metal ions should be excluded.…”

Section: Sensitivity and Specificity For Pb 2+ Detectionmentioning

confidence: 99%

Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles

Kim

Han

Chung

2011

Biosensors and Bioelectronics

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Specific and sensitive detection of nucleic acids and RNases using gold nanoparticle–RNA–fluorescent dye conjugates

Abstract: Gold nanoparticles were modified with RNA and utilized to detect specific DNA sequences and various RNA nucleases.

Cited by 54 publications

References 21 publications

Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications

Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications

A Quadruplex‐Based, Label‐Free, and Real‐Time Fluorescence Assay for RNase H Activity and Inhibition

Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles

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