Species specificity in the activation of Xer recombination at <i>dif</i> by FtsK

Yates, James R.; Aroyo, Mira; Sherratt, David J.; Barre, François‐Xavier

doi:10.1046/j.1365-2958.2003.03574.x

Cited by 52 publications

(71 citation statements)

References 39 publications

(53 reference statements)

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“…4). E. coli FtsK can activate XerCD Ng /dif Ng recombination, although inefficiently, suggesting that activation is partly species-specific, as previously reported for E. coli and Haemophilus influenzae (26). The dif GGI site differs from dif Ng at four positions all included in the XerD-binding site (Fig.…”

Section: Discussionsupporting

confidence: 70%

FtsK translocation permits discrimination between an endogenous and an imported Xer/ dif recombination complex

Fournes

Crozat

Pages

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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In bacteria, the FtsK/Xer/dif (chromosome dimer resolution site) system is essential for faithful vertical genetic transmission, ensuring the resolution of chromosome dimers during their segregation to daughter cells. This system is also targeted by mobile genetic elements that integrate into chromosomal dif sites. A central question is thus how Xer/dif recombination is tuned to both act in chromosome segregation and stably maintain mobile elements. To explore this question, we focused on pathogenic Neisseria species harboring a genomic island in their dif sites. We show that the FtsK DNA translocase acts differentially at the recombination sites flanking the genomic island. It stops at one Xer/dif complex, activating recombination, but it does not stop on the other site, thus dismantling it. FtsK translocation thus permits cis discrimination between an endogenous and an imported Xer/dif recombination complex.

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Section: Discussionsupporting

confidence: 70%

FtsK translocation permits discrimination between an endogenous and an imported Xer/ dif recombination complex

Fournes

Crozat

Pages

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…This is composed of two paralogous tyrosine recombinases (integrases), XerC and XerD, which cooperatively catalyze strand exchanges at a 28-bp DNA sequence, the dif site, which must be located at the junction of the two replichores to be functional [3,6–8]. Xer recombination is intimately coupled to cell division [9] through the septal protein FtsK [10–12], a DNA translocase [8] with an essential N-terminal transmembrane domain involved in its localization at the septum [13], and a C-terminal DNA motor domain involved in positioning and synapsing the two dif sites of the chromosome dimer at the division septum [12,14–19] as well as in activating the strand exchange [8] by direct interaction with XerD [20,21]. …”

Section: Introductionmentioning

confidence: 99%

“…influenzae obeys the E. coli paradigm [21], whereas in B. subtilis, the model bacteria for firmicutes (formerly known as low GC-content Gram-positive bacteria), neither of the two FtsK analogs (SpoIIIE and YtpT) appears able to drive Xer recombination [26]. Several attempts have been made to identify the Xer recombination machinery in Streptococci, a taxonomic group belonging to firmicutes and comprising major pathogens [29] as well as innocuous food-grade species of major industrial importance [30,31].…”

Section: Introductionmentioning

confidence: 99%

The Unconventional Xer Recombination Machinery of Streptococci/Lactococci

et al. 2007

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Homologous recombination between circular sister chromosomes during DNA replication in bacteria can generate chromosome dimers that must be resolved into monomers prior to cell division. In Escherichia coli, dimer resolution is achieved by site-specific recombination, Xer recombination, involving two paralogous tyrosine recombinases, XerC and XerD, and a 28-bp recombination site (dif) located at the junction of the two replication arms. Xer recombination is tightly controlled by the septal protein FtsK. XerCD recombinases and FtsK are found on most sequenced eubacterial genomes, suggesting that the Xer recombination system as described in E. coli is highly conserved among prokaryotes. We show here that Streptococci and Lactococci carry an alternative Xer recombination machinery, organized in a single recombination module. This corresponds to an atypical 31-bp recombination site (dif SL) associated with a dedicated tyrosine recombinase (XerS). In contrast to the E. coli Xer system, only a single recombinase is required to recombine dif SL, suggesting a different mechanism in the recombination process. Despite this important difference, XerS can only perform efficient recombination when dif SL sites are located on chromosome dimers. Moreover, the XerS/dif SL recombination requires the streptococcal protein FtsKSL, probably without the need for direct protein-protein interaction, which we demonstrated to be located at the division septum of Lactococcus lactis. Acquisition of the XerS recombination module can be considered as a landmark of the separation of Streptococci/Lactococci from other firmicutes and support the view that Xer recombination is a conserved cellular function in bacteria, but that can be achieved by functional analogs.

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“…This step strictly requires FtsKγ [Yates et al, 2003. Consistently, FtsKγ can activate recombination in the absence of FtsKαβ [Grainge et al, 2011;Nolivos et al, 2010].…”

Section: Activating Xer Recombinationmentioning

confidence: 62%

“…It can be further subdivided into α-, β-and γ-subdomains ( fig. 1 ) [Yates et al, 2003]. The α-and β-subdomains form the DNA pump [Massey et al, 2006], while the γ-subdomain acts as a control module .…”

mentioning

confidence: 99%

The FtsK Family of DNA Translocases Finds the Ends of Circles

et al. 2014

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A global view of bacterial chromosome choreography during the cell cycle is emerging, highlighting as a next challenge the description of the molecular mechanisms and factors involved. Here, we review one such factor, the FtsK family of DNA translocases. FtsK is a powerful and fast translocase that reads chromosome polarity. It couples segregation of the chromosome with cell division and controls the last steps of segregation in time and space. The second model protein of the family SpoIIIE acts in the transfer of the Bacillus subtilis chromosome during sporulation. This review focuses on the molecular mechanisms used by FtsK and SpoIIIE to segregate chromosomes with emphasis on the latest advances and open questions.

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Species specificity in the activation of Xer recombination at dif by FtsK

Cited by 52 publications

References 39 publications

FtsK translocation permits discrimination between an endogenous and an imported Xer/ dif recombination complex

FtsK translocation permits discrimination between an endogenous and an imported Xer/ dif recombination complex

The Unconventional Xer Recombination Machinery of Streptococci/Lactococci

The FtsK Family of DNA Translocases Finds the Ends of Circles

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