Species‐specific TaqMan probes for simultaneous identification of (Gadus morhua L.), haddock (Melanogrammus aeglefinus L.) and whiting (Merlangius merlangus L.).

Taylor, Martin I.; Fox, Clive; Rico, I.; Rico, Ciro

doi:10.1046/j.1471-8286.2002.00269.x

Cited by 100 publications

(89 citation statements)

References 3 publications

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“…In this approach, either a taxon-specific DNA-probe is developed (e.g. Taylor et al 2002) or cloning or pyro-sequencing are used to evaluate the presence of a broader range of taxa using DNA sequences (e.g. Cytochrome Oxidase I [COI] barcodes).…”

Section: Introductionmentioning

confidence: 99%

Identification of marine fish egg predators using molecular probes

Fox¹,

Taylor²,

Kooij³

et al. 2012

Mar. Ecol. Prog. Ser.

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Mortality during the egg and larval stages is thought to play a major role in de termining year-class strength of many marine fish. Predation of eggs and larvae is normally considered to be a major factor but the full suite of predators responsible has rarely been identified. Potential predators on a patch of plaice Pleuronectes platessa eggs located in the eastern Irish Sea were mapped using acoustics and sampled by trawl and a plankton multi-net. Gut contents of 3373 fish, crustacea and cephalopods sampled in the area were screened using a plaice-specific TaqMan DNA probe. Herring Clupea harengus and sprat Sprattus sprattus dominated trawl catches and showed high positive TaqMan responses (77 and 75% of individuals tested respectively). Locations of clupeid schools also broadly corresponded with the distribution of fish eggs in the plankton. Whiting Merlangius merlangus were also reasonably abundant in trawl hauls and 86% of their stomachs tested positive for plaice DNA. Species showing lower levels of positive TaqMan response included mackerel Scomber scombrus, poor cod Trisopterus minutus, squid Loligo spp., dogfish Scyliorhinus canicula and weever Trachinus vipera. Nonre activity of all negative controls precluded the occurrence of cross-contamination, and positive reactions from demersal species, such as dogfish and weever, may have resulted from secondary predation. No benthic macro-crustaceans tested positive. Samples of planktonic organisms yielded 13% positive TaqMan reactions, mainly from clupeoid or sandeel (Ammodytidae) larvae, but also included some Malacostraca and Amphipoda. Use of the molecular approach allowed rapid screening of a large number of potential predators of plaice eggs, and the results provide a more holistic description of the predator community than has previously been achieved.

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Section: Introductionmentioning

confidence: 99%

Identification of marine fish egg predators using molecular probes

Fox¹,

Taylor²,

Kooij³

et al. 2012

Mar. Ecol. Prog. Ser.

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“…Since the 5S rDNA is highly repeated in the genome and the NTS is quite short, the PCR amplification of a small amount of DNA is extremely robust, particularly if compared with single copy genes. mtDNA markers are commonly employed for species identification [9,12,13]. As most of the mtDNA genome is coding, between species differentiation is often based on single nucleotide polymorphism.…”

Section: Discussionmentioning

confidence: 99%

“…After reading relevant information about demersal fish stock declines [Ref. 4 and others] and the utility of molecular markers for stock assessment [3,9], a final discussion may be carried out concerning the application of molecular techniques in fisheries management. A final additional 2-h session could be convenient for this purpose.…”

Section: ) and Af335598 (M Merluccius)mentioning

confidence: 99%

Identification of highly prized commercial fish using a PCR‐based methodology

Morán

García‐Vázquez

2006

Biochem Molecular Bio Educ

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We report a practical class designed for undergraduate students of Marine Sciences as a part of the Genetics course. The class can also be included in undergraduate studies of food technology. The exercise was designed to emphasize the application of molecular biology techniques to fish species authentication and traceability. After a simple and rapid protocol for DNA extraction, PCR was used to analyze variation in size of individual 5S ribosomal gene spacer sequences in eight commercially important fishes. The resulting fragments containing these sequences were separated on agarose gels and visualized under a UV lamp. Differences in fragment length can be used to differentiate between species. This method is particularly useful in the identification of larvae, eggs, and processed food including frozen and canned products.

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“…The method was also used in heat and vacuum treated foodstuffs, however, without quantification (Hird et al, 2005). Taylor et al (2002) identified three commercially significant gadoid species (Gadus morhua, Melanogrammus aeglefinus and Merlangius merlangus) using the multiplex (TaqMan) PCR method based on variation in a 103-bp region of the ATP synthase subunit six (ATPase6) and eight (ATPase8) genes.…”

Section: Real-time Pcrmentioning

confidence: 99%

Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: a review

Hubálková¹,

Králík²,

Tremlová³

et al. 2007

Vet. Med. - Czech

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ABSTRACT:The purpose of the present study was to give an overview of imported and traded gadoid fish species (Gadiformes) in the Czech Republic and to describe available methods for their authentication. Due to the increasing interest of customers in the purchase of buy fish meat and other seafood animals, it is necessary to have available analytical methods with discriminating power of respective fish species. With regard to different values and prices of various fish species, these may be adultered. Until recently, electrophoretic, chromatographic and immunological methods based on the analysis of proteins extracted from fish musculature seemed to be promising. Using these methods, various fish species can be identified in fresh, chilled and frozen products. However, they often fail in heat treated products. Molecular biology methods based on DNA analysis are more reliable and suitable for the analysis of fish products that have been heat treated during the production process.Keywords: cod fish; fish species identification; electrophoresis; PCR-RFLP; cytochrome b gene; food adulteration List of abbreviations AK = adenylate kinase; ARGK = arginine kinase; ATP = adenosine triphosphate; CE = capillary electrophoresis; CK = creatine kinase; CR = Czech Republic; DGGE = denaturating gradient gel electrophoresis; DHA = docosahexaen acid; DNA = deoxyribonucleotide acid; EPA = eicosapentaen acid; EU = European Union; FINS = forensically informative nucleotide sequencing; G 3-PD = glycerol 3-phosphate dehydrogenase; HPLC = high performance liquid chromatography; IEF = isoelectric focusing; LDH = lactate dehydrogenase; MDH = malate dehydrogenase; MS = mass spectrometry; mt cyt b = mitochondrial cytochrome b gene; Mw = molecular weight; NDKA = nucleoside diphosphate kinase A; NTSs = nontranscribed spacers; PCR = polymerase chain reaction; PCR-RAPD = polymerase chain reaction -random amplified polymorphic DNA; PCR-RFLP = polymerase chain reaction -restriction fragment length polymorphism; PCR-SSCP = polymerase chain reaction -single strand conformation polymorphism; pI = isoelectric point; RP-HPLC = reversed phased -high performance liquid chromatography; SDS-PAGE = sodium dodecyl sulphate -polyacrylamide gel electrophoresis; urea-IEF = urea isoelectric focusing; 2DE = two dimensional electrophoresis

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Species‐specific TaqMan probes for simultaneous identification of (Gadus morhua L.), haddock (Melanogrammus aeglefinus L.) and whiting (Merlangius merlangus L.).

Cited by 100 publications

References 3 publications

Identification of marine fish egg predators using molecular probes

Identification of marine fish egg predators using molecular probes

Identification of highly prized commercial fish using a PCR‐based methodology

Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: a review

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