volume 66, issue 6, P3846-3859 1992
DOI: 10.1128/jvi.66.6.3846-3859.1992
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T Patschinsky, U Knippschild, W Deppert

Abstract: We have analyzed in detail the phosphorylation of p53 from normal (3T3) and simian virus 40 (SV40)transformed (SV3T3) BALB/c mouse cells and from normal (Fill) and SV40-transformed [FR(wt648)] rat cells by two-dimensional tryptic peptide mapping and phosphoamino acid analyses. To accommodate the different half-lives of p53 in normal (half-life, 15 min) and transformed (half-life, 20 h) cells and possible differences in the rates of turnover of phosphate at specific sites, cells were labeled for 2 h (short-term…

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