Species diversity of vaginal lactobacilli in norm and in dysbiotic states

Budilovskaya, Olga V.; Shipitsyna, Elena; Gerasimova, Ekaterina N.; Safronova, M. M.; Savicheva, Alevtina M.

doi:10.17816/jowd66224-32

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Different types of lactobacilli play an important role in maintaining the constancy of the vaginal microflora, but their share in this does not have the same degree. The dominance of Lactobacillus crispatus in the lactoflora is associated with the normal state of the vaginal microbiota, whereas the proportion of Lactobacillus iners-dominated samples in the vaginal lactoflora is higher in dysbiotic processes [1]. The question of the protective capacity of Lactobacillus iners remains controversial.…”

Section: Resultsmentioning

confidence: 99%

“…In bacterial vaginosis, the number of lactobacilli producing hydrogen peroxide significantly decreases or they are completely absent [1], polymicrobial, mainly anaerobic microflora prevails (the proportion of Gardnerella vaginalis, Mycoplasma hominis, Mobiluncus spp. increases), the overall degree of colonization of the vagina by various microorganisms increases (up to 109-1011 CFU/ml), the pH of the vaginal discharge increases (more than 4.5) [2].…”

Section: Resultsmentioning

confidence: 99%

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Clinical And Epidemiological Features Of Bacterial Vaginosis

M.M.¹

2020

TAJMSPR

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The review article presents the epidemiological characteristics of bacterial vaginosis, data on the lability of the composition of the vaginal microflora, including the species number of lactobacilli, depending on the ethnicity of the woman and the geographical area of residence. Literature data on factors affecting the composition of vaginal microbiocenosis, the most frequently detected types of bacteria in bacterial vaginosis, are presented.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Clinical And Epidemiological Features Of Bacterial Vaginosis

M.M.¹

2020

TAJMSPR

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“…Lactobacilli are a marker of the normal microbiocenosis of the genital tract. It is lactobacilli that determine the degree of colonization resistance of the vagina [8,9,15,16].…”

Section: Introductionmentioning

confidence: 99%

Features of vaginal microbiocenosis in women of reproductive age with overweight and obesity

et al. 2021

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Overweight and obesity play a negative role in gynecological and obstetric practicE.In women, the frequency of infectious pathology increases against the background of metabolic disorderS.The most common form of infectious vaginitis is bacterial urogenital candidiasis, in the etiological structure of which a significant role belongs to the fungi Candida albicans, as well as Candida non-albicans: C.glabrata, C.tropicalis, C.parapsilosis, C.krusei. Associations of Candida fungi with various representatives of opportunistic microflora, such as gram-positive and gram-negative aerobic, facultative-anaerobic and obligate-anaerobic microorganisms, are often formed. As a result, numerous bacterial pathogens multiply and the number of lactobacilli, which are usually part of the bacterial flora of the vagina, is significantly reduced. In bacterial vaginosis (BV), the concentration of anaerobic pathogens Peptostreptococcus sp, Gardnerella vaginalis, Peptostreptococcus Mobiluncus sp, Mycoplasma hominis can increase 100 timeS.Activation of Atopobium vaginae and Gardnerella vaginalis, which play a “key” role in the pathogenesis of BV, has been proven. The aim of the study was to study changes in the vaginal microbiome in women with candidiasis and bacterial vaginosis in order to improve existing treatment regimenS.We examined 120 women of reproductive age with overweight and obesity. The degree of microbial contamination was determined and the maximum possible spectrum of aerobic and facultative-anaerobic microflora was detected. In women with vulvovaginal candidiasis, overweight and obesity, a high concentration (lg5.8 CFU/ml) of Candida fungi was found, and in 95% of patients two-, three- and four-component associations of Candida fungi with various representatives of conditional pathogenic microflora. Lactobacillus deficiency was found in 58.3% of patients, and their complete absence – in 10.0%. Bacteriological examination of the vaginal contents of women with vaginosis and obesity revealed significant dysbiotic disorders of the vaginal microflora, three-, four- and even five-component associations of anaerobic and facultative anaerobic microflora with a predominance of anaerobeS.A low seeding level of lactobacilli (lg2.2 CFU/ml) was established. Thus, the gram-positive anaerobic and facultative anaerobic microflora of Firmicutes have a significant share in the spectrum of vaginal microflora in overweight and obese patients, in contrast to non-obese women of reproductive agE.In women of reproductive age with vulvovaginal candidiasis and obesity, in contrast to non-obese patients, a higher frequency of fungal-bacterial associations, a higher quantitative level of vaginal contamination by Candida albicans and non-albicans with a lack or general absence of lactoflora.

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State of the vaginal biocenosis depending on the species diversity of lactobacilli

Fedorova,

Abramovskikh,

Savochkina

et al. 2024

Russian Journal of Infection and Immunity

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Bacterial vaginosis is one of the most common reproductive health problems in women. Lactobacillus spp. play a leading role in the vaginal biocenosis and provide local antimicrobial defense, but not all species are equally capable of protecting the vaginal ecosystem from pathogens. Diagnostic methods in practical healthcare do not allow identification of Lactobacillus spp. species, which limits the ability to comprehensively assess the status of the vaginal biocenosis. We conducted a study in which we compared microscopy data of the examined smear with the detection of Lactobacillus spp. and other microorganisms. The aim of the study was to evaluate the species composition of Lactobacillus spp. from the vagina depending on morphologic characteristics and composition of associated flora. The study included 64 conditionally healthy women of reproductive age from 18 to 35 years old. The study of species composition of the vaginal biocenosis and typing of lactobacilli was carried out by real-time PCR methods. The obtained results were processed using generally accepted methods of descriptive and ordinal statistics. During the study, it was found that the vaginal biocenosis was dominated by species of Lactobacillus spp. L. crispatus, L. vaginalis, L. iners and L. jensenii. L. johnsonii was rarely found, and L. acidophilus was not detected. Typing results showed that the vagina was more frequently colonized by more than one species of Lactobacillus spp. while a single species of Lactobacillus spp. was detected less frequently. A correlation was found between Lactobacillus spp. species and bacilli thickness. If thick bacilli are detected in gynecological smear microscopy, they belong to the species L. crispatus, which has high colonization resistance and is a factor in the stability of the vaginal biocenosis. If smear microscopy reveals thin bacilli, then these are species of L. iners and L. jensenii. These representatives of Lactobacillus spp. are not directly related to the dysbiotic state of the vagina, but may indicate the possible development of dysbiosis in the future.

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Species diversity of vaginal lactobacilli in norm and in dysbiotic states

Cited by 6 publications

References 14 publications

Clinical And Epidemiological Features Of Bacterial Vaginosis

Clinical And Epidemiological Features Of Bacterial Vaginosis

Features of vaginal microbiocenosis in women of reproductive age with overweight and obesity

State of the vaginal biocenosis depending on the species diversity of lactobacilli

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