Species and serovars of the genus Listeria lsolated from different sources in Brazil from 1971 to 1997

Hofer, Ernesto; Ribeiro, R.; Feitosa, Deise Paranhos

doi:10.1590/s0074-02762000000500005

Cited by 47 publications

(36 citation statements)

References 18 publications

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“…The importance of this work from the public health view point is that it shows that a potential risk to the health of the general population, heightened by the increase in the number of elderly and the immunodepressed for whom L. monocytogenes is potentially serious and also by the fact that L. monocytogenes isolates which belong to serotype 1/ 2a were found among those extracted. Furthermore, this is one of the three serotypes most commonly responsible for outbreaks of listeriosis (1/2a, 1/ 2 b, the most frequent being 4 b) (Kerouanton et al, 1998;Margolles et al, 1998;Hofer et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Listeria monocytogenes in cheese and ice cream produced in the State of Paraná, Brazil

Abrahão¹,

Abrahão²,

Monteiro³

et al. 2008

Rev. Bras. Cienc. Farm.

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Section: Resultsmentioning

confidence: 99%

Occurrence of Listeria monocytogenes in cheese and ice cream produced in the State of Paraná, Brazil

Abrahão¹,

Abrahão²,

Monteiro³

et al. 2008

Rev. Bras. Cienc. Farm.

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“…Hofer et al [15], evaluated the distribution and frequency of Listeria species and serotypes from different areas of Brazil, isolated from various sources of infection and transmission vehicles during the period from 1971 to 1997, totaling 3,112 strains. The authors found that the occurence of L. monocytogenes serotypes was usually limited to 4b, 1/2a and 1/2b, with prevalence of 4b in isolates from human and meat/fish sources.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, previous studies described a high ocurrence of 4b serotype in human and foods specimens [14][15][16][17][18]. In an extensive study, Hofer et al [14] observed the prevalence of the 4b serotype in meat products and fish from different sources in Brazil, followed by 1/2a and 1/2b serotypes.…”

Section: Introductionmentioning

confidence: 99%

Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Barbosa

Cerqueira

Rusak

et al. 2015

J Infect Dev Ctries

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Introduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs). Methodology: We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis. Results: ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECIIpositive; and two ECI strains from a human case (1982) and from bovine meat (2009). Conclusions: The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings.

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“…We examined transfer of such resistance among L. innocua and L. welshimeri as well as from these non-pathogenic species to L. monocytogenes. Listeria (104,105,168,197). We recently provided evidence suggesting that non-pathogenic Listeriae may constitute reservoirs for resistance to the quaternary ammonium disinfectant benzalkonium chloride (BC), being able to conjugatively disseminate resistance to BC to other Listeriae, including L. monocytogenes (117).…”

Section: Such Findings Suggest the Need To Characterize Horizontal Trmentioning

confidence: 99%

“…Furthermore, a number of studies have suggested cross-resistance between BC and antibiotics (182,187,216). and L. welshimeri have been found to be more common than L. monocytogenes in food processing environments and in foods, and to grow faster than L. monocytogenes in foods and other media (8,47,104,140,168,197,201,221). They are also more likely than L.…”

mentioning

confidence: 99%

Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

Rakić-Martinez¹

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ACKNOWLEDGMENTSI would like to thank Dr. Vera Katić for being my mentor, not only through PhD studies, but in my undergraduate years as well. Her objectivity, articulate thinking and dedication to work inspired me to keep moving forward even after long, tiresome days in the lab. I will forever appreciate Dr. Katic's encouragement to pursue personal happiness and her support in important life decisions.I am forever grateful to Dr. Sophia Kathariou for accepting me in her lab and making me feel at home while far from it. Her incredible ability to find the best solutions when things were not looking promising and open completely new horizons along with her creativity and knowledge made science so attractive. Looking at all the great, bright and skilful young people pursuing their dreams under Dr. Kathariou's guidance makes me very proud for being a part of it.My appreciation also goes to Dr. Lepšanović and Dr. Mišić for accepting to be members of the committee. I am thankful to all my lab mates and to Ms. Robin Siletzky for her solid support throughout my work. A special thanks to Dr. Vikrant Dutta for his patience, willingness to help and his much admired teaching skills.Lastly and foremost I would like to thank my family. To my dear parents Ljubo and Zora whose unconditional love, support and presence whenever I needed them have been and will always be my motive to be better. To my big brother Zoran, who has been, and is my role model, but still owes me German lessons. Finally, I want to thank my boys, my beloved husband Sergio and my precious son Gregory, who have been an endless source of energy, support and love. Thanks for being my wingmen guys! ABSTRACTAbility to contaminate ready-to-eat, highly processed, cold-stored food products, relatively high fatality rate and environmental adaptations define .Listeria monocytogenes as a challenging foodborne pathogen of high public health significance. Listeria's adaptations and prolonged persistence in food processing facilities are a key link to contamination of foods.In recent years, bacterial multidrug resistance has been recognized as an emerging health challenge. Exposure to antimicrobials (antibiotics, disinfectants or environmentally ubiquitous heavy metals), may provide selective pressures for resistance with accompanying cross-resistance and increase in environmental fitness.In this work I addressed some of the possible mechanisms of adaptations that may contribute to persistence of L. monocytogenes in the environment as well as to survival and growth in different foods. This includes adaptations to heavy metals such as cadmium, disinfectants such as benzalkonium chloride (BC), selected antibiotics (e.g. the fluoroquinolone ciprofloxacin) and other toxic compounds (e.g. tetraphenyl-phosponium, often used as an anti-carcinogenic and efflux pump substrate). In order to characterize the impact of multidrug resistance on survival and growth of L. monocytogenes in different foods, we tested mutants adapted to higher concentrations of antibiotic ciprofloxacin and ...

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Species and serovars of the genus Listeria lsolated from different sources in Brazil from 1971 to 1997

Abstract: (352-11.3%), 1/2a (162-5.2%), and 1/2b (148-4.7%). The predominant serovar in L. innocua was 6a (2,093-67.2%). Considerations about laboratory methods for diagnosis and epidemiological aspects are presented on the basis of the results obtained.

Cited by 47 publications

References 18 publications

Occurrence of Listeria monocytogenes in cheese and ice cream produced in the State of Paraná, Brazil

Occurrence of Listeria monocytogenes in cheese and ice cream produced in the State of Paraná, Brazil

Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

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