Speciation of Se, Fe and Zn in Human Milk Whey: The use of Instrumental Neutron Activation Analysis (INAA) to corroborate element profiles measured with Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES)

Br�tter, V. E. Negretti de; Recknagel, Sebastian; Gawlik, D.

doi:10.1007/bf00322947

Cited by 38 publications

(34 citation statements)

References 11 publications

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“…Serum, milk and urine samples were measured after dilution with 1% (v/v) nitric acid suprapur (Merck, Darmstadt, Germany), whereas RBC and feces samples were first digested with nitric acid/ hydrogen peroxide ultrapur (Merck, Darmstadt, Germany) before final dilution. Independent analytical methods and certified reference materials were used for quality control (Nuttal et al, 1995;Negretti de Brätter et al, 1995). Se values of the materials, measured for quality control, were in good agreement with the certified values, with a day-to-day and in-run precision above 95%.…”

Section: Sample Collection and Analysismentioning

confidence: 94%

Selenium status in term and preterm infants during the first months of life

et al. 2007

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Objective: We hypothesized that very low birth weight (VLBW) infants have reduced serum and red blood cell (RBC) selenium (Se) at birth, which decrease further with current nutrition and are associated with chronic lung disease and septicaemia. Design: We studied Se intake, concentration in serum and RBCs and glutathione peroxidase (GSH-Px) activity in preterm and term infants from birth until 16 weeks. Data are mean7standard deviation (s.d.). Setting: Seventy-two preterm infants in two groups, born in Berlin, gestational age 26 þ 0/30 þ 0 weeks, birth weight 845/ 1270 g, with low Se intake (2.270.8/2.571.2 mg/kg/day), and 55 term infants, gestational age 39 þ 1 weeks, birth weight 3160 g, born in Venezuela (high Se intake: 2978 mg/day). Results: A balance study in 10 preterm infants showed that Se is well absorbed from human milk (7779%). Serum concentration was higher in term (142.0740.0 mg/l) than in preterm infants (17.878.1/19.972.2 mg/l) at 4/7 weeks. Serum and RBC concentration of Se declined in all infants, low values in preterm infants did not correlate with chronic lung disease and septicaemia. GSH-Px activity in RBCs remained stable until 6 weeks of age in all infants and was not correlated with Se in RBCs. Conclusions: Se concentration in serum decreases during the first weeks of life and depends on intake. GSH-Px activity is not useful as a marker for Se status in infants up to 16 weeks after birth.

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Section: Sample Collection and Analysismentioning

confidence: 94%

Selenium status in term and preterm infants during the first months of life

et al. 2007

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“…Michalke & Schramel (1998) and Michalke (2000) calculated a mass balance (sum of Se species:total Se) in breast milk of 94 -100 %. However, in seleniferous areas of Venezuela, breast-milk samples with very high Se concentrations (1240 -1930 mg/kg DM) showed a mass balance of 87 % corresponding to four peaks in the whey fraction (Negretti de Bratter et al 1995) and a unique selenoprotein pattern that differed from that of cows' milk and soyabean-formula (Bratter et al 1998).…”

Section: Selenium Species In Breast Milkmentioning

confidence: 99%

Selenium and breast-feeding

Dórea

2002

Br J Nutr

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The objective of the present review is to discuss Se nutrition during breast-feeding, encompassing environmental and maternal constitutional factors affecting breast-milk-Se metabolism and secretion. A literature search of Medline and Webofscience was used to retrieve and select papers dealing with Se and breast milk. Although Se in natural foods occurs only in organic form, breast milk responds to organic and inorganic Se in supplements. Inorganic Se (selenite, selenate), which is largely used in maternal supplements, is not detectable in breast milk. The mammary-gland regulating mechanism controls the synthesis and secretion of seleno-compounds throughout lactation, with a high total Se level in colostrum that decreases as lactation progresses. Se appears in breast milk as a component of specific seleno-proteins and selenoamino-acids in milk proteins that are well tolerated by breast-fed infants even in high amounts. Se in breast milk occurs as glutathione peroxidase (4 -32 % total Se) . selenocystamine . selenocystine . selenomethionine. The wide range of breast-milk Se concentrations depends on Se consumed in natural foods, which reflects the Se content of the soils where they are grown. Se prophylaxis, either through soil Se fertilization or maternal supplements, is effective in raising breast-milk Se concentration. In spite of wide variation, the median Se concentration from studies worldwide are 26, 18, 15, and 17 mg/l in colostrum (0 -5 d), transitional milk (6 -21 d), mature milk (1 -3 months) and late lactation (.5 months) respectively. Se recommendations for infants are presently not achieved in 30 % of the reported breast-milk Se concentrations; nevertheless Se status is greater in breast-fed than in formula-fed infants.

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“…Differences were also obtained between the subcellular fractions indicating that some selenoproteins are involved in different intracellular processes [12,41]. In human breast milk four selenium species with the apparent molecular weights of 15, 60, 1500 and >2000 kDa were detected [42].…”

Section: Species Of Interestmentioning

confidence: 98%

“…SE HPLC-ICP MS of a human serum sample yielded three signals, none of which, however, co-eluted with the glutathione peroxidase activity [38]. SE HPLC of human breast milk whey produced four Se signals corresponding to species with apparent molecular weights of 15, 60, 1500 and >2000 kDa [42].…”

Section: Detection Of Selenoproteins By Hplc-icp Msmentioning

confidence: 98%

Species-selective determination of selenium compounds in biological materials (Technical Report)

Łobiński

Edmonds

Suzuki

et al. 2000

Pure and Applied Chemistry

157

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Republication or reproduction of this report or its storage and/or dissemination by electronic means is permitted without the need for formal IUPAC permission on condition that an acknowledgmentMembership of the Commission during the preparation of this report (1997)(1998)(1999) Species-selective determination of selenium compounds in biological materials (Technical Report)Abstract: There is substantial evidence of the complexity of selenium speciation in living organisms and of the importance of the selective determination of the particular species of this element in order to understand its metabolism and biological significance in clinical chemistry, biology, toxicology, and nutrition. The state-of-the-art of analytical techniques available for this purpose is critically evaluated with particular emphasis on the element-selective detection and identification of the detected selenium compounds. Whereas there are a number of techniques available that are able to detect various selenium species in living organisms selectively, few techniques exist that are able to identify and to characterize the species detected.

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Speciation of Se, Fe and Zn in Human Milk Whey: The use of Instrumental Neutron Activation Analysis (INAA) to corroborate element profiles measured with Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES)

Cited by 38 publications

References 11 publications

Selenium status in term and preterm infants during the first months of life

Selenium status in term and preterm infants during the first months of life

Selenium and breast-feeding

Species-selective determination of selenium compounds in biological materials (Technical Report)

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