Specialized pro-resolving receptors are expressed in salivary glands with Sjögren's syndrome

Santos, Harim Tavares dos; Nam, Ki Woong; Maslow, Frank; Trump, Bryan; Baker, Olga J.

doi:10.1016/j.anndiagpath.2021.151865

Cited by 6 publications

(5 citation statements)

References 126 publications

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“…This accords with the finding of FPR2/ALX expression in human dermal epithelium [7]. Other epithelial cells expressing FPR2/ALX include ductal and acinar cells in human minor salivary glands [26][27][28], as well as cultured human submandibular gland cells [26]. In cultured oral epithelial cells, qRT-PCR showed transcription of FPR2/ALX and flow cytometry showed that the receptor was present on both the cell surface and within the cytoplasm.…”

Section: Discussionsupporting

confidence: 88%

Expression and function of resolvin RvD1_{n‐3 DPA} receptors in oral epithelial cells

Balta

Schreurs

Hansen

et al. 2022

European J Oral Sciences

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Chronic inflammatory responses can inflict permanent damage to host tissues. Specialized pro-resolving mediators downregulate inflammation but also can have other functions. The aim of this study was to examine whether oral epithelial cells express the receptors FPR2/ALX and DRV1/GPR32, which bind RvD1 n-3 DPA , a recently described pro-resolving mediator derived from omega-3 docosapentaenoic acid (DPA), and whether RvD1 n-3 DPA exposure induced significant responses in these cells. Gingival biopsies were stained using antibodies to FPR2/ALX and DRV1/GPR32. Expression of FPR2/ALX and DRV1/GPR32 was examined in primary oral epithelial cells by qRT-PCR, flow cytometry, and immunofluorescence. The effect of RvD1 n-3 DPA on intracellular calcium mobilization and transcription of beta-defensins 1 and 2, and cathelicidin was evaluated by qRT-PCR. FPR2/ALX and DRV1/GPR32 were expressed by gingival keratinocytes in situ. In cultured oral epithelial cells, FPR2/ALX was detected on the cell surface, whereas FPR2/ALX and DRV1/GPR32 were detected intracellularly. Exposure to RvD1 n-3 DPA induced intracellular calcium mobilization, FPR2/ALX internalization, DRV1/GPR32 translocation to the nucleus, and significantly increased expression of genes coding for beta-defensin 1, beta-defensin 2, and cathelicidin. This shows that the signal constituted by RvD1 n-3 DPA is recognized by oral keratinocytes and that this can strengthen the antimicrobial and regulatory potential of the oral epithelium.

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Section: Discussionsupporting

confidence: 88%

Expression and function of resolvin RvD1_{n‐3 DPA} receptors in oral epithelial cells

Balta

Schreurs

Hansen

et al. 2022

European J Oral Sciences

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“…RvD1, 17R-RvD1, RvD3, 17R-RvD3, RvD5, RvE1, and 18S-RvE1 can combine with the G protein-coupled receptor (GPR32) expressed in monocyte-macrophages, lymphocytes, endothelial cells, and vascular smooth muscle cells, while GPR18/DRV2 is a novel discovered receptor of RvD2 expressed in macrophages, neutrophils, and monocytes [ 55 , 56 ]. In addition, RvD1, 17R-RvD1, and RvD3 can also combine with the G protein-coupled formyl peptide receptor 2 (ALX/FPR2) to produce anti-inflammatory effects [ 57 , 58 , 59 ]. Leukotriene B4 receptor 1 (BLT1) expressed in lymphocytes and leukocytes and ChemR23 expressed in NK cells, macrophages, epithelial cells, and dendritic cells have been proven to be the receptors of RvE1, 18S-RvE1, and RvE2 through target screening [ 60 , 61 ].…”

Section: Resolvins Receptorsmentioning

confidence: 99%

Roles of Resolvins in Chronic Inflammatory Response

Liu

Fan

Liu

et al. 2022

IJMS

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An inflammatory response is beneficial to the organism, while an excessive uncontrolled inflammatory response can lead to the nonspecific killing of tissue cells. Therefore, promoting the resolution of inflammation is an important mechanism for protecting an organism suffering from chronic inflammatory diseases. Resolvins are a series of endogenous lipid mediums and have the functions of inhibiting a leukocyte infiltration, increasing macrophagocyte phagocytosis, regulating cytokines, and alleviating inflammatory pain. By promoting the inflammation resolution, resolvins play an irreplaceable role throughout the pathological process of some joint inflammation, neuroinflammation, vascular inflammation, and tissue inflammation. Although a large number of experiments have been conducted to study different subtypes of resolvins in different directions, the differences in the action targets between the different subtypes are rarely compared. Hence, this paper reviews the generation of resolvins, the characteristics of resolvins, and the actions of resolvins under a chronic inflammatory response and clinical translation of resolvins for the treatment of chronic inflammatory diseases.

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“…However, when this mechanism is abnormal, dead cells are not cleared in time, leading to the formation of their antigens, increased levels of cytokines and chemokines, and lymphocyte infiltration ( 155 ). Specific pro-resolving mediators (SPMs, including liposomes, resolvins, marisins, and protectin) and their aspirin-triggered (AT) forms act as inflammatory mediators, promoting tissue regeneration by limiting uncontrolled inflammation while promoting its termination ( 156 ). Odusanwo et al.…”

Section: Regulation Of the Immune Microenvironmentmentioning

confidence: 99%

“…Resolvins are special proresolving mediators (SPMs) that can actively regulate inflammation. Furthermore, the expression of various SPM receptors (ALX/FPR2, BLT1, and CMKLR1) was found in human salivary glands, which may be a potential target for treating pSS patients ( 156 ).…”

Section: Treatmentmentioning

confidence: 99%

Composition and regulation of the immune microenvironment of salivary gland in Sjögren’s syndrome

Tan

Wang

2022

Front. Immunol.

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Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease characterized by exocrine gland dysfunction and inflammation. Patients often have dry mouth and dry eye symptoms, which seriously affect their lives. Improving dry mouth and eye symptoms has become a common demand from patients. For this reason, researchers have conducted many studies on external secretory glands. In this paper, we summarize recent studies on the salivary glands of pSS patients from the perspective of the immune microenvironment. These studies showed that hypoxia, senescence, and chronic inflammation are the essential characteristics of the salivary gland immune microenvironment. In the SG of pSS, genes related to lymphocyte chemotaxis, antigen presentation, and lymphocyte activation are upregulated. Interferon (IFN)-related genes, DNA methylation, sRNA downregulation, and mitochondrial-related differentially expressed genes are also involved in forming the immune microenvironment of pSS, while multiple signaling pathways are involved in regulation. We further elucidated the regulation of the salivary gland immune microenvironment in pSS and relevant, targeted treatments.

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Specialized pro-resolving receptors are expressed in salivary glands with Sjögren's syndrome

Cited by 6 publications

References 126 publications

Expression and function of resolvin RvD1_{n‐3 DPA} receptors in oral epithelial cells

Expression and function of resolvin RvD1_{n‐3 DPA} receptors in oral epithelial cells

Roles of Resolvins in Chronic Inflammatory Response

Composition and regulation of the immune microenvironment of salivary gland in Sjögren’s syndrome

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Specialized pro-resolving receptors are expressed in salivary glands with Sjögren's syndrome

Cited by 6 publications

References 126 publications

Expression and function of resolvin RvD1n‐3 DPA receptors in oral epithelial cells

Expression and function of resolvin RvD1n‐3 DPA receptors in oral epithelial cells

Roles of Resolvins in Chronic Inflammatory Response

Composition and regulation of the immune microenvironment of salivary gland in Sjögren’s syndrome

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Expression and function of resolvin RvD1_{n‐3 DPA} receptors in oral epithelial cells

Expression and function of resolvin RvD1_{n‐3 DPA} receptors in oral epithelial cells