1995
DOI: 10.1021/bi00008a029 View full text |Buy / Rent full text
|
|

Abstract: An in vivo assay employing psoralen cross-linking was used to investigate the presence of unrestrained supercoiling in DNA sequences located in nontranscribed regions flanking the 3' ends of the pair of divergent heat shock protein 70 (hsp70) genes at locus 87A7 of Drosophila. Two of the regions examined contain sequences comprising the previously defined specialized chromatin structure elements (scs and scs'). Both of these putative chromosomal domain boundaries exhibited very similar levels of unrestrained n… Show more

Help me understand this report

Search citation statements

Order By: Relevance
Select...
2
2
16
0

Year Published

1999
1999
2004
2004

Publication Types

Select...
3

Relationship

1
2

Authors

Journals

2
16
0
Order By: Relevance
“…In particular, transcriptionally active DNA contains high levels of localized torsional tension, consistent with the observation that transcription in vivo results in the generation of a twin supercoiled domain with a positively and negatively supercoiled domain, respectively, in front and behind the transcription complex (20,30,32,41,66). Negative supercoiling, possibly due to the absence of canonical nucleosomes, has also been associated with DNase I-hypersensitive, transcriptionregulatory regions (20). Interestingly, a transcribed open reading frame has been detected in the context of AAVS1 (23), and we have recently demonstrated that a DNase I-hypersensitive site with transcriptional enhancer-like properties localizes immediately upstream of the RBS in AAVS1 (26).…”
Section: Discussionsupporting
See 1 more Smart Citation
Create an account to read the remaining citation statements from this report. You will also get access to:
  • Search over 1.2b+ citation statments to see what is being said about any topic in the research literature
  • Advanced Search to find publications that support or contrast your research
  • Citation reports and visualizations to easily see what publications are saying about each other
  • Browser extension to see Smart Citations wherever you read research
  • Dashboards to evaluate and keep track of groups of publications
  • Alerts to stay on top of citations as they happen
  • Automated reference checks to make sure you are citing reliable research in your manuscripts
  • 14 day free preview of our premium features.

Trusted by researchers and organizations around the world

Over 130,000 students researchers, and industry experts at use scite

See what students are saying

rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…In particular, transcriptionally active DNA contains high levels of localized torsional tension, consistent with the observation that transcription in vivo results in the generation of a twin supercoiled domain with a positively and negatively supercoiled domain, respectively, in front and behind the transcription complex (20,30,32,41,66). Negative supercoiling, possibly due to the absence of canonical nucleosomes, has also been associated with DNase I-hypersensitive, transcriptionregulatory regions (20). Interestingly, a transcribed open reading frame has been detected in the context of AAVS1 (23), and we have recently demonstrated that a DNase I-hypersensitive site with transcriptional enhancer-like properties localizes immediately upstream of the RBS in AAVS1 (26).…”
Section: Discussionsupporting
“…In spite of this, however, it is now very well established that localized regions of unrestrained supercoiling are present in the human chromatin (11,24,31,36). In particular, transcriptionally active DNA contains high levels of localized torsional tension, consistent with the observation that transcription in vivo results in the generation of a twin supercoiled domain with a positively and negatively supercoiled domain, respectively, in front and behind the transcription complex (20,30,32,41,66). Negative supercoiling, possibly due to the absence of canonical nucleosomes, has also been associated with DNase I-hypersensitive, transcriptionregulatory regions (20).…”
Section: Discussionmentioning
“…Each element contains two nuclease-hypersensitive sites flanking a nuclease-resistant core and topoisomerase II cleavage sites that redistribute upon heat shock (64, 65). These properties suggested that scs and scsЈ are boundary elements of the 87A puff domain that control the long-range topological transitions and regulate the limits of decondensation associated with transcription of this heat shock locus (33,64,65).Characterization of the functional properties of scs and scsЈ demonstrated that these sequences are insulators (12,15,34,35,56,66). Insulators are a class of regulatory elements that establish independent domains of transcriptional activity within eukaryotic genomes (19,36,67).…”
mentioning
“…Each element contains two nuclease-hypersensitive sites flanking a nuclease-resistant core and topoisomerase II cleavage sites that redistribute upon heat shock (64, 65). These properties suggested that scs and scsЈ are boundary elements of the 87A puff domain that control the long-range topological transitions and regulate the limits of decondensation associated with transcription of this heat shock locus (33,64,65).…”
mentioning
“…Topological domains, often referred to as chromosomal loops, have been detected in many organisms including Escherichia coli (1), Drosophila (2), and humans (3) (for review see Ref. 4).…”
mentioning