Spatio-temporal patterns in phytoplankton assemblages in inshore–offshore gradients using flow cytometry: A case study in the eastern English Channel

Bonato, Simon; Breton, Elsa; Didry, Morgane; Lizon, Fabrice; Cornille, Vincent; Lécuyer, Éric; Christaki, Urania; Artigas, Luis Felipe

doi:10.1016/j.jmarsys.2015.11.009

Cited by 18 publications

(9 citation statements)

References 58 publications

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“…For example, pigment‐based community analysis is able to identify chlorophytes as co‐dominating Arctic waters using chlorophyll b as a biomarker for this group (Fragoso et al ), whereas this group is not distinguishable using the CytoSense parameters. The colonial prymnesiophyte Phaeocystis pouchetii , which constitutes an important component of phytoplankton spring blooms in the West Greenland Current (eastern Labrador Sea) (Frajka‐Williams and Rhines ; Fragoso et al , ), has been previously detected using the CytoSense (Bonato et al , ). In this study, however, Phaeocystis colonies were not observed, potentially due to their low abundance in the Labrador Sea during the summer of June 2014 (Fragoso et al , ).…”

Section: Discussionmentioning

confidence: 99%

Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer

Fragoso

Poulton

Pratt

et al. 2019

Limnology & Oceanography

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Plankton are an extremely diverse and polyphyletic group, exhibiting a large range in morphological and physiological traits. Here, we apply automated optical techniques, provided by the pulse-shape recording automated flow cytometer-CytoSense-to investigate trait variability of phytoplankton and plastidic ciliates in Arctic and Atlantic waters of the subpolar North Atlantic. We used the bio-optical descriptors derived from the CytoSense (light scattering [forward and sideward] and fluorescence [red, yellow/green and orange from chlorophyll a, degraded pigments, and phycobiliproteins, respectively]) and translated them into functional traits to demonstrate ecological trait variability along an environmental gradient. Cell size was the master trait varying in this study, with large photosynthetic microplankton (> 20 μm in cell diameter), including diatoms as single cells and chains, as well as plastidic ciliates found in Arctic waters, while small-sized phytoplankton groups, such as the picoeukaryotes (< 4 μm) and the cyanobacteria Synechococcus were dominant in Atlantic waters. Morphological traits, such as chain/colony formation and structural complexity (i.e., cellular processes, setae, and internal vacuoles), appear to favor buoyancy in highly illuminated and stratified Arctic waters. In Atlantic waters, small cell size and spherical cell shape, in addition to photo-physiological traits, such as high internal pigmentation, offer chromatic adaptation for survival in the low nutrient and dynamic mixing waters of the Atlantic Ocean. The use of automated techniques that quantify ecological traits holds exciting new opportunities to unravel linkages between the structure and function of plankton communities and marine ecosystems.

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Section: Discussionmentioning

confidence: 99%

Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer

Fragoso

Poulton

Pratt

et al. 2019

Limnology & Oceanography

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“…This was because they had been previously observed by microscopy and/ or flow cytometry in the same area (e.g. Grattepanche et al 2011a,b, Monchy et al 2012, Bonato et al 2015, 2016. Conversely, for OTUs that were affiliated with taxonomic groups impossible to detect with microscopy, consideration of their annotation to higher taxonomic groups (e.g.…”

Section: Discussionmentioning

confidence: 99%

Small-scale variability of protistan planktonic communities relative to environmental pressures and biotic interactions at two adjacent coastal stations

Genitsaris¹,

Monchy²,

Breton³

et al. 2016

Mar. Ecol. Prog. Ser.

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The aim of this study was to analyze planktonic protistan assemblages under different environmental pressures at 2 adjacent coastal stations (inshore and offshore; ~6.4 km apart) in the eastern English Channel (EEC). Samples were collected between March 2012 and June 2013, and analyzed using bioinformatic analysis and tag pyrosequencing of the V2−V3 hypervariable region of the 18S rRNA gene. In addition to the taxonomic composition of the protistan communities, the detected operational taxonomic units (OTUs) were sorted into 6 major functional groups based on their trophic roles in marine systems. Comparisons of 13 environmental factors, including physical and chemical variables, indicated significant differences between the 2 stations. However, the only significant relationship between environmental pressures and protistan community structure was detected when the trophic role of the OTUs was considered at the inshore station. In order to interpret differences in community structure, the effect of biotic inter actions was investigated through the examination of the co-occurrence networks of the protistan communities at both stations. In terms of the number of edges and connectivity of nodes, the analysis showed that the inshore station had more complex associations between OTUs than the offshore station. This strongly suggests that due to the higher and more variable environmental pressures the inshore station comparatively receives, its protistan community has developed a greater complexity of biotic connections. This in turn reflects the rapid responses of trophic interactions within the entire microbial community.

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“…The Eastern English Channel (EEC) is a well-mixed mesoeutrophic sea undergoing multiple environmental disturbances such as temperature rise (McLean et al, 2019) and nutrient enrichment, mainly from the Seine and Somme rivers (Thieu et al, 2009) as a result of anthropogenic activities in the watersheds, especially intensive agriculture practices (e.g., Garnier et al, 2019). This nutrient enrichment is known to trigger the recurrent spring Phaeocystis blooms of EEC (Lancelot et al, 1987;Lancelot, 1995;Breton et al, 2000, Breton et al, 2017Grattepanche et al, 2011a,b;Bonato et al, 2016;Ménesguen et al, 2018). By affecting the diatom and copepod communities, two interacting key compartments of the ecosystem trophodynamics (Smetacek, 1999;Tréguer and Pondaven, 2000;Beaugrand et al, 2003;Kiørboe, 2011;Mitra et al, 2014;Steinberg and Landry, 2017), this prymnesiophyte can disrupt the structure of the food web (Schoemann et al, 2005;Nejstgaard et al, 2007), especially during the late winter/spring and summer/autumn periods (Gasparini et al, 2000;Antajan, 2004).…”

Section: Introductionmentioning

confidence: 99%

Seasonal Variations in the Biodiversity, Ecological Strategy, and Specialization of Diatoms and Copepods in a Coastal System With Phaeocystis Blooms: The Key Role of Trait Trade-Offs

et al. 2021

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Although eutrophication induced by anthropogenic nutrient enrichment is a driver of shifts in community composition and eventually a threat to marine biodiversity, the causes and consequences on ecosystem functioning remain greatly unknown. In this study, by applying a trait-based approach and measuring niche breadth of diatoms and copepods, the drivers and underlying mechanisms of the seasonal species succession of these ecological communities in a coastal system dominated in spring by Phaeocystis blooms were explored. It is suggested that the seasonal succession of diatoms and copepods is the result of several trade-offs among functional traits that are controlled by the seasonal abiotic and biotic pressure encountered by the plankton communities. The results of this study highlight that a trade-off between competition and predator, i.e., weak competitors are better protected against predation, plays an important role in promoting plankton species richness and triggers the Phaeocystis bloom. As often observed in eutrophicated ecosystems, only the biotic homogenization of the copepod community and the shift in the diet of copepods toward Phaeocystis detrital materials have been detected during the Phaeocystis bloom. The diatom and copepod communities respond synchronously to fluctuating resources and biotic conditions by successively selecting species with specific traits. This study confirms the key role of competition and predation in controlling annual plankton succession.

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Spatio-temporal patterns in phytoplankton assemblages in inshore–offshore gradients using flow cytometry: A case study in the eastern English Channel

Cited by 18 publications

References 58 publications

Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer

Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer

Small-scale variability of protistan planktonic communities relative to environmental pressures and biotic interactions at two adjacent coastal stations

Seasonal Variations in the Biodiversity, Ecological Strategy, and Specialization of Diatoms and Copepods in a Coastal System With Phaeocystis Blooms: The Key Role of Trait Trade-Offs

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