Spatial organization of biochemical cues in 3D-printed scaffolds to guide osteochondral tissue engineering

Camacho, Paula; Behre, Anne; Fainor, Matthew; Seims, Kelly B.; Chow, Lesley W.

doi:10.1039/d1bm00859e

Cited by 21 publications

(22 citation statements)

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“…Synthetic polymer hydrogels will play an integral role in the fabrication of such scaffolds, especially when processed with 3D printing. Spatially controlled biochemical cues that make the addition of differentiation factors unnecessary are considered a state-of-the-art approach …”

Section: Discussionmentioning

confidence: 99%

“…A triangular geometry was superior in terms of cell adhesion and proliferation of infrapatellar fat pad adipose MSCs . Besides those tailor-made polymers, commercially available thermoplastics are still studied, and scaffolds for cartilage TE with complex structures are prepared, especially with the contribution of 3D printing. ,, The ease of reproduction of porous scaffolds made from PCL or PLGA with a controlled, organized porous structure gives a unique feature to these polymers, as such organized structures are essential in mimicking the inherent complexity of osteochondral tissues. Finally, gradient multilayer scaffolds containing other compounds that improve osteogenesis, such as HA (Figure a), tricalcium phosphate (TCP) and decellularized ECM (dECM) (Figure b) or PCL conjugates with hyaluronic acid binding peptides derived from aggrecan, were prepared with 3D printing and could provide a valuable and reproducible solution to combat cartilage morbidity.…”

Section: Design Aspects Polymer Selection and Latest Advancesmentioning

confidence: 99%

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Biocompatible Synthetic Polymers for Tissue Engineering Purposes

et al. 2022

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Synthetic polymers have been an integral part of modern society since the early 1960s. Besides their most well-known applications to the public, such as packaging, construction, textiles and electronics, synthetic polymers have also revolutionized the field of medicine. Starting with the first plastic syringe developed in 1955 to the complex polymeric materials used in the regeneration of tissues, their contributions have never been more prominent. Decades of research on polymeric materials, stem cells, and three-dimensional printing contributed to the rapid progress of tissue engineering and regenerative medicine that envisages the potential future of organ transplantations. This perspective discusses the role of synthetic polymers in tissue engineering, their design and properties in relation to each type of application. Additionally, selected recent achievements of tissue engineering using synthetic polymers are outlined to provide insight into how they will contribute to the advancement of the field in the near future. In this way, we aim to provide a guide that will help scientists with synthetic polymer design and selection for different tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Section: Design Aspects Polymer Selection and Latest Advancesmentioning

confidence: 99%

Biocompatible Synthetic Polymers for Tissue Engineering Purposes

et al. 2022

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“…Furthermore, since the osteochondral interface involves both cartilage and subchondral bone, researchers have focused on the use of biphasic scaffolds carrying osteogenic and chondrogenic peptides, respectively. For instance, osteogenic peptide/TGF-β1 ( Wang et al, 2020 ) and HA bind (hyaluronic acid-binding peptide)/E3 (mineralizing peptide) ( Camacho et al, 2021 ) have been successfully used for osteochondral tissue regeneration.…”

Section: 3d Printingmentioning

confidence: 99%

3D Printing for Bone-Cartilage Interface Regeneration

Jiao

et al. 2022

Front. Bioeng. Biotechnol.

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Due to the vasculature defects and/or the avascular nature of cartilage, as well as the complex gradients for bone-cartilage interface regeneration and the layered zonal architecture, self-repair of cartilage and subchondral bone is challenging. Currently, the primary osteochondral defect treatment strategies, including artificial joint replacement and autologous and allogeneic bone graft, are limited by their ability to simply repair, rather than induce regeneration of tissues. Meanwhile, over the past two decades, three-dimension (3D) printing technology has achieved admirable advancements in bone and cartilage reconstruction, providing a new strategy for restoring joint function. The advantages of 3D printing hybrid materials include rapid and accurate molding, as well as personalized therapy. However, certain challenges also exist. For instance, 3D printing technology for osteochondral reconstruction must simulate the histological structure of cartilage and subchondral bone, thus, it is necessary to determine the optimal bioink concentrations to maintain mechanical strength and cell viability, while also identifying biomaterials with dual bioactivities capable of simultaneously regenerating cartilage. The study showed that the regeneration of bone-cartilage interface is crucial for the repair of osteochondral defect. In this review, we focus on the significant progress and application of 3D printing technology for bone-cartilage interface regeneration, while also expounding the potential prospects for 3D printing technology and highlighting some of the most significant challenges currently facing this field.

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“…One of the most critical obstacles in the clinical application of 3D bioprinting is the selection of an appropriate cell source, which should be safe, minimally invasive, fast and easy to expand (Amler et al, 2021). In addition, 3D scaffold surface functionalization usually requires post-processing treatment, which may lead to inevitable side effects or change the original morphology and shape of the scaffold (Camacho et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

3D Bioprinted Scaffolds for Tissue Repair and Regeneration

et al. 2022

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Three-dimensional (3D) printing technology has emerged as a revolutionary manufacturing strategy that could realize rapid prototyping and customization. It has revolutionized the manufacturing process in the fields of electronics, energy, bioengineering and sensing. Based on digital model files, powdered metal, plastic and other materials were used to construct the required objects by printing layer by layer. In addition, 3D printing possesses remarkable advantages in realizing controllable compositions and complex structures, which could further produce 3D objects with anisotropic functions. In recent years, 3D bioprinting technology has been applied to manufacture functional tissue engineering scaffolds with its ability to assemble complicated construction under precise control, which has attracted great attention. Bioprinting creates 3D scaffolds by depositing and assembling biological and/or non-biological materials with an established tissue. Compared with traditional technology, it can create a structure tailored to the patient according to the medical images. This conception of 3D bioprinting draws on 3D printing technology, which could be utilized to produce personalized implants, thereby opening up a new way for bio-manufacturing methods. As a promising tool, 3D bioprinting can create complex and delicate biomimetic 3D structures, simulating extracellular matrix and preparing high precision multifunctional scaffolds with uniform cell distribution for tissue repair and regeneration. It can also be flexibly combined with other technologies such as electrospinning and thermally induced phase separation, suitable for tissue repair and regeneration. This article reviews the relevant research and progress of 3D bioprinting in tissue repair and regeneration in recent years. Firstly, we will introduce the physical, chemical and biological characteristics of biological scaffolds prepared by 3D bioprinting from several aspects. Secondly, the significant effects of 3D bioprinting on nerves, skin, blood vessels, bones and cartilage injury and regeneration are further expounded. Finally, some views on the clinical challenges and future opportunities of 3D bioprinting are put forward.

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Spatial organization of biochemical cues in 3D-printed scaffolds to guide osteochondral tissue engineering

Abstract: Peptide-functionalized 3D-printed scaffolds drive mesenchymal stem cells (MSCs) differentiation towards osteogenesis or chondrogenesis based on the presence and organization of both cartilage-promoting and bone-promoting peptides.

Cited by 21 publications

References 100 publications

Biocompatible Synthetic Polymers for Tissue Engineering Purposes

Biocompatible Synthetic Polymers for Tissue Engineering Purposes

3D Printing for Bone-Cartilage Interface Regeneration

3D Bioprinted Scaffolds for Tissue Repair and Regeneration

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