Spatial Control of Cell Adhesion and Patterning through Mussel-Inspired Surface Modification by Polydopamine

Abstract

The spatial control and patterning of mammalian cells were achieved by using the universal adhesive property of mussel-inspired polydopamine (PDA). The self-polymerization of dopamine, a small molecule inspired by the DOPA motif of mussel foot proteins, resulted in the formation of a PDA adlayer when aqueous dopamine solution was continuously injected into poly(dimethylsiloxane) microchannels. We found that various cells (fibrosarcoma HT1080, mouse preosteoblast MC3T3-E1, and mouse fibroblast NIH-3T3) predominantly adhered to PDA-modified regions, maintaining their normal morphologies. The cells aligned in the direction of striped PDA patterns, and this tendency was not limited by the type of cell line. Because PDA modification does not require complex chemical reactions and is applicable to any type of material, it enables cell patterning in a simple and versatile manner as opposed to conventional methods based on the immobilization of adhesive proteins. The PDA-based method of cell patterning should be useful in many biomaterial research areas such as the fabrication of tissue engineering scaffolds, cell-based devices for drug screening, and the fundamental study of cell-material interactions.

Non-leaching antimicrobial surfaces through polydopamine bio-inspired coating of quaternary ammonium salts or an ultrashort antimicrobial lipopeptide

“…21 Confocal Raman microscopy demonstrated the appearance of two signals at 1360 and 1588 cm À1 , which are attributed to catechol C]O stretching vibrations. 34 Since the PDA coating is suspected to be redox active, we also analyzed the PDA-coated ITO surface by cyclic voltammetry (Fig. 1E).…”

Deposition Mechanism and Properties of Thin Polydopamine Films for High Added Value Applications in Surface Science at the Nanoscale

“…Patterned polydopamine arrays produced by injecting dopamine solutions in poly(dimethylsiloxane) micro-channels allowed the adhesion of various kinds of cells as fibrosarcoma HT1080, mouse preosteoblasts MC3T3-E1, and mouse fibroblasts NIH-3T3 [121]. The blood compatibility of nylon, cellulose, and polyethersulfone decorated with polydopamine films was demonstrated by means of platelet adhesion [122].…”

Biomimetic polymer-based Ag nanocomposites as a antimicrobial platform

“…The characterization of these materials surfaces has highlighted their potential application in various fields, including biosensors [7,25] and tissue engineering [26][27][28]. Recently, PDA-functionalized hybrid nanomaterials have been developed, which showed great potential in catalysis [29], medical metallic implants [30], and anti-fouling applications [16].…”