Spatial and temporal variation in denitrification and in the denitrifier community in a boreal lake

Rissanen, Antti J.; Tiirola, Marja; Ojala, Anne

doi:10.3354/ame01506

Cited by 34 publications

(34 citation statements)

References 86 publications

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“…Our study also showed the linkage between NO 3 − concentration and N 2 O accumulation, which is in agreement with previous work in boreal lakes [18]. Based on previously published N 2 production rates for five of the lakes in this study [29, 32] (unpublished results), the N 2 O production rates reported here correspond to 0.2−1.7% of the total gaseous N production (N 2 O/(N 2 +N 2 O) ratio). These values fall within the range of previously reported estimates (0.1–4.1%) for freshwater systems [33].…”

Section: Discussionsupporting

confidence: 93%

Genetic and Environmental Controls on Nitrous Oxide Accumulation in Lakes

et al. 2015

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We studied potential links between environmental factors, nitrous oxide (N2O) accumulation, and genetic indicators of nitrite and N2O reducing bacteria in 12 boreal lakes. Denitrifying bacteria were investigated by quantifying genes encoding nitrite and N2O reductases (nirS/nirK and nosZ, respectively, including the two phylogenetically distinct clades nosZ I and nosZ II) in lake sediments. Summertime N2O accumulation and hypolimnetic nitrate concentrations were positively correlated both at the inter-lake scale and within a depth transect of an individual lake (Lake Vanajavesi). The variability in the individual nirS, nirK, nosZ I, and nosZ II gene abundances was high (up to tenfold) among the lakes, which allowed us to study the expected links between the ecosystem’s nir-vs-nos gene inventories and N2O accumulation. Inter-lake variation in N2O accumulation was indeed connected to the relative abundance of nitrite versus N2O reductase genes, i.e. the (nirS+nirK)/nosZ I gene ratio. In addition, the ratios of (nirS+nirK)/nosZ I at the inter-lake scale and (nirS+nirK)/nosZ I+II within Lake Vanajavesi correlated positively with nitrate availability. The results suggest that ambient nitrate concentration can be an important modulator of the N2O accumulation in lake ecosystems, either directly by increasing the overall rate of denitrification or indirectly by controlling the balance of nitrite versus N2O reductase carrying organisms.

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Section: Discussionsupporting

confidence: 93%

Genetic and Environmental Controls on Nitrous Oxide Accumulation in Lakes

et al. 2015

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“…For DNA‐analysis and sediment characteristics, three replicate samples of the uppermost sediment (0–2 cm) were collected from each sampling point using Sandman sediment sampler, and stored at −20°C. Nitrate + nitrite (NO x − ) and ammonium (NH 4 + ) from water above the sediment surface (collected with Limnos sampler) were determined as in Rissanen and colleagues (). Wastewater discharge samples (100 ml of effluent water) were collected from all WWTPs.…”

Section: Methodsmentioning

confidence: 99%

Resistant ammonia‐oxidizing archaea endure, but adapting ammonia‐oxidizing bacteria thrive in boreal lake sediments receiving nutrient‐rich effluents

Aalto

Saarenheimo

Mikkonen

et al. 2018

Environmental Microbiology

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SummaryClimate change along with anthropogenic activities changes biogeochemical conditions in lake ecosystems, modifying the sediment microbial communities. Wastewater effluents introduce nutrients and organic material but also novel microbes to lake ecosystems, simulating forthcoming increases in catchment loadings. In this work, we first used 16s rRNA gene sequencing to study how the overall sediment microbial community responds to wastewater in six boreal lakes. To examine forthcoming changes in the lake biogeochemistry, we focused on the ammonia‐oxidizing archaea (AOA) and bacteria (AOB), and examined their functional and compositional community response to wastewater. Although we found the least diverse and least resistant prokaryotic communities from the most wastewater‐influenced sediments, the community changed fast toward the natural composition with the diminishing influence of wastewater. Each lake hosted a unique resistant AOA community, while AOB communities were adapting, responding to environmental conditions as well as receiving new members from WWTPs. In general, AOB dominated in numbers in wastewater‐influenced sediments, while the ratio between AOA and AOB increased when moving toward pristine conditions. Our results suggest that although future climate‐change‐driven increases in nutrient loading and microbial migration might significantly disrupt lake sediment microbiomes, they can promote nitrification through adapting and abundant AOB communities.

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“…Obviously, the spatial variation in the BCC in the sediment of small deep lakes (Rissanen et al 2011) or large deep lakes (e.g. Baikal, Constance, Kinneret; Pester et al 2004, Shubenkova et al 2005, Schwarz et al 2007, Deutzmann et al 2011) depends on the depth of the lake and, in the case of large lakes, the shape of the lake basin, leading to diverse conditions in the basins of these lakes.…”

Section: Discussionmentioning

confidence: 99%

Homogeneous microbial diversity in the upper sediment layers of a shallow lake

Tšertova¹,

Kisand

Baty³

et al. 2013

Aquat. Microb. Ecol.

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The variability and patchiness in composition of bacterial communities were studied in a large eutrophic boreal lake (280 km 2 ) with a mean depth of < 3 m. The present study was undertaken to determine the factors that influence species dispersal in the sediment environment of large shallow lakes and to test the hypothesis that species dispersal in the sediment is heterogeneous. Surficial sediment samples were collected according to both transect and 'log-wise' sampling designs. For the vertical slicing of sediment samples, steps were chosen according to average deposition rates. Bacterial community composition (BCC) was examined by denaturing gradient gel electrophoresis (DGGE) of PCR amplification products from DNA extracted from the total community. The relationships of the DGGE fingerprint to space, bacterial abundance, production, and dry weight of the sediment were analyzed using multivariate statistical methods. The BCC was found to be uniform over both the 1 to 1000 m ('log-wise') and kilometer (>10 km transect) scales. An increase in bacterial diversity from the river bed to mid-lake corresponded to the sediment type and was related to an increase in productivity. Vertically, the bacterial diversity decreased with sediment depth while the bacterial community in the upper 2 to 5 cm of sediment was almost homogeneous. These data suggest that BCC of organic-rich sediment is very homogeneous horizontally, and the upper centimeters of the sediment undergo frequent mixing, leading to similar homogeneity.

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Spatial and temporal variation in denitrification and in the denitrifier community in a boreal lake

Cited by 34 publications

References 86 publications

Genetic and Environmental Controls on Nitrous Oxide Accumulation in Lakes

Genetic and Environmental Controls on Nitrous Oxide Accumulation in Lakes

Resistant ammonia‐oxidizing archaea endure, but adapting ammonia‐oxidizing bacteria thrive in boreal lake sediments receiving nutrient‐rich effluents

Homogeneous microbial diversity in the upper sediment layers of a shallow lake

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