Spatial and temporal dynamics of nitrogen fixing, nitrifying and denitrifying microbes in an unfertilized grassland soil

Regan, Kathleen M.; Stempfhuber, Barbara; Schloter, Michael; Rasche, Frank; Prati, Daniel; Philippot, Laurent; Boeddinghaus, Runa S.; Kandeler, Ellen; Marhan, Sven

doi:10.1016/j.soilbio.2016.11.011

Cited by 85 publications

(58 citation statements)

References 90 publications

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“…()); as well as bacterial and archaeal abundances (qPCR on 16S rRNA; Regan et al . ()). These variables were used in statistical analyses to determine their explanatory power with respect to AMF OTU richness and community composition (see detailed list of environmental properties in Supporting Information Table S4).…”

Section: Methodsmentioning

confidence: 94%

“…Through an interdisciplinary approach, it was previously clarified that plant growth changes plot‐scale spatial heterogeneity of soil microorganisms during the vegetation period, and elucidated driving forces behind this observed microbial heterogeneity (Regan et al ., ). We linked existing measures of seasonal and spatial changes in plant diversity, abiotic soil properties and general microbial community composition (Regan et al ., , , ; Klaus et al ., ) to AMF diversity and community patterns. Using high‐throughput sequencing technology, this study aimed to answer the following questions: (i) how much variability in AMF alpha‐ and beta‐diversity exists on a spatial scale of 10 m × 10 m and a temporal scale of one season?…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have been conducted at broad geographical scales (Öpik et al, 2006;Hazard et al, 2013;Davison et al, 2015;Bouffaud et al, 2016Bouffaud et al, , 2017, but to date little is known about richness and occurrence patterns of AMF at or within plot scales (≤ 50 m × 50 m) in grassland ecosystems (Lekberg et al, 2012;Horn et al, 2014). One advantage of such small-scale study designs is the focus on environmental conditions and variations in plant communities within a specific habitat, thereby excluding overriding effects of large-scale heterogeneity at the landscape level (Berner et al, 2011;Regan et al, 2017). Thus, fundamental influences on AMF communities can be studied at such plot or subplot scales, ranging from centimetre to metre.…”

Section: Introductionmentioning

confidence: 99%

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Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

Goldmann

Boeddinghaus

Klemmer

et al. 2019

Environmental Microbiology

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Summary Soils provide a heterogeneous environment varying in space and time; consequently, the biodiversity of soil microorganisms also differs spatially and temporally. For soil microbes tightly associated with plant roots, such as arbuscular mycorrhizal fungi (AMF), the diversity of plant partners and seasonal variability in trophic exchanges between the symbionts introduce additional heterogeneity. To clarify the impact of such heterogeneity, we investigated spatiotemporal variation in AMF diversity on a plot scale (10 × 10 m) in a grassland managed at low intensity in southwest Germany. AMF diversity was determined using 18S rDNA pyrosequencing analysis of 360 soil samples taken at six time points within a year. We observed high AMF alpha‐ and beta‐diversity across the plot and at all investigated time points. Relationships were detected between spatiotemporal variation in AMF OTU richness and plant species richness, root biomass, minimal changes in soil texture and pH. The plot was characterized by high AMF turnover rates with a positive spatiotemporal relationship for AMF beta‐diversity. However, environmental variables explained only ≈20% of the variation in AMF communities. This indicates that the observed spatiotemporal richness and community variability of AMF was largely independent of the abiotic environment, but related to plant properties and the cooccurring microbiome.

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Section: Methodsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

Goldmann

Boeddinghaus

Klemmer

et al. 2019

Environmental Microbiology

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“…The concentration of ammonium in soil has been identified as an important factor driving the relative distributions of AOA and AOB [52]. In the investigated alpine forest, the abundance of AOA varied almost inversely with the measured ammonium concentration at all sampling altitudes, while that of AOB varied positively with ammonium concentration ( Table 2), suggesting that ammonium may drive the separation between AOA and AOB [53].…”

Section: Discussionmentioning

confidence: 95%

Soil Nitrogen Responses to Soil Core Transplanting Along an Altitudinal Gradient in an Eastern Tibetan Forest

Zhang

Wang²,

et al. 2018

Forests

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Abstract:To understand the differential effects of altitudinal gradient on soil inorganic nitrogen concentration and associated ammonia-oxidizingbacteria (AOB) and archaea (AOA), intact soil cores from a primary coniferous forest were in situ incubated in an alpine forest at a 3582-m altitude (A1) and transplanted to subalpine forests at a 3298-m altitude (A2) and 3023-m altitude (A3) on the eastern Tibetan Plateau. Transplant cooled the soil temperature of A2 but warmed the A3 soil temperature. Both AOA and AOB were found at the three altitudes. Compared to A1, A2 had greater AOA and AOB abundance, but A3 showed lower AOA abundance in organic soil. The AOA abundance was negatively correlated with ammonium concentration at all three altitudes, but AOB showed the reverse trend. Our results suggested that the soil nitrogen process responded differentially to soil core transplanting at different altitudes.

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“…compared to the other study sites surveyed by Bardelli et al (2017). Indeed, complex interactions between local-scale factors, such as soil properties and vegetation composition, are known to largely affect soil microbial communities and their spatial distribution in mountain environments (Ascher et al 2012;Regan et al 2017;Siles et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Chemical and microbiological changes in Norway spruce deadwood during the early stage of decomposition as a function of exposure in an alpine setting

Bardelli

Gómez-Brandòn

Fornasier³

et al. 2018

Arctic, Antarctic, and Alpine Research

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Alpine ecosystems are vulnerable to ever-changing environmental conditions, leading to shifts in vegetation distribution and composition with implications on soil functionality and carbon (C) turnover. Although deadwood represents an important global C stock, scarce information is available on how slope exposure influences the wood-inhabiting microbiota throughout the decomposition process in an Alpine setting. We therefore evaluated the impact of slope exposure (north-vs. south-facing sites) on physicochemical and microbiological properties (microbial abundance based on real-time PCR: fungal 18S rRNA, dinitrogen reductase [nifH]; microbial biomass: double strand DNA; and microbial activity: hydrolytic enzyme activities of the main nutrient cycles) of Picea abies wood blocks and the underlying soil in a field experiment in the Italian Alps during a three-year period. Overall, a higher abundance of fungi and nitrogen-fixing bacteria was recorded in the soil at the north-facing site where cooler and moister conditions were observed. In contrast, no exposure effects were found for these two microbial groups in the wood blocks, while their abundance increased over time, accompanied by more acidic conditions with progressing decay. The impact of exposure was also enzyme specific and time dependent for both the P. abies wood blocks and the underlying soil.ARTICLE HISTORY

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Spatial and temporal dynamics of nitrogen fixing, nitrifying and denitrifying microbes in an unfertilized grassland soil

Cited by 85 publications

References 90 publications

Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

Soil Nitrogen Responses to Soil Core Transplanting Along an Altitudinal Gradient in an Eastern Tibetan Forest

Chemical and microbiological changes in Norway spruce deadwood during the early stage of decomposition as a function of exposure in an alpine setting

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