SP1–DLEU1–miR-4429 feedback loop promotes cell proliferative and anti-apoptotic abilities in human glioblastoma

Chen, Ruwei; Liu, Lijun

doi:10.1042/bsr20190994

Cited by 18 publications

(11 citation statements)

References 49 publications

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“…it has also been suggested to play a pivotal role in the development of cervical cancer by sponging mir-125b (14). recently, mirnas have been demonstrated to be involved in the malignant behaviors of cancer by regulating cell growth and metastasis, including mir-4429 (15). lncrna linc00313 has been reported to modulate papillary thyroid cancer tumorigenesis by sponging mir-4429.…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA SNHG12 regulates cell proliferation, invasion and migration in endometrial cancer by targeting miR‑4429

Cai

Zhang

et al. 2020

Mol Med Rep

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long non-coding rna small nucleolar rna host gene 12 (SnHG12) has been demonstrated to be oncogenic. The aim of the present study was to examine the effects of SnHG12 on the progression of endometrial cancer (ec). The expression levels of SnHG12 and microrna (mir)-4429 were assessed in ec cell lines by reverse transcription-quantitative Pcr. Plasmids, including SnHG12 short hairpin rnas (shrnas), shrna negative control (nc), SnHG12 overexpression (oV), oV-nc, mir-4429 mimic and mimic-nc, were transfected into rl95-2 cells. Post-transfection, cell counting Kit-8, Transwell Matrigel and wound-healing assays were performed to assess cell proliferation, invasion and migration, respectively. cell cycle phase distribution was assessed by flow cytometry. The protein expression levels of matrix metalloproteinase (MMP)2 and MMP9 were detected by western blotting. mir-4429 target genes were predicted by bioinformatics analysis using target prediction online tools; the findings of this analysis were verified using a dual-luciferase reporter system. Identified as a target of miR-4429, SNHG12 was overexpressed in ec cell lines with decreased expression of mir-4429. Further experiments demonstrated that SnHG12 silencing and overexpression of mir-4429 markedly suppressed proliferation, migration and invasion of rl95-2 cells, arrested cells in the G 1 phase, and markedly downregulated the expression of MMP2 and MMP9. The opposite effects were observed in mir-4429 mimic-transfected rl95-2 cells after SNHG12 was overexpressed. The findings of the present study established the role of SnHG12 and mir-4429 in ec. Therefore, targeting the SnHG12/mir-4429 axis could serve as a potential future therapeutic target for treatment of ec.

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Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA SNHG12 regulates cell proliferation, invasion and migration in endometrial cancer by targeting miR‑4429

Cai

Zhang

et al. 2020

Mol Med Rep

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“…Furthermore, miR-4429 has been reported as a tumor suppressor in clear cell renal cell carcinoma (ccRCC), and its downregulation was associated with poor prognosis of ccRCC patients while its upregulation inhibited growth of ccRCC cells [ 16 ]. Likewise, silencing of miR-4429 was also found to increase the viability of glioblastoma multiforme cells [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway

Wang

Xie

et al. 2021

BMC Urol

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Background Emerging evidence suggests that microRNAs (miRNAs) play multiple roles in human cancers through regulating mRNAs and distinct pathways. This paper focused on the functions of miR-4429 in prostate cancer (PCa) progression and the molecules involved. Methods Expression of miR-4429 in PCa tissues and cells was determined. Upregulation of miR-4429 was introduced in PCa cells to examine its role in the malignant behaviors of cells. The putative target mRNA of miR-4429 involved in PCa progression was predicted from a bioinformatic system and validated through luciferase assays. Overexpression of distal-less homeobox 1 (DLX1) was further induced in cells to validate its implication in miR-4429-mediated events. The activity of Wnt/β-catenin pathway was determined. Results miR-4429 was poorly expressed in PCa tissues and cells. Artificial upregulation of miR-4429 significantly reduced proliferation, growth, invasion, migration and resistance to death of cancer cells and inactivated the Wnt/β-catenin pathway. DLX1 mRNA was found as a target of miR-4429. Upregulation of DLX1 restored the malignant behaviors of PCa cells which were initially suppressed by miR-4429, and it activated the Wnt/β-catenin pathway. Conclusion Our study highlights that miR-4429 inhibits the growth of PCa cells by down-regulating DLX1 and inactivating the Wnt/β-catenin pathway. This finding may offer novel insights into PCa treatment.

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“…Importantly, lncRNAs can regulate their transcriptional regulators in turn and thus form a positive feedback loop. For instance, SP1-DLEU1-miR-4429 positive feedback loop facilitates cell growth in glioblastoma (29,30). LncRNA HEGBC promotes gallbladder cancer progression by forming a positive feedback loop with IL11/STAT3 signaling pathway (31).…”

Section: Discussionmentioning

confidence: 99%

LMCD1 antisense RNA 1 (LMCD1-AS1) potentiates thyroid cancer cell growth and stemness via a positive feedback loop of LMCD1-AS1/miR-1287-5p/GLI2

Shao

et al. 2020

Ann Transl Med

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Background: LMCD1 antisense RNA 1 (LMCD1-AS1) is a certified oncogene in several tumour types.However, its role in thyroid cancer (THCA) remains unknown.Methods: The expression level of LMCD1-AS1 in THCA cells and the normal control cell was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The effects of LMCD1-AS1 knockdown on cell proliferation, migration and apoptosis were detected by colony formation assay, EdU assay, wound healing assay and TUNEL assay. Sphere formation assay was applied to assess sphere formation ability of THCA cells. Bioinformatics analysis and mechanism experiments, including ChIP assay, RIP assay and luciferase reporter assay were conducted to evaluate the downstream and upstream molecular mechanisms of LMCD-AS1.Results: A marked up-regulation of LMCD1-AS1 in THCA cells relative to normal control cells was found. LMCD1-AS1 silencing suppressed proliferation and migration but induced apoptosis in THCA cells. Moreover, LMCD1-AS1 knockdown reduced the sphere formation capacity of THCA cells. The transcriptional factor GLI family zinc finger 2 (GLI2) binds to LMCD1-AS1, which contributed to LMCD1-AS1 up-regulation in THCA cells. Cytoplasmic LMCD1-AS1 sponged a shared microRNA between LMCD1-AS1 and GLI2. GLI2 was inhibited bymiR-1287-5p and disinhibited by LMCD1-AS1.Conclusions: LMCD1-AS1exerts pro-tumorigenic function through sponging miR-1287-5p to elevate GLI2 expression in THCA development, constituting a feedback loop of LMCD1-AS1/miR-1287-5p/GLI2.

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SP1–DLEU1–miR-4429 feedback loop promotes cell proliferative and anti-apoptotic abilities in human glioblastoma

Cited by 18 publications

References 49 publications

Long non‑coding RNA SNHG12 regulates cell proliferation, invasion and migration in endometrial cancer by targeting miR‑4429

Long non‑coding RNA SNHG12 regulates cell proliferation, invasion and migration in endometrial cancer by targeting miR‑4429

MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway

LMCD1 antisense RNA 1 (LMCD1-AS1) potentiates thyroid cancer cell growth and stemness via a positive feedback loop of LMCD1-AS1/miR-1287-5p/GLI2

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