Soybean lecithin-based extender as an alternative for goat sperm cryopreservation

Vidal, Andréa Helena; Batista, André Mariano; Silva, Ellen Cordeiro Bento da; Gomes, W. A.; Pelinca, M. A.; Silva, Sildivane Valcácia; Guerra, Maria Madalena Pessoa

doi:10.1016/j.smallrumres.2012.07.022

Cited by 51 publications

(40 citation statements)

References 25 publications

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“…Sperm motility is one of the routine sperm quality parameters and is important as an indicator of attaining to the oocyte. In the previous studies, the motility rates of goat sperm cryopreserved with lecithin‐based extenders ranged between 11.0% and 58.4% (Adeyanju, Daramola, Olanite, & Awokola, 2018; Narwade, Mohanty, Bhakat, & Rahim, 2017; Salmani, Towhidi, Zhandi, Bahreini, & Sharafi, 2014; Vidal et al, 2013). Post‐thaw motility rates of our study in RTS groups agree with previous researches.…”

Section: Discussionmentioning

confidence: 99%

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post‐thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin‐based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 × 106 spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA‐FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post‐thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin‐based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.

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Section: Discussionmentioning

confidence: 99%

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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“…On the other hand, Vidal et al. () reported that no difference in acrosome integrity between SL‐based extenders and milk‐based extender. This discrepancy may be due to different species.…”

Section: Discussionmentioning

confidence: 99%

“…; Vidal et al. ). In most of the previous studies, commercial lecithin‐based extenders with unknown formulation were used and a few studies (Sharafi et al.…”

Section: Introductionmentioning

confidence: 98%

Optimization of Ram Semen Cryopreservation Using a Chemically Defined Soybean Lecithin‐Based Extender

Emamverdi

Zhandi

Shahneh

et al. 2013

Reprod Domestic Animals

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The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin-based semen extender as a substitute for egg yolk-based extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different extenders: (i) Tris-based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT extenders. The results showed that the proportion of live post-thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT extenders. In addition, SL1, SL1.5 and SL2.5 extenders resulted in significantly lower percentage of early-apoptotic sperm than that in EYT extender. There were no significant differences in different semen extenders for percentage of post-thawed necrotic and late-apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between extenders. In conclusion, SL1.5 extender was better than other extenders in most in vitro evaluated sperm parameters.

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“…Salmani et al [83] have suggested the use of soyabeanlecithin which is a suitable plant based cryoprotectant for caprine sperms. The beneficial effects of soyabean-lecithin as a substitute for egg yolk during cryopreservation of goat sperm are established in the works of Jiménez-Rabadán et al, Roof et al, Salmani et al, and Vidal et al [84][85][86][87].…”

Section: Cattlementioning

confidence: 99%

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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Lipid is an important constituent of cell membrane. Membrane lipid composition of spermatozoa has been correlated to different function. Many researchers have related membrane lipid with survival success after cryopreservation or cold shock. Sperm maturation and acrosome reactions are natural phenomenon, but cryopreservation or cold shock is not. Therefore, sperm cells are not programmed for such change and undergo stress. So the change in membrane lipid composition due to cold shock or cryopreservation may be looked upon as response of spermatozoa to a certain stressed condition. A significant body of research worked on the relationship between membrane lipid and fatty acid composition and ability of cell to tolerate adverse change in temperature. However, as the approach of different research groups was different, it is very difficult to compare the changes. Studies have been done with different species, ejaculated/seminal or epididymal sperm. Lipid analyses have been done with whole cell membrane isolated by different methods. Fatty acids estimated were from whole cell, plasma membrane, head membrane, or phospholipids. The cryopreservation condition, media composition, and diluents/cryoprotectants were also different. At this onset a comprehensive review is needed to cover changes of sperm membrane lipid composition of different species under different cryopreservation conditions.

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Soybean lecithin-based extender as an alternative for goat sperm cryopreservation

Cited by 51 publications

References 25 publications

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

Optimization of Ram Semen Cryopreservation Using a Chemically Defined Soybean Lecithin‐Based Extender

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

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