Sox31 is involved in central nervous system anteroposterior regionalization through regulating the organizer activity in zebrafish

Hu, Shengnan; Wu, Zhili; Yan, Yonggang; Li, Yiping

doi:10.1093/abbs/gmr025

Cited by 13 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The similar expression pattern of Sm‐Sox2 and Sm‐Sox19 observed in ventricular regions of the forebrain of the turbot (present results), suggests that both genes could have a similar role in early embryogenesis and in the development of the CNS, as it has been suggested in zebrafish (Adolf et al, ; Diotel et al, ; Germanà et al, ; Girard et al, ; Hu et al, ; Lam et al,; Lindsey et al, ; Okuda et al, ; Vriz et al, ) and other vertebrates (reviewed in Pevny & Nicolis, ; Wegner & Stolt, ).…”

Section: Discussionsupporting

confidence: 84%

“…Regarding Sox19 , which is specific of fishes (Okuda et al, ; Vriz & Lovell‐Badge, ), this gene has been identified in rice field eel (Liu & Zhou, ), fugu (Koopman, Schepers, Brenner, & Venkatesh, ), sturgeon (Hett & Ludwig, ), zebrafish (Okuda et al, ), sea bass (Navarro‐Martín, Galay‐Burgos, Piferrer, & Sweeney, ), turbot (Viñas et al, ), and the Nile tilapia (Wei et al, ). The Sox19 expression pattern observed in the brain of teleosts during embryogenesis and in adults suggests that it is involved in CNS development (Diotel et al, ; Girard, Crémazy, Berta, & Renucci, ; Hu, Wu, Yan, & Li, ; Navarro‐Martín et al, ; Okuda et al, ; Vriz, Joly, Boulekbache, & Condamine, ; Wei et al, ). Moreover, the Sox19 gene is also expressed in the gonads and muscle of some teleosts (Navarro‐Martín et al, ; Wei et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…The Sox19 expression pattern observed in the brain of teleosts during embryogenesis and in adults suggests that it is involved in CNS development (Diotel et al, 2015;Girard, Cr emazy, Berta, & Renucci, 2001;Hu, Wu, Yan, & Li, 2011;Navarro-Martín et al, 2012;Okuda et al, 2006;Vriz, Joly, Boulekbache, & Condamine, 1996;Wei et al, 2016).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Comparative expression patterns of Sox2 and Sox19 genes in the forebrain of developing and adult turbot (Scophthalmus maximus)

Taboada

Viñas

Adrio

2017

J of Comparative Neurology

View full text Add to dashboard Cite

The turbot, Scophthalmus maximus, belongs to the flatfishes (order Pleuronectiformes), which display substantial asymmetry of the olfactory organs and forebrain. Sox genes code for SRY-related HMG domain-bearing transcription factors involved in various developmental processes. Group B1 Sox genes as Sox2 and Sox19 appear to play major roles in neural development. Here, we characterized by in situ hybridization the developmental expression of Sox2 and Sox19 genes in metamorphic and postmetamorphic specimens and young adults of both sexes. Expression of S. maximus Sox2 (Sm-Sox2) and Sm-Sox19 mRNAs was detected in ependymal cells of different regions of the telencephalon, preoptic region, hypothalamus, and thalamus at all stages investigated. Sm-Sox2 expression but not Sm-Sox19 occurred in neurons located in particular regions such as the dorsal nucleus of the ventral telencephalon, the medial zone of the dorsal telencephalon, preoptic area and hypothalamus. Although Sm-Sox2 and Sm-Sox19 are expressed differentially in gonads, no sex differences in their expression were observed between male and female forebrains. We also investigated the topographical relation between Sox expression and cell proliferation using series double immunostained for a radial glial marker (BLBP) and cell proliferation marker (PCNA). Sm-Sox2 and Sm-Sox19 were strongly expressed in ependymal cells located in neurogenic niches revealed by the BLBP and PCNA immunostaining. Comparison with other teleosts indicates similar expression of Sox2 and Sox19 in the telencephalon, supporting conserved roles for both genes in teleost brains.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative expression patterns of Sox2 and Sox19 genes in the forebrain of developing and adult turbot (Scophthalmus maximus)

Taboada

Viñas

Adrio

2017

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…The SoxB1 transcription factors sox1/2/3/19a/31(19b) are found to take part in various processes during early embryogenesis [8,9]. We previously reported that translational blocking of sox31 leads to a dorsalized phenotype [10]. Here we show that splice blocking morpholinos (Sb MO) against sox31 generate a series of aberrant splicing variants, which likely encode dominant negative forms of sox31.…”

Section: Introductionmentioning

confidence: 75%

Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid‐Blastula Transition and induces apoptosis in zebrafish

Hu¹,

Yu²,

Zhang³

et al. 2011

FEBS Letters

View full text Add to dashboard Cite

a b s t r a c tHere we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6 hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage.

show abstract

“…37,38 We designed a light-activated splice-switching oligonucleotide targeting sox31 (also known as sox19b ), a member of the B1 Sox gene family that is responsible for many critical processes during zebrafish development, both before and after the midblastula transition (MBT). 39–41 During the blastulation stage, sox31 is expressed throughout the blastoderm, where it then assists in epiboly formation during the gastrulation stage. 42 Splice variants of sox31 have been previously generated with morpholino oligonucleotides (MO) targeting cryptic splice sites in sox31 .…”

Section: Resultsmentioning

confidence: 99%

Conditional Control of Alternative Splicing through Light-Triggered Splice-Switching Oligonucleotides

et al. 2015

View full text Add to dashboard Cite

The spliceosome machinery is composed of several proteins and multiple small RNA molecules that are involved in gene regulation through the removal of introns from pre-mRNAs in order to assemble exon-based mRNA containing protein-coding sequences. Splice-switching oligo-nucleotides (SSOs) are genetic control elements that can be used to specifically control the expression of genes through correction of aberrant splicing pathways. A current limitation with SSO methodologies is the inability to achieve conditional control of their function paired with high spatial and temporal resolution. We addressed this limitation through site-specific installation of light-removable nucleobase-caging groups as well as photocleavable backbone linkers into synthetic SSOs. This enables optochemical OFF → ON and ON → OFF switching of their activity and thus precise control of alternative splicing. The use of light as a regulatory element allows for tight spatial and temporal control of splice switching in mammalian cells and animals.

show abstract

Sox31 is involved in central nervous system anteroposterior regionalization through regulating the organizer activity in zebrafish

Cited by 13 publications

References 33 publications

Comparative expression patterns of Sox2 and Sox19 genes in the forebrain of developing and adult turbot (Scophthalmus maximus)

Comparative expression patterns of Sox2 and Sox19 genes in the forebrain of developing and adult turbot (Scophthalmus maximus)

Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid‐Blastula Transition and induces apoptosis in zebrafish

Conditional Control of Alternative Splicing through Light-Triggered Splice-Switching Oligonucleotides

Contact Info

Product

Resources

About