2013
DOI: 10.1039/c3ib20290a
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Abstract: Traditional cell-screening techniques such as FACS and MACS are better suited for large numbers of cells isolated from bulk tissue and cannot easily screen stem or progenitor cells from minute populations found in their physiological niches. Furthermore, these techniques rely upon irreversible antibody binding, potentially altering cell properties, including gene expression and regenerative capacity. To address these challenges, we have developed a novel, label-free stem-cell analysis and sorting platform capa… Show more

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Cited by 24 publications
(22 citation statements)
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“…Recent studies have emphasized molecular and functional heterogeneity within the satellite cell pool [6, 11, 26]. For example, satellite cells expressing higher levels of Pax7 have been shown to display lower metabolic activity, proliferate less, and possess an increased propensity to self-renew [28].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Recent studies have emphasized molecular and functional heterogeneity within the satellite cell pool [6, 11, 26]. For example, satellite cells expressing higher levels of Pax7 have been shown to display lower metabolic activity, proliferate less, and possess an increased propensity to self-renew [28].…”
Section: Introductionmentioning
confidence: 99%
“…Yet, within the non-hematopoietic, non-fibroadipogenic subset of muscle mononuclear cells, many surface marker schemes have been reported to positively enrich satellite cells. Some of the cell surface antigens employed are used independently of other positive markers, including VCam1, α7-integrin, NCam1, cMet, m-Cadherin, and Synd3/4 [5, 15, 18, 21, 24, 34], and some are used in combination, including β1-integrin and CXCR4 or α7-integrin and CD34 [11, 14, 19, 29, 32, 33, 35]. However, it remains unknown if all of these surface proteins are expressed on the same satellite cells.…”
Section: Introductionmentioning
confidence: 99%
“…Finally, antibodies (SSEA-1, SSEA-4, mouse IgG 3 , mouse IgM, Tra-1-81-all obtained from Biolegend, San Diego, CA), diluted to 1.33 µM for IgG-isotype antibodies (which are bound by Protein G) and to 556 nM for IgM-isotype antibodies (undiluted stock concentration, which are bound by Protein L), are linked to the surface by overnight incubation at 4 °C. This entire functionalization procedure provides antibody immobilization stability over an extended period of time, as previously demonstrated Chapman et al 2013;Jabart et al 2014) 2.3 COMSOL modeling of shear rates in microchannel…”
Section: Device Functionalizationmentioning
confidence: 71%
“…The glass surface enclosed by the microfluidic channel is silanized in a humid chamber using N-(3-triethoxysilylpropyl)-4-hydroxybutyramide (Gelest, Morrisville, PA), diluted in a stock solution of 0.01 % acetic acid in 95 % ethanol and 5 % DI water for 4 h at room temperature (RT), as done previously (Carbonaro et al 2008;Chapman et al 2013). Channels are then rinsed with stock solution and DI water and subsequently dried and cured for 2 h at 120 °C.…”
Section: Device Functionalizationmentioning
confidence: 99%
“…Likewise, Sherwood et al demonstrated similar FACS isolation of myogenic cells using positive markers CXCR4 and β1-integrin and negative markers CD45, Sca-1, and Mac-1, and cell purity was confirmed by growing the sorted cells into myotubes in cell culture [54]. Because of the heterogeneity of the satellite cell population, with certain sub-populations displaying unique surface marker profiles, various additional marker combinations specific to satellite cells have been identified [55,56]. Consistent with the work described above, Bosnakovski et al identified CD34 as a positive marker and CD45 as a negative marker for satellite cells sorted from a Pax7- ZsGreen + mouse [57].…”
Section: Purificationmentioning
confidence: 99%