Sorotipos de Streptococcus suis identificados em suínos com meningite no estado do Paraná

Rocha, Diana; Santos, Luis Fernando dos; Santos, Delcivan Lima dos; Costa, W.M.T.; Santos, Jósé Lúcio dos

doi:10.1590/s0102-09352012000200032

Cited by 6 publications

(10 citation statements)

References 9 publications

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“…Isolates presented a slight ten- Articulation, CNS, lung, 1 (11), 2 (37), 4 (1), pericardium, thoracic cavity 7(1), NT (1) SP 73 (42.0) Articulation, CNS, lung, 1 (2), 2 (56), 3 (10), pericardium, thoracic 7 (3), NT (2) cavity, peritoneum * PE = Pernambuco, BA = Bahia, RS = Rio Grande do Sul, GO = Goiás, PR = Paraná, MG = Minas Gerais, SC = Santa Catarina, SP = São Paulo. The high frequency of S. suis serotype 2 isolation, especially from the central nervous system, corroborates previous studies of swine infection in Brazil and worldwide (Pagnani et al 2002, Vela et al 2003, Del`Arco et al 2008, Rocha et al 2012. Also the high frequency of mrp + /epf + / sly + and mrp + /epfv/sly + genotypes corroborates previous reports of S. suis infection in Europe and China (Silva et al 2006, Wei et al 2009).…”

Section: Resultssupporting

confidence: 89%

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

et al. 2016

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Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates from distinct Brazilian states, from 1999 to 2004, and its molecular and serological characterization. A total of 174 isolates were studied. S. suis identification was confirmed by PCR and isolates were further serotyped and genotyped by SE-AFLP and amplification of virulence markers. Serotype 1, 2, 3, 4, 7, 18, 22 and 32 were identified among the studied isolates, and only 4% were characterized as non-typeable. The mrp + /epf + /sly + genotype was the most frequent. The SE-AFLP analysis resulted in 29 patterns distributed in three main clusters with over 65% of genetic similarity. Isolates presented a slight tendency to cluster according to serotype and origin; however, no further correlation with virulence genotypes was observed.

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Section: Resultssupporting

confidence: 89%

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

et al. 2016

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“…In Brazil, serotype 2 is described as the most prevalent, followed by serotypes 1 2 , 14, 7, and 9 [16][17][18][19]. The results of molecular serotyping in the present study corroborate the literature with a predominance of serotype 2/ 1 2 , which was identified in 86.0% (185/215) of strains from all sites and years of isolation.…”

Section: Discussionsupporting

confidence: 89%

“…The results of molecular serotyping in the present study corroborate the literature with a predominance of serotype 2/ 1 2 , which was identified in 86.0% (185/215) of strains from all sites and years of isolation. In addition, eight more serotypes were detected (3,6,7,8,18,27,28, and 1/14). To our knowledge, this is the first report of S. suis serotype 27 in Brazil.…”

Section: Discussionmentioning

confidence: 99%

Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016

et al. 2019

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Streptococcus suis remains an important challenge for the worldwide swine industry. Considering that Brazil is a major pork producer and exporter, proper monitoring of the pathogen and resistance rates are required. We present here the characterization of Brazilian S. suis strains isolated over a 15 year period by pulsed-field gel electrophoresis (PFGE) typing, capsular, virulence, and antimicrobial resistance profiling. Serotype prevalence revealed a predominance of serotype 2/ 1 2 followed by 3, 7, 1/14, 6, 8, 18, 28, and 27; the latter had not yet been reported in Brazil. Resistance profiling enabled the differentiation of nine profiles presenting resistance to three and up to eight antimicrobial classes. Even though an association between the most resistant strains and isolation year starting from 2009 was observed, a high frequency of multidrug-resistant strains isolated from 2001 to 2003 was also detected. This suggests that despite the isolation period, S. suis strains already presented high resistance selection pressure. A slight association of serotype 2/ 1 2 with some virulence profiles and PFGE pulsotypes was also identified. Nevertheless, no clonal dispersion or persistency of clones over the analyzed years and herds was detected.Pathogens 2020, 9, 31 2 of 15 shown that serotypes 20, 22, 26, 32, 33, and 34 do not belong to this species and should be classified as other bacterial species [6,7]. Moreover, novel nine capsular polysaccharide synthesis (cps) loci (NCLs) of non-typable S. suis strains have been identified based on DNA sequencing. Therefore, strict S. suis species currently comprise 38 serotypes [8].Among the virulence factors that have already been characterized in S. suis, most studied so far are the capsule, muramidase-released protein (MRP), the extracellular factor (EF), hemolysins including suilysin (SLY), plasminogen receptors, and arginine deiminase (arcA) [9].Considering antimicrobial susceptibility of S. suis strains, recent studies have described increases in resistance rates to some antimicrobial classes. Resistance to lincosamides and macrolides has been increasing, both for pigs and human strains, and resistance to sulfonamides and tetracycline showed high prevalence [8]. Resistance to cephalosporin was already described in Europe and China, but resistance prevalence to penicillin, ampicillin, and ceftiofur remains low in most countries [8,10].Emergence of multidrug resistant S. suis strains has also been described in humans and pigs, including asymptomatic animals, with highlight for the Asian epidemic clones [8]. Phenotypic and genetic studies suggest that swine may be reservoirs for the spread of antibiotic-resistant S. suis strains, which demands attention for the public health risk [8].Brazil is a major producer and exporter of pork, occupying for several years the fourth place as producer and exporter in the world [4]. This position demands attention to swine health issues. Currently, special efforts are required to reduce antimicrobial usage and monitor resistance rates ...

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“…Very few data are available regarding strains isolated in South America. A few reports on serotype 2 strains recovered from diseased pigs in Brazil have been published ( Costa et al , 2005 ; Doto et al , 2016 ; Rocha et al , 2012 ), though no human case has yet been reported. Human cases have been reported in Argentina ( Lopreto et al , 2005 ; Callejo et al ., 2014 ; Nagel et al , 2008 ) and, only once, in Chile ( Alarcon et al , 2013 ), but strains were not further characterized.…”

Section: Discussionmentioning

confidence: 99%

Streptococcus suis serotype 2 strains isolated in Argentina (South America) are different from those recovered in North America and present a higher risk for humans

Callejo

Zheng

et al. 2016

JMM Case Reports

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Introduction:Streptococcus suis serotype 2 is an important swine pathogen and emerging zoonotic agent causing meningitis and septicemia/septic shock. Strains are usually virulent (Eurasia) or of intermediate/low virulence (North America). Very few data regarding human and swine isolates from South America are available.Case presentation:Seventeen new human S. suis cases in Argentina (16 serotype 2 strains and a serotype 5 strain) are reported. Alongside, 14 isolates from pigs are analyzed: 12 from systemic disease, one from lungs and one from tonsils of a healthy animal. All human serotype 2 strains and most swine isolates are sequence type (ST) 1, as determined by multilocus sequence typing and present a mrp+/epf+/sly+ genotype typical of virulent Eurasian ST1 strains. The remaining two strains (recovered from swine lungs and tonsils) are ST28 and possess a mrp+/epf−/sly− genotype typical of low virulence North American strains. Representative human ST1 strains as well as one swine ST28 strain were analyzed by whole-genome sequencing and compared with genomes from GenBank. ST1 strains clustered together with three strains from Vietnam and this cluster is close to another one composed of 11 strains from the United Kingdom.Conclusion: Close contact with pigs/pork products, a good surveillance system, and the presence of potentially virulent Eurasian-like serotype 2 strains in Argentina may be an important factor contributing to the higher number of human cases observed. In fact, Argentina is now fifth among Western countries regarding the number of reported human cases after the Netherlands, France, the UK and Poland.

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Sorotipos de Streptococcus suis identificados em suínos com meningite no estado do Paraná

Cited by 6 publications

References 9 publications

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016

Streptococcus suis serotype 2 strains isolated in Argentina (South America) are different from those recovered in North America and present a higher risk for humans

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