Sonoporation as an Approach for siRNA delivery into T cells

Karki, A.; Giddings, Emily; Carreras, Ana; Champagne, Devin P.; Fortner, Karen A.; Rincón, Mercedes; Wu, Junru

doi:10.1016/j.ultrasmedbio.2019.06.406

Cited by 17 publications

(23 citation statements)

References 32 publications

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“…The first attempt includes the transport of antigen mRNA to dendritic cells, which afterward, via the expression of antigenic peptide, can act as DC vaccines against tumor cells in vivo ( 254 ). Research also concerned the transport of siRNA molecules to the primary T cells which resulted in a decreased methylation-controlled J protein expression ( 255 ). The Golgi-associated transmembrane protein downregulation is involved in increased resistance to specific drugs by inducing expression of the ABCB1 drug transporter via the c-Jun-related pathway.…”

Section: Physical Methods and Their Mechanismsmentioning

confidence: 99%

Chemotherapy and Physical Therapeutics Modulate Antigens on Cancer Cells

et al. 2022

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Cancer cells possess specific properties, such as multidrug resistance or unlimited proliferation potential, due to the presence of specific proteins on their cell membranes. The release of proliferation-related proteins from the membrane can evoke a loss of adaptive ability in cancer cells and thus enhance the effects of anticancer therapy. The upregulation of cancer-specific membrane antigens results in a better outcome of immunotherapy. Moreover, cytotoxic T-cells may also become more effective when stimulated ex-vivo toward the anticancer response. Therefore, the modulation of membrane proteins may serve as an interesting attempt in anticancer therapy. The presence of membrane antigens relies on various physical factors such as temperature, exposure to radiation, or drugs. Therefore, changing the tumor microenvironment conditions may lead to cancer cells becoming sensitized to subsequent therapy. This paper focuses on the therapeutic approaches modulating membrane antigens and enzymes in anticancer therapy. It aims to analyze the possible methods for modulating the antigens, such as pharmacological treatment, electric field treatment, photodynamic reaction, treatment with magnetic field or X-ray radiation. Besides, an overview of the effects of chemotherapy and immunotherapy on the immunophenotype of cancer cells is presented. Finally, the authors review the clinical trials that involved the modulation of cell immunophenotype in anticancer therapy.

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Section: Physical Methods and Their Mechanismsmentioning

confidence: 99%

Chemotherapy and Physical Therapeutics Modulate Antigens on Cancer Cells

et al. 2022

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“…T-cells are known to be difficult to transfect cells due to their limited phagocytic activity. Sonoporation has been shown to allow successful delivery of fluorescent siRNA into T-cells as a proof-of-concept study [371]. One paper has reported that peripheral blood withdrawn Tregs, the regulatory and suppressor T-cell subtype, could be transfected ex vivo with microbubble-loaded Foxp3-miRNA and re-infused to effectively suppress tumor growth in mice [324,372].…”

Section: Immunological Barriermentioning

confidence: 99%

Opening doors with ultrasound and microbubbles: Beating biological barriers to promote drug delivery

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Engelen

et al. 2021

Advanced Drug Delivery Reviews

125

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“…Ultrasound-mediated membrane poration, also called sonoporation, was shown to enable membrane permeabilization by deploying acoustic pressure waves in combination with cavitating microbubbles (Kooiman et al, 2020;Lentacker et al, 2014). Karki et al recently proved that sonoporation could be used to deliver siRNA to both murine and human T cells with ~60% efficiency, while reporting cell viabilities of ~65% for murine T cells post-sonoporation (trypan blue cell counting) (Karki et al, 2019). Inertial cavitation and collapse of these large microbubbles in proximity of cells, however, can easily affect T cell membrane integrity and cause low cell survival (Harizaj et al, 2020;Kooiman et al, 2020).…”

Section: Soluporationmentioning

confidence: 99%