Some Gammaproteobacteria are enriched within CD14+ macrophages from intestinal lamina propria of Crohn’s disease patients versus mucus

Abstract

Crohn's disease causes chronic inflammation in the gastrointestinal tract and its pathogenesis remains unclear. In the intestine of Crohn's disease patients, CD14 + CD11 + CD163 low macrophages contribute to inflammation through the induction of Th17 cells and production of inflammatory cytokines; the CD14 + CD11c + 163 high fraction is anti-inflammatory through the production of IL-10 in normal cases. In this report, the 16S rRNA gene amplicon sequencing method was used to identify bacteria that are specifically present in intestinal CD14 + CD11c + macrophages of crohn's disease patients. Bacteria present in intestinal CD14 + CD11c + macrophages and mucus of crohn's disease patients were separated into different clusters in principal coordinates analysis. There was a statistically significant increase in the relative composition of CD14 + CD11c + macrophages from mucus in two phyla (Proteobacteria [p = 0.01] and Actinobacteria [p = 0.02]) and two families (Moraxellaceae [p < 0.001] and Pseudomonadaceae [p = 0.01]). In addition, OTU-1: Acinetobacter and OTU-8: Pseudomonadaceae tended to concentrate in the CD14 + CD11c + CD163 low subset, whereas OTU-10: Proteus, OTU-15: Collinsella tended to concentrate more in the CD14 + CD11c + CD163 high subset than the other subset and mucus.Crohn's disease causes chronic inflammation in the gastrointestinal tract and is known as inflammatory bowel disease (IBD) together with ulcerative colitis, yet fundamental therapy has not yet been established. Inflammatory reactions are usually controlled in the intestinal tract and intestinal homeostasis is maintained. In IBD, chronic inflammation is thought to occur by maintaining an excessively activated state of immune cells through the involvement of several factors, including genetic factors, environmental factors, immune abnormality, and intestinal bacteria 1,2 .In the intestinal tract of Crohn's disease, CD14 + macrophages are increased compared to normal intestine. CD14 + macrophages are more ability to produce inflammatory cytokines such as IL-23, TNF-α, and IL-6 than CD14 − macrophages 3 . CD14 + macrophages also have the ability to induce Th17 cells, but in the intestines of

Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort

“…The reduction in Agathobaculum, which exhibits effects on gut motility regulating ability, could be a contributory factor to the increased severity and frequency of GI dysmotility symptoms in this particular cohort (Kenna et al, 2021), as well as other PwP cohorts (Kaye et al, 2006;Knudsen et al, 2017;Li et al, 2019;Cirstea et al, 2020). On the other hand, Gammaproteobacteria, a pro-inflammatory bacterium that produces endotoxins and is associated with GI disorders such as Crohn's disease (Sekido et al, 2020), and Synergistetes (Synergistia) a bacterium involved in GI infections (Vartoukian et al, 2007), were significantly increased in our PwP cohort. Together, these findings support a significantly altered, pro-inflammatory gut microbiome in PwP.…”

Acinetobacter calcoaceticus is Well Adapted to Withstand Intestinal Stressors and Modulate the Gut Epithelium

“…Elevated levels of Acinetobacter in the setting of IBD has been observed in several studies ( Gophna et al, 2006 ; Lucke et al, 2006 ; Leung et al, 2014 ; Kevans et al, 2015 ; Tang et al, 2015 ; Sjoberg et al, 2017 ; Sekido et al, 2020 ; He et al, 2021 ; Qi et al, 2022 ). Two studies found high abundance of Acinetobacter in pediatric patients with newly diagnosed ulcerative colitis ( Kevans et al, 2015 ; Sjoberg et al, 2017 ); providing potential evidence that Acinetobacter species could be contributing to the onset of intestinal inflammation in genetically susceptible patients.…”

“…This study found that Acinetobacter levels significantly correlated with microbial pathways in actively inflamed colitis tissue, suggesting a potential causal relationship between Acinetobacter and intestinal inflammation. Another study identified Acinetobacter in the CD14 + CD11c + CD163low subset macrophages in the lamina propia of ulcerative colitis patients ( Sekido et al, 2020 ), which indicates these microbes were able to bypass the epithelial barrier. In our intestinal organoid model, we found that several A. calcoaceticus strains stimulated pro-inflammatory cytokine expression and suppressed mucin production.…”

“…Apart from these studies, little is known regarding the factors that influence Acinetobacter colonization and little data exists on the effects of A. calcoaceticus . The importance of Acinetobacter in the gut is highlighted by the fact that Acinetobacter species are elevated in certain disease states including ulcerative colitis, a subset of inflammatory bowel disease (IBD) ( Gophna et al, 2006 ; Lucke et al, 2006 ; Leung et al, 2014 ; Kevans et al, 2015 ; Tang et al, 2015 ; Sjoberg et al, 2017 ; Sekido et al, 2020 ; He et al, 2021 ; Qi et al, 2022 ). In this study, we sought to examine the ability of A. calcoaceticus strains to survive environmental stressors found in the gut, interact with other gut microbes and utilize dietary components.…”

A Doença De Crohn Em Decorrência Da Desregulação Do Sistema Imunológico

“…Assim, o paciente pode chegar a desenvolver hemorragias, estenoses, perfurações intestinais e até mesmo um carcinoma de colo retal. (SEKIDO et al, 2020;SILVA et al, 2020) Dessa forma, pode-se dizer que esta revisão integrativa de literatura tem como objetivo analisar a influência do sistema imunológico na origem, desenvolvimento e agravamento da Doença de Crohn, enfatizando o papel das células de defesa e seus efeitos na fisiopatologia da comorbidade.…”