Somatotopic Organization and Temporal Characteristics of Cerebrocortical Excitation in Response to Nasal Mucosa Stimulation With and Without an Odor in the Rat: An Optical Imaging Study

Zama, Manabu; Hara, Yaeko; Fujita, Satoshi; Kaneko, Tadayoshi; Kobayashi, Masayuki

doi:10.1016/j.neuroscience.2018.02.042

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Although stimulation of the nasal tip and nasal mucosa was performed with air puff stimulation, each initial response showed a small spatial variation. The averaged initial response to the nasal tip was located caudally to that to the nasal mucosa, in agreement with our previous study (Zama et al., 2018).…”

Section: Resultssupporting

confidence: 92%

“…The diffusion of puffed air should be taken into consideration. For example, air puff applied to the nasal mucosa must be diffused to the upper pharynx (Zama et al., 2018). Although some initial responses were observed far from other initial responses in air puff stimulation, the area of initial responses almost overlapped in the comparison between electrical stimulation and air puff stimulation.…”

Section: Discussionmentioning

confidence: 99%

“…The temperature (23 ± 2°C) and humidity (55 ± 5%) in the animal room were controlled with a 12 h light/dark cycle (lights on at 0700 h; off at 1900 h). Optical imaging with VSD (RH‐1691, Optical Imaging, New York, NY, USA) was performed as previously described (Fujita et al., 2019; Nakamura et al., 2015; Noma et al., 2020; Zama et al., 2018; Zama et al., 2019). Briefly, the rats received an atropine methyl bromide injection (0.5 mg/kg, i.p.)…”

Section: Methodsmentioning

confidence: 99%

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Asymmetrical organization of oral structures in the primary and secondary somatosensory cortices in rats: An optical imaging study

Kirihara

Zama

Fujita

et al. 2022

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In rodents, the representation of the body surface in the primary somatosensory cortex (S1) forms a mirror image along the ventral border of the S1 in the secondary somatosensory cortex (S2). Sensory information from the oral region is processed in the S1 and the border region between the S2 and insular oral region (IOR). We examined the relationship between somatosensory representations in the S1 and S2/IOR using optical imaging with a voltage‐sensitive dye in urethane‐anesthetized rats. In reference to the rhinal fissure and middle cerebral artery, we made a somatosensory map by applying electrical or air puff stimulation. The initial neural excitation in the S1 to facial structures, including the eyebrow, cornea, pinna, whisker pad, nasal tip, and nasal mucosa, spread toward the ventral area, putatively the S2. The initial cortical responses in the S1 to oral structures, including the lower lip, tongue, and teeth, were spatially separated from those in the S2/IOR. The representation of the tongue center, tongue tip, mandibular molar pulp, mandibular incisor pulp, and mandibular incisor periodontal ligament were almost linearly arranged from caudal to rostral in both S1 and S2/IOR. The lower lip was represented in the dorsal area from the representation of teeth and tongue in both S1 and S2/IOR. The representations of maxillary teeth were caudal and dorsal to the representations of mandibular teeth in the S1 and S2/IOR, respectively. These results suggest that the representation of oral structures in the S1 formed a non‐mirror image, not a mirror image, in the S2/IOR.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Asymmetrical organization of oral structures in the primary and secondary somatosensory cortices in rats: An optical imaging study

Kirihara

Zama

Fujita

et al. 2022

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“…Optical imaging was performed as previously described (3–6, 13, 19–21). Briefly the sham and pl-ION model rats were anesthetized with urethane (1.5 g/kg, i.p.…”

Section: Methodsmentioning

confidence: 99%

Preceding Administration of Minocycline Suppresses Plastic Changes in Cortical Excitatory Propagation in the Model Rat With Partial Infraorbital Nerve Ligation

et al. 2019

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Neuropathic pain is known to be attributable to the injured nerve, a postoperative problem induced by surgery. The infraorbital nerve (ION), a branch of the trigeminal nerve, innervates to the facial and oral regions and conveys somatosensory information to the central nervous system. The partial ligation of ION (pl-ION) is a method to mimic chronic trigeminal neuropathic pain and behavioral abnormality. To counteract induction of such abnormal pain, the effective pharmacological treatment is desired. Although recent studies have revealed the molecular mechanisms regarding chronic pain, estimation of the effectiveness of the pharmacological treatment has not been well-provided especially in the central nervous system so far. Here we examined whether pl-ION induces plastic changes in the cerebral cortex and investigated effects of minocycline on the cortical plastic changes. We performed the pl-ION to Wistar male rats (4–5 weeks old), and confirmed a mechanical nocifensive behavior in response to the mechanical stimulation with von-Frey filaments. The withdrawal threshold to mechanical stimuli of the whisker pad was decreased 1 day (1 d) after pl-ION, which continued up to 14 d after pl-ION, suggesting that pl-ION model rats presented allodynia and enhanced the response sustained at least for 14 d after pl-ION. Next, cerebrocortical activities were evaluated 3 d after pl-ION (3d-pl-ION) by the optical imaging with a voltages-sensitive dye, RH1691, to quantify the response to electrical stimulation of the whisker pad skin, mandibular molar dental pulp, and mentum skin. Electrical stimulation to the whisker pad skin induced smaller excitation in the primary sensory cortex (S1) of 3d-pl-ION in comparison to that in the sham. In contrast, cerebral cortical responses to the mandibular molar dental pulp and mentum skin stimuli increased both in S1, and the secondary somatosensory and insular oral region (S2/IOR) after pl-ION. Administration of minocycline (30 mg/kg/d) from 1 d before to 2 d after pl-ION partially recovered the pl-ION-induced changes in cortical excitation in S1 and S2/IOR in 3d-pl-ION. These results suggest that somatosensory and insular cortical excitation is changed by pl-ION, and the preceding injection of minocycline counteracts the plastic changes in the cortical activities.

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Application of oxytocin with low-level laser irradiation suppresses the facilitation of cortical excitability by partial ligation of the infraorbital nerve in rats: An optical imaging study

et al. 2020

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Somatotopic Organization and Temporal Characteristics of Cerebrocortical Excitation in Response to Nasal Mucosa Stimulation With and Without an Odor in the Rat: An Optical Imaging Study

Cited by 5 publications

References 31 publications

Asymmetrical organization of oral structures in the primary and secondary somatosensory cortices in rats: An optical imaging study

Asymmetrical organization of oral structures in the primary and secondary somatosensory cortices in rats: An optical imaging study

Preceding Administration of Minocycline Suppresses Plastic Changes in Cortical Excitatory Propagation in the Model Rat With Partial Infraorbital Nerve Ligation

Application of oxytocin with low-level laser irradiation suppresses the facilitation of cortical excitability by partial ligation of the infraorbital nerve in rats: An optical imaging study

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