Somatic embryogenesis — the gate to biotechnology in conifers

Zoglauer, Kurt; Behrendt, Ulrike; Rahmat, Adi; Roß, H.; Taryono, Taryono

doi:10.1007/978-3-7091-6040-4_11

Cited by 12 publications

(8 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In conifers, in vitro embryogenic induction rate was associated with several factors including culture environment, choice of explants, genotype and plant growth regulators (Zoglauer, 2003;Stasolla et al, 2003). The embryogenic induction in A. angustifolia was observed after 30 days culture.…”

Section: Resultsmentioning

confidence: 99%

“…The mechanism of embryogenic cultures induction is not fully understood. According to Zoglauer (2003), during the induction phase, cells cease their program of specialization and start a new developmental program. The specialization program is negatively affected by the methylation of DNA for which the auxins may play a fundamental role (Lo Schiavo et al, 1989).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures

Steiner

Vieira

Maldonado³

et al. 2005

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures

Steiner

Vieira

Maldonado³

et al. 2005

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Plant regeneration by SE has been obtained in several Pinus species (Zoglauer et al 2003), but to our knowledge it has not been reported for Stone pine (Pinus pinea L.). Stone pine is one of the most important tree species in the Mediterranean ecosystem, and is used for different purposes, such as environmental restoration (afforestation of coastal areas and continental dunes) and ornamental planting.…”

Section: Introductionmentioning

confidence: 95%

Plant regeneration in Stone pine (Pinus pinea L.) by somatic embryogenesis

Carneros¹,

Celestino²,

Klimaszewska

et al. 2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Regeneration of plants by somatic embryogenesis (SE) was achieved in Stone pine (Pinus pinea), one of the most characteristic tree species of the Mediterranean ecosystem. The initial explants were megagametophytes containing zygotic embryos from five selected half-sib families collected at different dates over 2 consecutive years. Rates of extrusion and initiation of SE differed in both years. However, qualitative patterns were very similar: for most families, the responsive developmental window was from late cleavage polyembryony to early cotyledonary stage. The highest overall mean frequencies of extrusion and SE initiation (7 and 0.9%, respectively, for the five families and the eight 2006 collections) were obtained on a modified Litvay's medium with 9 lM 2,4-D and 4.5 lM BAP, supplemented with L-glutamine and casein hydrolysate. Families showed large differences in frequencies of SE initiation from year to year. Only seven embryogenic lines were induced in 2005, representing three of the five families tested, whereas 34 lines from all the families were obtained in 2006. Proliferation of embryonal masses (EM) was significantly improved when they were subcultured after dispersing in liquid medium and collected on filter paper disks, instead of being subcultured as small clumps. This effect showed a significant interaction with genotype. Several preconditioning treatments and culture media combinations were tested for embryo development and maturation. The high proliferation rate of EM hampered somatic embryo development. However, up to 42 mature embryos from different lines of three of the five families were obtained, 23 of them germinated and seven converted into somatic seedlings.

show abstract

“…Actualmente, se ha conseguido inducir con éxito la embriogénesis somática en un número cada vez mayor de especies y explantos de inicio, principalmente en coníferas como Pinus taeda, Abies alba y Larix decidua, lo que ha propiciado su aplicación a escala operacional para la propagación masiva de genotipos selectos (Zoglauer et al, 2003). En el caso de N. alpina se ha conseguido inducir exitosamente masas proembriogénicas y regeneración de embriones somáticos viables desde explantos cotiledonares extraídos desde semillas maduras provenientes del huerto semillero clonal Huillilemu, ubicado en la provincia de Valdivia, décima región de Chile (Castellanos et al, 2005).…”

Section: Introductionunclassified

Proliferación de embriones somáticos de Nothofagus alpina: efecto de la fuente de nitrógeno orgánico exógena

Castellanos

Sánchez‐Olate

Wehenkel

et al. 2016

MYB

View full text Add to dashboard Cite

rESumENSe probó el efecto de algunas fuentes de nitrógeno orgánico como caseína hidrolizada y los aminoá-cidos L-Glutamina y L-Serina en el proceso de embriogénesis somática secundaria directa, empleando como explantes embriones somáticos de Nothofagus alpina inducidos desde cotiledones aislados de semillas maduras. Los mayores porcentajes de embriogénesis secundaria directa fueron obtenidos en medio base BTM y vitaminas MS, sin reguladores del crecimiento vegetal y en este medio más ANA, BAP y caseína hidrolizada. Sin embargo, el peso fresco de las masas de proliferación fue superior en presencia de una fuente adicional de nitrógeno orgánico. Bajo el tratamiento con reguladores del crecimiento y caseína hidrolizada también se produjo la mayor cantidad promedio de embriones producidos por explanto, aunque no hubo diferencias significativas con el tratamiento que contuvo L-Glutamina, donde el crecimiento de callo fue significativamente menor. Mediante subcultivos alternados entre medio base y medio con ANA, BAP y caseína hidrolizada, se logró la proliferación del cultivo embriogénico de manera directa, estable durante más de dos años y con baja incidencia de callo. PALABRAS CLAVE:Embriogénesis somática, estado de desarrollo, micropropagación, reguladores del crecimiento. ABSTrACTThe effect of some sources of organic nitrogen such as hydrolyzed casein and the aminoacids L-glutamine and L-Serine were tested in the process of direct secondary somatic embryogenesis, in which somatic embryos of Nothofagus alpina -induced from isolated cotyledons of mature seeds-were used as explants. The highest percentages of direct secondary embryogenesis were obtained in BTM base medium plus MS vitamins, without plant growth regulators, and in this medium plus NAA, BAP and hydrolyzed casein. However, the fresh weight of the proliferated mass was greatest in the presence of an additional source of organic nitrogen. The highest average number of embryos per explant was also produced in the treatment with growth regulators and hydrolyzed casein, although the number was not significantly higher than in the L-Glutamine treatment, in which significantly less callus growth occurred. Alternate use of base medium and NAA, BAP and hydrolyzed casein medium during subcul-

show abstract

Somatic embryogenesis — the gate to biotechnology in conifers

Cited by 12 publications

References 87 publications

Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures

Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures

Plant regeneration in Stone pine (Pinus pinea L.) by somatic embryogenesis

Proliferación de embriones somáticos de Nothofagus alpina: efecto de la fuente de nitrógeno orgánico exógena

Contact Info

Product

Resources

About