Somatic embryogenesis in plantain banana

Grapin, Agnès; Schwendiman, Jacques; Teisson, Claude

doi:10.1007/bf02823133

Cited by 51 publications

(27 citation statements)

References 16 publications

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“…However, conversion of these embryos into plants did not occur, probably due to a lack of development of the shoot apical meristem. Similar results were obtained by Lee et al [23] and Grapin et al [24] with banana somatic embryos and Nickle and Yeung [25] with Daucus carota L (Fig. 2).…”

Section: Resultssupporting

confidence: 90%

Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants

Titov¹,

Bhowmik²,

Mandal³

et al. 2006

American J. of Biochemistry and Biotechnology

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Abstract:The study was undertaken to examine banana cv. Kanthali floral bud apex as an alternative source material for in vitro propagation because huge number of explants die due to microbial contamination in case of shoot tip explants. Contamination free cultures were established by treating the floral bud explants with 0.1% HgCl 2 for 6 min. This study found that inflorescence tissues of experimental plant was almost contamination free but was high in phenolic compounds. Phenolic compounds secretion was successfully stopped by pre-soaking them in an antioxidant solution of 0.125% potassium citrate: citrate. After antioxidant treatment the floral bud explants were cultured on MS medium supplemented with different concentrations and combinations of BA+Kn+IAA/IBA+15%CW. Compact, white/greenish white callus was formed in different amount at all concentrations after 3 weeks of culture. All were again subcultured at same medium and after another 30-35 days at 2.0 mg L 1 BA+2.0 mg L 1 Kn+2.0 mg L 1 IAA+15%CW some callus showed embryogenic structure.

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Section: Resultssupporting

confidence: 90%

Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants

Titov¹,

Bhowmik²,

Mandal³

et al. 2006

American J. of Biochemistry and Biotechnology

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“…The choice of auxin is very important in the induction process and can affect the frequency and morphology of somatic embryos (Lazzeri et al 1987, Levi & Sink 1991, Rodriguez & Wetzstein 1994, 1998. Somatic embryogenesis of banana cultivars of different groups has been successfully achieved (Novak et al 1989, Dedh'a et al 1991, Côte et al 1996, Escalant et al 1994, Grapin et al 1996, however, the conversion into plants is frequently low, thus limiting its association with genetic transformation techniques. The characterization of the different stages of the embryogenic process can help to detect possible limiting steps as well as to locate the embryogenic regions in the explant.…”

Section: Introductionmentioning

confidence: 99%

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Filippi

Appezzato‐da‐Glória

2001

Rev. bras. Bot.

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-(Histological changes in banana explants, cv. Nanicão (Musa spp. Group AAA), submitted to different auxins for induction of somatic embryogenesis). The present work analyzes the behavior of banana explants, cv. Nanicão (Musa spp. Group AAA) regarding somatic embryogenesis induction treatments with several auxins. Longitudinal segments of shoot meristematic apices of micropropagated banana plantlets cultivated and rooted in vitro were introduced in culture medium containing dicamba, picloram, 2,4-D or NAA in different concentrations. Explant samples were collected at 0, 7 and 10 days and prepared for light microscopy. Histological sections were used for comparison of the histological changes occurring after induction treatment with different auxins. Embryogenic response was observed only in treatments with picloram or dicamba, with distinct embryogenic regions observed at 14 and 21 days in culture, respectively. Histological sections of embryogenic regions of the explant at 26 days in culture revealed the formation of meristematic regions, structures with multiple root meristems, and somatic embryos at early globular stages. Embryo-like structures morphologically similar to Musa balbisiana zygotic embryos were sectioned and showed a lack of apical meristems and absence of procambial differentiation. These results indicate the induction of non-functional somatic embryos and the need for more studies on developmental aspects and maturation treatments for optimization of the process.RESUMO -(Alterações histológicas em explantes de bananeira, cv. Nanicão (Musa spp. Grupo AAA) submetidos a diferentes auxinas para indução de embriogênese somática). O presente trabalho analisa o comportamento de explantes de bananeira 'Nanicão' (Musa spp. Grupo AAA) a tratamentos de indução com diferentes auxinas, visando à obtenção de embriogênese somática. Segmentos longitudinais de ápices meristemáticos caulinares de plântulas de bananeira micropropagadas e enraizadas in vitro foram introduzidos em meio de cultura contendo dicamba, picloram, 2,4-D ou NAA. Amostras dos explantes aos 0, 7 e 10 dias foram coletadas e preparadas para microscopia óptica. Cortes histológicos foram realizados nesses explantes, comparando-se as alterações observadas após os tratamentos com diferentes auxinas. Respostas embriogênicas foram observadas somente nos tratamentos com picloram e dicamba, sendo possível a observação visual de regiões embriogênicas aos 14 e 21 dias de cultivo, respectivamente. Cortes histológicos de regiões embriogênicas dos explantes, aos 26 dias, revelaram a formação de regiões meristemáticas compostas aparentemente por múltiplos meristemas radiculares e embriões nas fases iniciais do estágio globular. Cortes histológicos de estruturas morfologicamente semelhantes a embriões zigóticos de Musa balbisiana revelaram ausência dos meristemas apicais e não diferenciação procambial. Os resultados indicam a indução de embriões somáticos não-funcionais e, consequentemente, a necessidade de maiores estudos visando otimizar o process...

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“…The initial features of somatic embryos obtained in C. amada were translucent globular in appearance, which were similar to somatic embryos of banana (Escalant et al 1994;Cote et al 1996;Grapin et al 1996Grapin et al , 2000Navarro et al 1997;Becker et al 2000;Ganapathi et al 2001;Khalil et al 2002). Translucent globular shape was an important visible stage during early somatic embryo development.…”

Section: Histological Analysismentioning

confidence: 87%

Direct and Indirect Somatic Embryogenesis in Mango Ginger (Curcuma amada Roxb.)

Shajahan

Raju

Thilip

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Somatic embryogenesis is a developmental restructuring of somatic cells in the embryogenic pathway and forms a basis for the concept of totipotency in plant cells. Understanding the process of transition from vegetative to embryogenic cells and factors involved in the somatic embryogenesis and subsequent plant development represents a challenge for plant tissue culture studies in any selected species. Although several reviews have been published dealing somatic embryogenesis in several plant species, there is no recent information on somatic embryogenesis in mango ginger (Curcuma amada). Two different types of somatic embryogenetic pathways are available in mango ginger. They are indirect somatic embryogenesis (IDSE) and direct somatic embryogenesis (DSE). Indirect somatic embryogenesis occurs in friable embryogenic callus from leaf sheath explants that undergo an extreme proliferation before the development of somatic embryos, whereas in the direct somatic embryogenesis, two-step system of culture was followed. Initially, leaf sheath explants were subjected to a pretreatment under dark condition before they were transferred to the subculture medium for embryo development. Histological and scanning microscopic studies showed that the formation of translucent globular embryogenic cell suspension is an important visible stage during early somatic embryogenesis through indirect method. In the direct pathway, upon pretreatment, epidermal and subepidermal cells of the leaf sheath explants formed globular and elongated structures. The presence of clear protoderm in the globular embryos and procambial structures are crucial visible stages.

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Somatic embryogenesis in plantain banana

Cited by 51 publications

References 16 publications

Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants

Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Direct and Indirect Somatic Embryogenesis in Mango Ginger (Curcuma amada Roxb.)

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