Somatic embryogenesis from immature zygotic embryos of oil palm

Abstract

Immature zygotic embryos at different developmental stages were used for callus induction and regeneration studies. Immature embryos excised from fruits 77, 91, 100, 114, 128, 140 and 193 days after pollination and mature embryos were cultured on modified Y3 medium containing 500 mg 1 -~ cysteine, 0.5% (w/v) PVP-40, 500 p~M 2,4-D and 0.3% (w/v) charcoal. Compact embryogenic tissue began differentiating directly from embryo explants after 2 weeks of culture. The percentage of embryos forming compact embryogenic tissue ranged from 28.6% for 91-day-old embryos to 0% for 140-day-old and older embryos. Friable embryogenic tissue was observed in callus cultures derived from 100-day-old embryos. Although both compact and friable embryogenic tissues were successfully isolated, normal embryo and plantlet development was observed only from friable embryogenic tissue.Abbreviations: ABA-abscisic acid, 2,4-D-2,4-dichlorophenoxyacetic acid, NAA-naphthaleneacetic acid, PVP -polyvinylpyrollidone

Embryogenic calli induced in interspecific (Elaeis guineensis x E. oleifera) hybrid zygotic embryos

“…100 embryos per progeny were cultivated in Petri dishes (10 per plate) for two months and in 200 mL flasks (5 calli per flask) after that. The procedure described by Teixeira et al (1993) to induce somatic embryogenesis in immature tenera (E. guineensis, type dura x psifera commercial hybrids) zygotic embryos was then applied, except that Murashige and Skoog (1962) salts and vitamins were used. The number of embryos developing calli and plumules was registered and the results submitted to the Z test (SigmaStat v. 02).…”

Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

“…leaves (Texeira et al 1993;Texeira et al 1994). The frequencies for complete plant regeneration from some explants are still inefficient (Rival et al 1999).…”

Controle da calogênese do pau-brasil in vitro

“…Nesse caso, o aumento da concentração de auxinas tem sido indicado como estratégia para contornar o problema. Aumentando a concentração de 2,4-D em 50 vezes, passando de 10 ìM (sem carvão ativado), para 500 ìM (com 0,3% de carvão ativado) Teixeira et al (1993) conseguiram resultados satisfatórios em palmeira-de-óleo (Elacis guineenses). Resultado semelhante foi verificado por Biasi et al (1994), trabalhando com a propagação do abacateiro in vitro; o ácido ascórbico e o ácido cítrico também não foram eficientes no controle da oxidação de C. echinata.…”