Somatic embryogenesis assay from maternal explants of three olive cultivars (Olea europaea L.)

Toufik, Ibrahim; Mazri, Mouaad Amine; Guenoun, Farhate; Belkoura, I.

doi:10.1016/j.nbt.2012.08.336

Cited by 1 publication

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“…Cytokinins have the tendency to regulate cell proliferation and division during plant development ( Kieber and Schaller 2018 ). In this perspective, the exogenous supplementation of cytokinins such as BAP and zeatin enhances the process of cell division during the in vitro growth process ( Toufik et al 2014 ). Furthermore, cytokinins act as regulators of cytokinin-response genes that are activated in inner or outer cortex cells during the nodule initiation process ( Kieber and Schaller 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Delineation of the impacts of varying 6-benzylaminopurine concentrations on physiological, biochemical and genetic traits of different olive cultivars under in vitro conditions

Zhao,

Khatoon,

Matloob Javed

et al. 2024

AoB PLANTS

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The plant growth regulator 6-benzylaminopurine (BAP) is an important component of plant nutrient medium with tendency to accelerate physiological, biochemical and molecular processes in woody plants such as olive. To date, limited knowledge is available on the role of BAP in mediating physiological, biochemical, and genetic activities in olives under in vitro conditions. To cover this research gap, the current study was conducted with the objective of studying the role of BAP in regulating physiological traits (chlorophyll, CO2 assimilation), antioxidant enzymes (superoxide dismutase, catalase, and peroxidase), metabolic contents (starch, sucrose, and flavonoids) and gene expression (OeRbcl, OePOD10, OeSOD10, OeCAT7, OeSS4, OeSuSY7, OeF3GT, and OeChlH) under varying concentrations (0, 0.5, 1.5 and 2.5 mg L-1) within the provided in vitro conditions. The explants obtained from different olive cultivars (‘Leccino’, ‘Gemlik’, ‘Moraiolo’, ‘Arbosana’) were cultured on olive medium (OM) provided with different BAP concentrations using a two-factorial design, and data were analyzed statistically. All traits increased significantly under in vitro conditions due to increasing concentrations of BAP; however, this increase was more dramatic at 2.5 mg L−1 and the least dramatic at 0.5 mg L−1. Moreover correlation, principal component analysis (PCA) and heatmap cluster analysis confirmed significant changes in the paired association and expression of traits with changing BAP concentration and type of olive cultivars. Likewise, the expression of all genes varied due to changes in BAP concentration in all cultivars, corresponding to variation in physiological and biochemical traits. Moreover, the spectrographs generated via scanning electron microscopy further indicated the variations in the distribution of elements in olive leaf samples due to varying BAP concentrations. Although all cultivars showed a significant response to in vitro varying concentrations of BAP, the response of Arbosana was statistically more significant. In conclusion, the current study proved the dynamic impact of the varying BAP concentrations on regulating the physiological, biochemical, and molecular attributes of olive cultivars.

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Section: Introductionmentioning

confidence: 99%