1994
DOI: 10.2135/cropsci1994.0011183x003400050043x
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Abstract: Identifying appropriate tissue culture and plant regeneration systems for agronomically important cultivars is often an important step in developing gene introduction techniques. Culture conditions for somatic embryogenesis and plant regeneration of ‘Tifton 9’ bahiagrass (Paspalum notatum Fluegge L.) from leaf‐stem cross sections are described. Cultures were initiated and maintained in the dark on Schenk and Hildebrandt medium containing 3% sucrose and 6.6 mg L−1 dicamba. Plant regeneration was initiated by tr… Show more

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Cited by 29 publications
(11 citation statements)
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“…This technology offers opportunities to improve bahiagrass. While tissue culture and regeneration systems have been developed in bahiagrass (Bovo and Mroginski 1986;Akashi et al 1993;Shatters et al 1994), transformation and the recovery of transgenic plants have not been reported. Improvement of forage nutritive value by genetic engineering has been demonstrated in several forages, including alfalfa (Ni et al 1994) and in maize (He and Smith, submitted).…”
Section: Introductionmentioning
confidence: 99%
“…This technology offers opportunities to improve bahiagrass. While tissue culture and regeneration systems have been developed in bahiagrass (Bovo and Mroginski 1986;Akashi et al 1993;Shatters et al 1994), transformation and the recovery of transgenic plants have not been reported. Improvement of forage nutritive value by genetic engineering has been demonstrated in several forages, including alfalfa (Ni et al 1994) and in maize (He and Smith, submitted).…”
Section: Introductionmentioning
confidence: 99%
“…However, application of either technique is dependent on the availability of a regenerative (i.e. embryogenic or organogenic) cell culture procedure (Gray, 1992;Shatters et al, 1994).…”
Section: Introductionmentioning
confidence: 99%
“…However, as stated by other investigators (Marousky and West 1990;Akashi et al 1993), there is a need for a solution to the problem posed by season-dependent availability of fresh living material to initiate callus induction, since flowering in P. simplex, as in most Paspalum species, is restricted to July-September in the northern hemisphere. Moreover, as highlighted by Shatters et al (1994), the regeneration capability of callus may be lost after several months of culture. The use of seedling-derived hypocotyls can bypass this drawback and provide the possibility of obtaining fresh regenerable callus at any time and in a relatively short time.…”
Section: Resultsmentioning
confidence: 99%